Neural circuits of vocal communication during social bonding

社会联系过程中声音交流的神经回路

• 批准号: 10191341
• 负责人: Morgan Gustison
• 金额: $ 10.64万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10191341
• 关键词: Advisory Committees; Affect; Anatomy; Animal Vocalization; Anterior; Area; Behavior; Behavioral; Biological Models; Botox; Brain; Brain imaging; CAV2 gene; Cognitive; Communication; Complex; Courtship; Data; Disease; Elements; Emotional; Foundations; Gene Expression; Goals; Human; Image; Immediate-Early Genes; Individual; Injections; Knowledge; Label; Laboratories; Laryngeal muscle structure; Larynx; Leadership; Learning; Light; Link; Literature; Loneliness; Maintenance; Mammals; Maps; Measures; Medial; Mediating; Mental disorders; Mentorship; Methods; Microscopy; Microtus; Midbrain structure; Modeling; Modernization; Motivation; Neuroanatomy; Neurons; Neurosciences; Pair Bond; Pathway interactions; Pattern; Phase; Population; Prefrontal Cortex; Primates; Process; Production; Rabies virus; Research; Research Proposals; Risk; Risk Factors; Rodent; Role; Shapes; Social Behavior; Social Interaction; Structure; Synapses; System; Taxes; Testing; Tracer; Training; Translating; Viral; Virus; Work; affiliative behavior; analysis pipeline; base; career; cingulate cortex; designer receptors exclusively activated by designer drugs; experimental study; gene induction; insight; mortality; neural circuit; neuromechanism; nonhuman primate; novel; prairie vole; preference; programs; relating to nervous system; response; skills; social; social attachment; social relationships; tool; vocalization

PROJECT SUMMARY Vocal communication is a fundamental behavior that humans and many other mammals use to cultivate social attachments. These social attachments are needed to protect against perceived loneliness, which is associated with an increased risk of mental disorders and physical disease. The long term goal of this research proposal is to uncover the unique role of vocal communication in building and maintaining social attachments, and to identify the neural circuits that link vocalization to bonding. Our laboratory has used whole-brain imaging of immediate- early gene (IEG) activity to map the neural systems active during pair-bonding in the monogamous prairie vole. This research implicates the medial prefrontal cortex (prelimbic, infralimbic, and anterior cingulate areas) as a key hub within the pair-bonding network. My work also shows that prairie voles are incredibly vocal throughout pair-bonding, with vocalization associated with affiliative interactions like courtship and proximity maintenance. We also find that vocalization rates are correlated with prefrontal IEG expression during bond formation. The proposed experiments will test the central hypothesis that prefrontal cortical regions modulate vocalizations that promote pair-bonding. The goal of the K99 phase research is to develop a foundational understanding of how vocal communication is involved in the pair-bonding process. In Aim 1, I will determine how inhibition of vocal production impacts affiliative interactions and whole-brain IEG induction during bond formation and maintenance. In the process, I will learn the systems neuroscience methods of iDISCO, light-sheet microscopy, and related analysis pipelines. In Aim 2a, I will map the prairie vole vocal circuit using trans-synaptic tracing and whole-brain imaging to determine where vocalization and bonding circuits overlap. This will deepen my training with whole- brain imaging and mammalian neuroanatomy, as well as train me in the viral mapping of neural circuits. To advance my managerial skills, my K99 training will also include formal, targeted training in leadership, project management, and mentorship, overseen by a diverse advisory committee. In the R00 phase, my research will test whether prefrontal regions promote vocalization during pair-bonding. In Aim 2b, I will map afferent and efferent projections of prefrontal regions to identify anatomical connections between the vocal and bonding circuits. In Aim 3, I will manipulate the anterior cingulate cortex and its descending projections to examine its influences on vocalization, bond formation, and bond maintenance. This research will provide insight on the neural substrates that link vocal behavior to social attachment. The training will allow me to build on my unique ethological background in animal vocalization with the modern tools of behavioral and systems neuroscience. The combined elements of this research proposal will lay a foundation for an independent career investigating the neural mechanisms through which communication supports healthy social connection.

项目摘要 声音交流是人类和许多其他哺乳动物用来培养社会性的基本行为 附件的权限.这些社会依恋是防止孤独感的必要条件， 患精神疾病和身体疾病的风险增加。这项研究计划的长远目标是 揭示声音沟通在建立和维持社会依恋中的独特作用，并确定 连接发声和结合的神经回路我们的实验室使用全脑成像技术- 早期基因（IEG）的活动，以映射在一夫一妻制草原田鼠配对过程中活跃的神经系统。 这项研究暗示内侧前额叶皮层（前边缘，边缘下，前扣带区）作为一个 配对绑定网络中的关键枢纽。我的研究还表明，草原田鼠在整个过程中 配对结合，与像求爱和接近维护附属互动相关的发声。 我们还发现，发声率与前额叶IEG表达在债券形成。的 提出的实验将检验中心假设，即前额叶皮层区域调节发声， 促进配对结合。K99阶段研究的目标是对如何 语音交流涉及配对结合过程。在目标1中，我将确定如何抑制声乐 生产影响亲和的相互作用和全脑IEG诱导过程中的债券形成和维护。 在这个过程中，我将学习iDISCO的系统神经科学方法，光片显微镜，以及相关的 分析管道。在目标2a中，我将使用跨突触追踪和全脑定位绘制草原田鼠的发声回路。 成像来确定发声和结合电路的重叠部分。这将加深我的训练与整个- 大脑成像和哺乳动物神经解剖学，以及训练我神经回路的病毒映射。到 为了提高我的管理技能，我的K99培训还将包括领导力、项目 管理和指导，由一个多元化的咨询委员会监督。在R 00阶段，我的研究将 测试前额叶区域是否在配对过程中促进发声。在目标2b中，我将映射传入和 前额叶区域的传出投射，以识别发声和结合之间的解剖学联系 电路.在目标3中，我将操纵前扣带皮层及其下行投射，以检查其 影响发声、键形成和键维持。这项研究将提供洞察力 将发声行为与社会依恋联系起来的神经基质。培训将使我能够建立我独特的 动物发声的行为学背景与行为和系统神经科学的现代工具。 本研究建议的综合要素将为独立的职业调查奠定基础 神经机制，通过它的沟通支持健康的社会联系。