PROTEOMIC ANALYSIS OF THE HUMAN KINETOCHORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们目前正在研究人类动粒的组成和组织，使用 亲和标记策略分离已知和新的人类动核蛋白和 动粒蛋白复合体。我们已经开发了一种综合方法来检查 蛋白质的细胞定位并进行高亲和力纯化 人组织培养细胞(定位和亲和纯化-LAP标签)。使用这个 方法，我们已经分离了20多个人类动粒蛋白，其中至少有10个 新的动粒蛋白。这项正在进行的分析将试图全面描述 并研究它们之间的相互作用和组织结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are currently investigating human kinetochore composition and organization using an affinity tagging strategy to isolate known and novel human kinetocore proteins and kinetochore protein complexes. We have developed a combined approach to examine the cellular localization of a protein and conduct a high affinity purification of that protein from human tissue culture cells (Localization and Affinity Purification - LAP tag). Using this approach, we have isolated more than 20 human kinetochore proteins, including at least 10 novel kinetochore proteins. This ongoing analysis will attempt to characterize the full range of human kinetochore proteins and examine their interactions and organization.