Acquisition of a Beckman-Coulter Optima Analytical Ultracentrifuge

Beckman-Coulter最优分析型超速离心机的研制 项目摘要/摘要： 该提案详细说明了为Beckman-Coulter Optima分析性超速离心机提供资金的申请，该超速离心机具有多个 波长紫外-可见光吸收和瑞利干涉检测光学器件，以及必要的附件。 该仪器将安装在约翰逊研究基金会结构生物学和生物物理核心 (JRFSBBC)由佩雷尔曼生物化学和生物物理系监督的设施 宾夕法尼亚大学医学院。该仪器将通过以下方式支持NIH资助的研究 宾夕法尼亚大学和大费城地区其他研究机构的调查人员，合作研究 与来自美国各地的NIH和NSF资助的研究人员合作，在那里类似的技术和专业知识 以及研究生和博士后培训生的培训。这一新仪器将取代 ~25岁的Beckman XL-目前服务于核心的分析性超速离心机(AUC)。新的 该仪器代表了分析超速离心法的重要进展，并使 正在出现利用多波长数据收集的方法；这在较旧的 乐器。这些技术进步包括改进的波长重复性、极大地改进了信令比 噪声和更快的扫描速度，这将在研究中提供更高的精度 溶液中大分子缔合和形状的热力学。这些研究项目将会是 先进的是范围广泛和所有高影响的，包括艾滋病毒药物发现，癌症，抗生素耐药性， 整体膜蛋白药物靶点，神经退行性疾病，染色质结构。这个 所寻求的技术相对较新，在全国范围内的学术环境中几乎没有这样的工具 (自2017年初以来)，预计将影响一大批内部和外部用户。这台仪器将 由JRFSBBC非常有经验的团队管理，JRFSBBC成立于2016年，在已经 庞大而稳健的研究环境。

Acquisition of a Beckman-Coulter Optima Analytical Ultracentrifuge Project Summary/Abstract: This proposal details a funding request for a Beckman-Coulter Optima analytical ultracentrifuge with multi- wavelength UV-Visible absorption and Rayleigh interference detection optics, with necessary accessories. The instrument will be installed in the Johnson Research Foundation Structural Biology and Biophysics Core (JRFSBBC) Facility that is overseen by the Department of Biochemistry and Biophysics at the Perelman School of Medicine of the University of Pennsylvania. The instrument will support NIH-funded research by investigators at Penn and other research institutions in the Greater Philadelphia region, collaborative research with NIH and NSF-funded researchers from across the US where similar technology and expertise is not available, and the training of graduate students and postdoctoral trainees. This new instrument will replace a ~25-year-old Beckman XL-A analytical ultracentrifuge (AUC) that currently serves the core. The new instrument represents an important advance in analytical ultracentrifugation and enables the application of arising methods that leverage multiwavelength data collection; this is currently not possible with the older instrument. These technical advances include improved wavelength reproducibility, vastly improved signal-to- noise, and faster scanning rates, which together will provide superior precision in the study of the thermodynamics of macromolecular associations and shape in solution. The research projects that will be advanced are broad in scope and all high-impact, including HIV drug discovery, cancer, antibiotic resistance, integral membrane protein drug targets, neurodegenerative disorders, and chromatin structure. The technology sought is relatively new, with few such instruments available in the academic setting nationwide (since early 2017) and is expected to impact a large group of intra- and extramural users. The instrument will be managed by the very experienced team at the JRFSBBC, which was established in 2016, within an already large and robust research environment.