Flow cytometer Cell Sorter for Biosafety Level-3 Facility

生物安全三级设施流式细胞仪细胞分选仪

• 批准号: 10177283
• 负责人: ANKITA GARG
• 金额: $ 54.7万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177283
• 关键词: 2019-nCoV; Address; Antigen-Antibody Complex; Area; Biological Assay; Biomedical Research; Bordetella; Brucella melitensis; Burkholderia mallei; Burkholderia pseudomallei; Cells; Communicable Diseases; Containment; Diagnostic; Ebola; Funding; Goals; HIV; Immune response; Immunity; International; Investigation; Laboratories; Maintenance; Mycobacterium tuberculosis; Organism; Pathogenesis; Pathogenicity; Pathology; Preclinical Testing; Process; Research; Research Personnel; Sampling; Sorting - Cell Movement; Speed; Therapeutic; United States Department of Agriculture; United States National Institutes of Health; Universities; Viral; biosafety level 3 facility; co-infection; disease transmission; experimental study; hemorrhagic fever virus; influenzavirus; instrument; novel vaccines; pathogen; population health; programs; respiratory; respiratory pathogen; transmission process; vaccine development; vaccine efficacy; vaccine evaluation

PROJECT SUMMARY/ABSTRACT Investigators at UGA have established national and international recognition for their research on high- containment pathogens of global importance. UGA investigators are working towards understanding pathogenesis and developing newer vaccines, therapeutics and diagnostics to confer sustained immunity to re- challenge with homologous or heterologous pathogen strains. However, to advance these programs, multiparametric flow cytometer with high speed sorting is critical to formulate assays which assess vaccine efficacy, disease transmission, host response, and pre-clinical testing of vaccine/therapeutics/diagnostic candidate(s). This capability is currently lacking at the University of Georgia. The major users for the proposed flow cytometer sorter comprise NIH-funded researchers from three Departments at UGA; Infectious Diseases, Population Health and Pathology. Additional users are investigators of United States Department of Agriculture. Currently, multiple flow cytometers and sorters are available at UGA, and three are equipped with Biosafety Cabinets for sorting infectious cells. However, none of the existing cytometers or sorters can be used for un-fixed/live samples that are processed in high-containment / Biosafety Level-3 (BSL3) or BSL3-Ag laboratories. Acquisition of the proposed flow cytometer-sorter will provide new capabilities that will allow investigators to address complex immunological research problems in the area of infectious diseases. Specific areas of investigation that will directly benefit by obtaining the proposed instrument are: 1) immune response and vaccine development for Brucella melitensis, Mycobacterium tuberculosis, Bordetella spp, influenza virus, SARS-CoV-2 and several others, 2) immune response to Human Immunodeficiency Virus (HIV) – Mycobacterium tuberculosis co-infection, 3) understanding of the transmission of respiratory pathogens, 4) host range and pathogenesis of respiratory viral and bacterial co-infection, 5) pathogenesis and immune response to Burkholderia mallei and Burkholderia pseudomallei and hemorrhagic fever viruses Ebola, Lassa and SARS-CoV-2. The lack of a cytometer-sorter in a high-containment facility has hindered UGA researchers ability to perform critical experiments with highly pathogenic organisms. The instrument will therefore facilitate the realization of research goals and maintenance of NIH support for biomedical research.

项目摘要/摘要 UGA的研究人员对高密度脂蛋白的研究在国内和国际上都得到了认可 遏制具有全球重要性的病原体。UGA的调查人员正在努力了解 致病机制和开发新的疫苗、治疗和诊断方法以增强持续免疫力 挑战同源或异源病原菌菌株。然而，为了推进这些计划， 高速分选的多参数流式细胞仪是制定评估疫苗的分析方法的关键 疫苗/治疗/诊断的有效性、疾病传播、宿主反应和临床前测试 候选人(S)。佐治亚大学目前缺乏这种能力。建议的主要用户 流式细胞仪分选器由来自UGA三个部门的NIH资助的研究人员组成；传染病， 人口健康与病理学。其他用户是美国司法部的调查人员 农业。目前，UGA提供了多个流式细胞仪和分选器，其中三个配备了 用于分离感染细胞的生物安全柜。然而，现有的细胞仪或分选仪都不能使用 对于在高含物/生物安全级别3(BSL3)或BSL3-Ag中处理的未固定/活样本 实验室。收购拟议的流式细胞仪分选器将提供新的能力，将使 研究人员解决传染病领域复杂的免疫学研究问题。特定的 将直接受益于获得拟议仪器的研究领域包括：1)免疫反应 以及布鲁氏菌、结核分枝杆菌、波氏杆菌、流感病毒、 SARS-CoV-2和其他几种病毒，2)对人类免疫缺陷病毒(HIV)的免疫反应- 结核分枝杆菌混合感染，3)了解呼吸道病原体的传播，4) 呼吸道病毒和细菌混合感染的宿主范围和发病机制5)发病机制和免疫 对马来伯克霍尔德氏菌、假鼻疽伯克霍尔德氏菌和出血热病毒埃博拉、拉萨的反应 和SARS-CoV-2。在一个高度封闭的设施中缺乏细胞分选器，这阻碍了UGA的研究人员 对高致病性生物进行关键实验的能力。因此，该文书将促进 实现研究目标并维持NIH对生物医学研究的支持。