PROTEOMIC ANALYSIS OF THE HUMAN KINETOCHORE

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 我们目前正在研究人类动粒的组成和组织， 亲和标记策略以分离已知和新的人激动核心蛋白， 动粒蛋白复合物。 我们已经制定了一个综合的方法来检查 蛋白质的细胞定位，并进行该蛋白质的高亲和纯化， 人组织培养细胞（定位和亲和纯化-HPLTAG）。 使用此 方法，我们已经分离出超过20个人类动粒蛋白，包括至少10个 新的动粒蛋白。 这一正在进行的分析将试图描述全系列 人类动粒蛋白质，并检查它们的相互作用和组织。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are currently investigating human kinetochore composition and organization using an affinity tagging strategy to isolate known and novel human kinetocore proteins and kinetochore protein complexes. We have developed a combined approach to examine the cellular localization of a protein and conduct a high affinity purification of that protein from human tissue culture cells (Localization and Affinity Purification - LAP tag). Using this approach, we have isolated more than 20 human kinetochore proteins, including at least 10 novel kinetochore proteins. This ongoing analysis will attempt to characterize the full range of human kinetochore proteins and examine their interactions and organization.