Analytical ultracentrifuge with absorbance and interference optics.

具有吸光度和干涉光学器件的分析超速离心机。

• 批准号: 10177341
• 负责人: Christopher L Colbert
• 金额: $ 38.44万
• 依托单位: NORTH DAKOTA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2022-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177341
• 关键词: Address; Agonist; Area; Autophagocytosis; Biological Process; Biophysics; Cell surface; Characteristics; Collaborations; Core Facility; Doctor of Philosophy; Enzymes; Faculty; Fee-for-Service Plans; Fees; Funding; GPR3 gene; GTP-Binding Protein alpha Subunits, Gs; Heterogeneity; Lipids; Longevity; Maintenance; Modeling; Molecular Conformation; North Dakota; Optics; Pharmacology; Productivity; Radial; Regulation; Research; Research Personnel; Sampling; Scientist; Shapes; Signal Transduction; Supervision; Time; United States National Institutes of Health; Universities; Wages; college; heme a; instrument; instrumentation; macromolecule; protein protein interaction; service providers; student training; sugar; training opportunity

PROJECT SUMMARY/ABSTRACT Funds to purchase an Analytical Ultracentrifuge (AUC), model Beckman Couture Optima AUC, are being requested. This state-of-the-art instrument will expand the research capabilities of a core number of NIH funded investigators at North Dakota State University (NDSU). The AUC will allow characterization of macromolecules in solution to obtain quantitative details on the size, shape, heterogeneity, conformation, and assembly dynamics of these samples. This biophysical information provides critical information to understand the activation, inhibition, overall regulation, and general function of biological processes. The chosen instrument has both interference and absorbance optics allowing for the characterization of a broad range of sample types, including sugars and lipids that do not have characteristic absorbance coefficients. Currently, NDSU does not have this instrumentation and there is no AUC within a 200 mile radius. Therefore, users are currently relying on access to an AUC via long distance collaboration or for fee service providers. This can be inefficient and/or prohibitively expensive for investigators. The instrument will be housed in a core facility managed by a full time Ph.D. scientist, supported by NDSU and whose salary is not dependent on user fees. Thus, access and maintenance of the instrument will have direct supervision protecting the longevity of the instrument. Projects of immediate relevance that will be addressed involve 1) protein:protein interactions involved in bacterial cell surface signaling, 2) understanding agonist/antagonist influence on protein:protein interactions involve in a G-protein coupled receptor, 3) protein:protein interactions involved in autophagy regulation, and 4) assembly dynamics of a heme utilizing enzyme. Overall, our user group represents investigators in four departments across three colleges and the instrument will facilitate research in areas related to pharmacology to materials. The presence of an AUC at NDSU will greatly enhance the research directions of these and other projects of NIH relevance. It will significantly increase student training opportunities and research productivity of faculty.

项目总结/摘要 购买Beckman Couture Optima AUC型分析型超浓缩浓缩机（AUC）的资金如下： 被要求。这种最先进的仪器将扩大NIH核心研究能力 北达科他州州立大学（NDSU）资助的调查人员。AUC将允许表征 在溶液中的大分子，以获得定量的细节的大小，形状，异质性，构象， 这些样品的组装动力学。这些生物物理信息提供了重要的信息， 生物过程的激活、抑制、整体调节和一般功能。所选择的 仪器具有干涉和吸收光学器件，允许表征宽范围的 样品类型，包括不具有特征吸收系数的糖和脂质。目前， NDSU没有这种仪器，在200英里半径内没有AUC。因此，用户 目前依赖于通过远程协作或付费服务提供商访问AUC。这可以 效率低和/或昂贵得令人望而却步。该仪器将被安置在一个核心设施中， 由一位全职博士管理科学家，由国家发展科学研究所资助，其工资不依赖于使用费。 因此，仪器的访问和维护将受到直接监督， 仪器将被解决的直接相关的项目涉及1）蛋白质：蛋白质相互作用 参与细菌细胞表面信号传导，2）了解激动剂/拮抗剂对蛋白质的影响：蛋白质 G蛋白偶联受体中的相互作用，3）自噬中的蛋白质：蛋白质相互作用 调节，以及4）血红素利用酶的组装动力学。总的来说，我们的用户群代表了 调查人员在四个部门跨越三个学院和仪器将促进研究领域 与药理学和材料有关的。在国防科学研究所设立一个非盟委员会将大大加强研究工作， 这些项目和其他NIH相关项目的方向。它将大大增加学生的培训 机会和教师的研究生产力。