Zeiss LSM 980 with Airyscan 2 confocal microscope system

配备 Airyscan 2 共焦显微镜系统的 Zeiss LSM 980

• 批准号: 10177119
• 负责人: Iain McPherson Cheeseman
• 金额: $ 60万
• 依托单位: WHITEHEAD INSTITUTE FOR BIOMEDICAL RES
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-15 至 2022-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177119
• 关键词: 3-Dimensional; 9 year old; Address; Area; Biological; Biomedical Research; Cell Differentiation process; Cell division; Collection; Computer software; Data Set; Event; Fluorescence Recovery After Photobleaching; Funding; Germ Cells; Hour; Image; Imaging technology; Institutes; Interruption; Lasers; Maintenance; Malignant Neoplasms; Metabolic; Microscope; Microscopy; Mission; Nerve Degeneration; Optics; Organoids; Outcomes Research; Photobleaching; Phototoxicity; Positioning Attribute; Regulation; Research Personnel; Research Project Grants; Resolution; Sample Size; Sampling; Scanning; Services; Specimen; System; Technology; Thick; Time; Tissues; United States National Institutes of Health; cell type; contrast imaging; detector; fluorophore; instrument; instrumentation; live cell imaging; molecular dynamics; novel diagnostics; novel therapeutics; software systems; stem cells; success

Project Summary/Abstract The Whitehead Institute requests $600,000 for the purchase of a critically-needed Zeiss LSM 980 with Airyscan 2 laser scanning confocal microscope (“Zeiss 980AS2”), to be located in the W.M. Keck Facility for Biological Imaging (“Keck Facility”) at the Whitehead Institute for Biomedical Research (“Whitehead”). The instrument requested is of great importance, as it will replace a nine year old Zeiss LSM 710 NLO (non-linear optics; “Zeiss710”) that is currently the most heavily used microscope in the Keck Facility and provide transformative new imaging technologies that are currently unavailable at Whitehead or in the Keck Facility. For each of the past 3 years, the Zeiss710 averaged 1400 hours of use by 40 researchers from 25 different labs. Zeiss has informed us that guaranteed service for the Zeiss710 will end in 2022. Replacement of this critical instrumentation prior to that time is important to minimize interruptions caused by excessive maintenance issues. Other microscopy facilities in our area are unable to accommodate the substantial amount of use the Zeiss710 currently supports, and also lack the critical new capabilities required for the proposed research projects. The Zeiss 980AS2 will provide robust confocal capabilities for the diverse research projects described in this proposal, such as enabling optical sectioning to generate high-contrast images in thick specimens, generating 3D datasets, and conducting photomanipulation to analyze molecular dynamics, such as FRAP (fluorescence recovery after photobleaching). Critically, the requested Zeiss 980AS2 will provide new cutting-edge technologies essential for addressing many outstanding questions from the 10 NIH-funded researchers presented in this proposal. The Airyscan 2 detector and software will bring the first super-resolution capabilities to the Keck Facility. The 32-channel spectral detector will allow simultaneous imaging of up to ten fluorophores, enabling studies that investigate cell type differentiation in organoids and tissues. The high-efficiency GaAsP detectors will enable imaging of dim samples and reduce photobleaching and phototoxicity during live-cell imaging. The Airyscan 2 8Y Multiplex option and Z-piezo stage allow for extremely fast image collection, enabling larger sample sizes for large volume scanning. The Zeiss ZEN Blue System software that controls the Zeiss 980AS2 will substantially simplify currently arduous tasks, such as multi-position tile scanning and finding rare events. By expanding the experimental approaches and capabilities available to researchers, the Zeiss 980AS2 will greatly enhance and facilitate the success of many NIH-funded projects, including the analysis of neural degeneration, cancer, germ cell differentiation, metabolic regulation, cell division, and stem cell identification. Acquisition of the Zeiss LSM 980 with Airyscan 2 will allow researchers to address these important biomedical issues, and the outcomes from this research will include new diagnostics and therapeutics, in accordance with the mission of the NIH.

项目总结/摘要 怀特黑德研究所要求60万美元用于购买急需的蔡司LSM 980， Airyscan 2激光扫描共聚焦显微镜（“Zeiss 980 AS 2”），将位于W.M.凯克设施， 生物成像（“凯克设施”）在怀特黑德生物医学研究所（“怀特黑德”）。的 所要求的仪器非常重要，因为它将取代使用了9年的蔡司LSM 710 NLO（非线性 光学;“Zeiss 710”），这是目前凯克设施中使用最多的显微镜，并提供 目前在Whitehead或Keck工厂无法获得的变革性新成像技术。 在过去的3年中，Zeiss 710平均每年被来自25个不同国家的40名研究人员使用1400小时。 labs.蔡司已通知我们，蔡司710的保修服务将于2022年结束。更换本 在此之前的关键仪器是重要的，以尽量减少中断所造成的过度 维护问题。我们地区的其他显微镜设施无法容纳大量的 蔡司710目前支持的使用量，也缺乏所需的关键新功能， 提出的研究项目。蔡司980 AS 2将为不同的应用提供强大的共焦功能。 该提案中描述的研究项目，例如使光学切片能够产生高对比度 厚标本中的图像，生成3D数据集，并进行光操作以分析分子 动力学，如FRAP（光漂白后的荧光恢复）。 最重要的是，所要求的蔡司980 AS 2将提供解决以下问题所必需的新尖端技术： 在这份提案中，10位NIH资助的研究人员提出了许多悬而未决的问题。Airyscan 2 探测器和软件将为凯克设施带来第一个超分辨率能力。的32通道 光谱探测器将允许同时成像多达10个荧光团，使研究，调查 类器官和组织中的细胞类型分化。高效率GaAsP探测器将使成像 使样品变暗并减少活细胞成像过程中的光漂白和光毒性。Airyscan 2 8Y 多路选择和Z-压电载物台可实现极快的图像采集， 大容量扫描。Zeiss ZEN Blue System软件控制着Zeiss 980 AS 2， 简化当前繁重的任务，例如多位置瓦片扫描和查找罕见事件。通过扩大 蔡司980 AS 2将极大地提高和增强研究人员的实验方法和能力， 促进了许多NIH资助的项目的成功，包括神经变性，癌症，细菌， 细胞分化、代谢调节、细胞分裂和干细胞鉴定。收购蔡司LSM 980与Airyscan 2将使研究人员能够解决这些重要的生物医学问题， 根据NIH的使命，这项研究将包括新的诊断和治疗方法。