ShEEP Request for Confocal Microscope

ShEEP 请求共焦显微镜

• 批准号: 10179606
• 负责人: Machelle T. Pardue
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10179606
• 关键词: 12 year old; Alzheimer' s Disease; Area; Artificial Intelligence; Biological; Cells; Cerebrovascular system; Computer Workstations; Confocal Microscopy; Data; Diabetes Mellitus; Disease; Electrophysiology (science); Eye; Fibronectins; Funding; Future; Glaucoma; Goals; Health; Healthcare; Healthcare Systems; Image; Infection; Injury; Label; Light; Lung; Malignant Neoplasms; Microscope; Minor; Muscle; Neurons; Noise; Optics; Organ; Peripheral arterial disease; Photobleaching; Pulmonary Hypertension; Research; Research Personnel; Resolution; Sampling; Scanning; Scientist; Sheep; Signal Transduction; Skin; Source; Speed; Stroke; Structure; Subcellular structure; System; Time; Tissue imaging; Tissues; Traumatic Brain Injury; Veterans; base; bone; confocal imaging; high end computer; imaging software; improved; inflammatory disease of the intestine; innovation; lens; live cell imaging; pathogen; reconstruction; square foot; three dimensional structure; tool; ventilation

Abstract Microscopes were first invented in the 1590s and quickly became a fundamental part of research to visualize tissues and cells. In the 1940s, confocal microscopy was first introduced to increase optical resolution. The ability to image tissue at different depths enabled the reconstruction of three-dimensional structures within an object which greatly aided in understanding the relationship between different intracellular structures as well as the structural relationship between cells and neurons in healthy and diseased tissues. Due to this ability to interrogate biological structures in such detail, confocal microscopy has become an essential tool for scientists. Here, we request a new Nikon A1R HD25 MP ready confocal microscope which will replace our existing Olympus FV1000 confocal microscope that is over 12 years old (obtained with ShEEP funds in 2008). The Nikon A1R confocal has multiple advancements, including higher resolution, larger imaging area, faster acquisition speed, increased analytical throughput, more sophisticated imaging software that includes artificial intelligence components, live cell imaging and the ability to perform electrophysiology recordings simultaneously. Importantly, this new Nikon A1R confocal microscope can be easily upgraded to include multiphoton capabilities in the future, using the same footprint. Eighteen investigators at the Atlanta VA Healthcare System were identified that would be major and minor users of this microscope. They were eager to support this proposal with anticipation that upgrading to the Nikon A1R confocal microscopy would enhance their research by providing advanced imaging options. Thus, we propose the purchase of the Nikon A1R confocal microscopy system to support and accelerate the innovative research being performed by Atlanta VA investigators to improve veteran healthcare.

摘要 显微镜最早发明于16世纪90年代，并迅速成为可视化研究的基本组成部分。 组织和细胞。在20世纪40年代，共焦显微镜首次被引入以提高光学分辨率。的 在不同深度对组织成像的能力使得能够在一个 这极大地有助于理解不同细胞内结构之间的关系， 健康和患病组织中细胞和神经元之间的结构关系。由于这种能力， 由于能够如此详细地研究生物结构，共聚焦显微镜已成为科学家们必不可少的工具。 在这里，我们要求一个新的尼康A1R HD25 MP准备共聚焦显微镜，将取代我们现有的 Olympus FV 1000共聚焦显微镜已使用超过12年（2008年获得ShEEP基金）。的 尼康A1R共焦相机具有多项进步，包括更高的分辨率，更大的成像面积，更快的成像速度， 采集速度，增加的分析通量，更复杂的成像软件，包括人工 智能组件、活细胞成像和进行电生理记录的能力 同步重要的是，这款新的尼康A1R共聚焦显微镜可以轻松升级， 多光子能力在未来，使用相同的足迹。亚特兰大退伍军人管理局的18名调查员 医疗保健系统被确定为该显微镜的主要和次要用户。他们渴望 为了支持这一建议，预计升级到尼康A1R共聚焦显微镜将提高 他们的研究通过提供先进的成像选项。因此，我们建议购买尼康A1R 共聚焦显微镜系统，以支持和加速正在进行的创新研究亚特兰大弗吉尼亚州 调查人员改善退伍军人的医疗保健。