PROTEOMIC ANALYSIS OF THE HUMAN KINETOCHORE

人类动粒的蛋白质组学分析

• 批准号: 8171375
• 负责人: Iain McPherson Cheeseman
• 金额: $ 0.08万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171375
• 关键词: Affinity; Affinity Chromatography; Computer Retrieval of Information on Scientific Projects Database; Cultured Cells; Funding; Grant; Human; Institution; Kinetochores; Proteins; Proteomics; Research; Research Personnel; Resources; Source; United States National Institutes of Health; human tissue; novel; protein complex; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are currently investigating human kinetochore composition and organization using an affinity tagging strategy to isolate known and novel human kinetocore proteins and kinetochore protein complexes. We have developed a combined approach to examine the cellular localization of a protein and conduct a high affinity purification of that protein from human tissue culture cells (Localization and Affinity Purification - LAP tag). Using this approach, we have isolated more than 20 human kinetochore proteins, including at least 10 novel kinetochore proteins. This ongoing analysis will attempt to characterize the full range of human kinetochore proteins and examine their interactions and organization.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我们目前正在使用 亲和力标记策略，以隔离已知和新型人类动力学蛋白质和 动力学蛋白复合物。 我们已经开发了一种合并的方法来检查 蛋白质的细胞定位，并从中进行高亲和力纯化。 人体组织培养细胞（定位和亲和力纯化 - 膝上标签）。 使用此 方法，我们已经隔离了20多种人动物学蛋白，其中包括至少10个 新型动力学蛋白。 这种正在进行的分析将试图表征整个范围 人类动物学蛋白质并检查其相互作用和组织。