Neural circuits of vocal communication during social bonding

社会联系过程中声音交流的神经回路

• 批准号: 10191341
• 负责人: Morgan Gustison
• 金额: $ 10.64万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10191341
• 关键词: Advisory Committees; Affect; Anatomy; Animal Vocalization; Anterior; Area; Behavior; Behavioral; Biological Models; Botox; Brain; Brain imaging; CAV2 gene; Cognitive; Communication; Complex; Courtship; Data; Disease; Elements; Emotional; Foundations; Gene Expression; Goals; Human; Image; Immediate-Early Genes; Individual; Injections; Knowledge; Label; Laboratories; Laryngeal muscle structure; Larynx; Leadership; Learning; Light; Link; Literature; Loneliness; Maintenance; Mammals; Maps; Measures; Medial; Mediating; Mental disorders; Mentorship; Methods; Microscopy; Microtus; Midbrain structure; Modeling; Modernization; Motivation; Neuroanatomy; Neurons; Neurosciences; Pair Bond; Pathway interactions; Pattern; Phase; Population; Prefrontal Cortex; Primates; Process; Production; Rabies virus; Research; Research Proposals; Risk; Risk Factors; Rodent; Role; Shapes; Social Behavior; Social Interaction; Structure; Synapses; System; Taxes; Testing; Tracer; Training; Translating; Viral; Virus; Work; affiliative behavior; analysis pipeline; base; career; cingulate cortex; designer receptors exclusively activated by designer drugs; experimental study; gene induction; insight; mortality; neural circuit; neuromechanism; nonhuman primate; novel; prairie vole; preference; programs; relating to nervous system; response; skills; social; social attachment; social relationships; tool; vocalization

PROJECT SUMMARY Vocal communication is a fundamental behavior that humans and many other mammals use to cultivate social attachments. These social attachments are needed to protect against perceived loneliness, which is associated with an increased risk of mental disorders and physical disease. The long term goal of this research proposal is to uncover the unique role of vocal communication in building and maintaining social attachments, and to identify the neural circuits that link vocalization to bonding. Our laboratory has used whole-brain imaging of immediate- early gene (IEG) activity to map the neural systems active during pair-bonding in the monogamous prairie vole. This research implicates the medial prefrontal cortex (prelimbic, infralimbic, and anterior cingulate areas) as a key hub within the pair-bonding network. My work also shows that prairie voles are incredibly vocal throughout pair-bonding, with vocalization associated with affiliative interactions like courtship and proximity maintenance. We also find that vocalization rates are correlated with prefrontal IEG expression during bond formation. The proposed experiments will test the central hypothesis that prefrontal cortical regions modulate vocalizations that promote pair-bonding. The goal of the K99 phase research is to develop a foundational understanding of how vocal communication is involved in the pair-bonding process. In Aim 1, I will determine how inhibition of vocal production impacts affiliative interactions and whole-brain IEG induction during bond formation and maintenance. In the process, I will learn the systems neuroscience methods of iDISCO, light-sheet microscopy, and related analysis pipelines. In Aim 2a, I will map the prairie vole vocal circuit using trans-synaptic tracing and whole-brain imaging to determine where vocalization and bonding circuits overlap. This will deepen my training with whole- brain imaging and mammalian neuroanatomy, as well as train me in the viral mapping of neural circuits. To advance my managerial skills, my K99 training will also include formal, targeted training in leadership, project management, and mentorship, overseen by a diverse advisory committee. In the R00 phase, my research will test whether prefrontal regions promote vocalization during pair-bonding. In Aim 2b, I will map afferent and efferent projections of prefrontal regions to identify anatomical connections between the vocal and bonding circuits. In Aim 3, I will manipulate the anterior cingulate cortex and its descending projections to examine its influences on vocalization, bond formation, and bond maintenance. This research will provide insight on the neural substrates that link vocal behavior to social attachment. The training will allow me to build on my unique ethological background in animal vocalization with the modern tools of behavioral and systems neuroscience. The combined elements of this research proposal will lay a foundation for an independent career investigating the neural mechanisms through which communication supports healthy social connection.

项目摘要 人声交流是人类和许多其他哺乳动物培养社会的基本行为 附件。需要这些社会依恋来防止感知到的孤独感，这是相关的 患有精神障碍和身体疾病的风险增加。该研究建议的长期目标是 揭示声乐沟通在建立和维持社会依恋方面的独特作用，并确定 将发声与键合的神经回路。我们的实验室使用了直接的全脑成像 - 早期基因（IEG）活性以绘制一夫一妻制的草原vole成对束缚期间活跃的神经系统。 这项研究暗示内侧前额叶皮层（预临时，伸长膜和前扣带回区域） 配对网络中的关键集线器。我的作品还表明，草原田鼠在整个过程中都令人难以置信 配对键合，与求爱和接近性维护（例如求婚和接近性维护）相关的发声。 我们还发现，发声速率与键形成期间的前额叶IEG表达相关。这 拟议的实验将检验以下假设，即前额叶皮质区域调节发声 促进配对键。 K99阶段研究的目标是对如何建立对 配对过程涉及声音交流。在AIM 1中，我将确定如何抑制声音 生产会影响债券形成和维护期间的隶属关系和全脑IEG归纳。 在此过程中，我将学习IDISCO，灯页显微镜和相关的系统神经科学方法 分析管道。在AIM 2A中，我将使用反式突触跟踪和全脑图绘制草原vole声乐电路 成像以确定发声和粘结电路重叠的位置。这将加深我的培训 - 脑成像和哺乳动物神经解剖学，并在神经回路的病毒映射中训练我。到 提高我的管理技能，我的K99培训还将包括正式的，有针对性的领导力培训，项目 管理和指导，由多元化咨询委员会监督。在R00阶段，我的研究将 测试前额叶区域是否促进成对键入期间发声。在AIM 2B中，我将映射传入和 前额叶区域的传出预测，以识别声音与键合之间的解剖联系 电路。在AIM 3中，我将操纵前扣带回皮层及其下降预测以检查其 影响声音，债券形成和债券维护。这项研究将提供有关 将声音行为与社会依恋联系起来的神经底物。培训将使我以自己的独特 动物发声中的伦理背景具有现代行为和系统神经科学工具。 该研究建议的组合要素将为独立职业调查奠定基础 沟通支持健康社会联系的神经机制。