Neural circuits of vocal communication during social bonding

社会联系过程中声音交流的神经回路

• 批准号: 10191341
• 负责人: Morgan Gustison
• 金额: $ 10.64万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10191341
• 关键词: Advisory Committees; Affect; Anatomy; Animal Vocalization; Anterior; Area; Behavior; Behavioral; Biological Models; Botox; Brain; Brain imaging; CAV2 gene; Cognitive; Communication; Complex; Courtship; Data; Disease; Elements; Emotional; Foundations; Gene Expression; Goals; Human; Image; Immediate-Early Genes; Individual; Injections; Knowledge; Label; Laboratories; Laryngeal muscle structure; Larynx; Leadership; Learning; Light; Link; Literature; Loneliness; Maintenance; Mammals; Maps; Measures; Medial; Mediating; Mental disorders; Mentorship; Methods; Microscopy; Microtus; Midbrain structure; Modeling; Modernization; Motivation; Neuroanatomy; Neurons; Neurosciences; Pair Bond; Pathway interactions; Pattern; Phase; Population; Prefrontal Cortex; Primates; Process; Production; Rabies virus; Research; Research Proposals; Risk; Risk Factors; Rodent; Role; Shapes; Social Behavior; Social Interaction; Structure; Synapses; System; Taxes; Testing; Tracer; Training; Translating; Viral; Virus; Work; affiliative behavior; analysis pipeline; base; career; cingulate cortex; designer receptors exclusively activated by designer drugs; experimental study; gene induction; insight; mortality; neural circuit; neuromechanism; nonhuman primate; novel; prairie vole; preference; programs; relating to nervous system; response; skills; social; social attachment; social relationships; tool; vocalization

PROJECT SUMMARY Vocal communication is a fundamental behavior that humans and many other mammals use to cultivate social attachments. These social attachments are needed to protect against perceived loneliness, which is associated with an increased risk of mental disorders and physical disease. The long term goal of this research proposal is to uncover the unique role of vocal communication in building and maintaining social attachments, and to identify the neural circuits that link vocalization to bonding. Our laboratory has used whole-brain imaging of immediate- early gene (IEG) activity to map the neural systems active during pair-bonding in the monogamous prairie vole. This research implicates the medial prefrontal cortex (prelimbic, infralimbic, and anterior cingulate areas) as a key hub within the pair-bonding network. My work also shows that prairie voles are incredibly vocal throughout pair-bonding, with vocalization associated with affiliative interactions like courtship and proximity maintenance. We also find that vocalization rates are correlated with prefrontal IEG expression during bond formation. The proposed experiments will test the central hypothesis that prefrontal cortical regions modulate vocalizations that promote pair-bonding. The goal of the K99 phase research is to develop a foundational understanding of how vocal communication is involved in the pair-bonding process. In Aim 1, I will determine how inhibition of vocal production impacts affiliative interactions and whole-brain IEG induction during bond formation and maintenance. In the process, I will learn the systems neuroscience methods of iDISCO, light-sheet microscopy, and related analysis pipelines. In Aim 2a, I will map the prairie vole vocal circuit using trans-synaptic tracing and whole-brain imaging to determine where vocalization and bonding circuits overlap. This will deepen my training with whole- brain imaging and mammalian neuroanatomy, as well as train me in the viral mapping of neural circuits. To advance my managerial skills, my K99 training will also include formal, targeted training in leadership, project management, and mentorship, overseen by a diverse advisory committee. In the R00 phase, my research will test whether prefrontal regions promote vocalization during pair-bonding. In Aim 2b, I will map afferent and efferent projections of prefrontal regions to identify anatomical connections between the vocal and bonding circuits. In Aim 3, I will manipulate the anterior cingulate cortex and its descending projections to examine its influences on vocalization, bond formation, and bond maintenance. This research will provide insight on the neural substrates that link vocal behavior to social attachment. The training will allow me to build on my unique ethological background in animal vocalization with the modern tools of behavioral and systems neuroscience. The combined elements of this research proposal will lay a foundation for an independent career investigating the neural mechanisms through which communication supports healthy social connection.

项目总结 语音交流是人类和许多其他哺乳动物用来培养社交能力的一种基本行为 附属品。这些社交依恋是需要的，以防止感知到的孤独，这与 精神障碍和身体疾病的风险增加。这项研究计划的长期目标是 揭示声音交流在建立和维持社会依恋中的独特作用，并确定 将发声与结合联系起来的神经回路。我们的实验室使用了全脑成像技术-- 早期基因(IEG)活性，以定位在一夫一妻制草原田鼠配对期间活跃的神经系统。 这项研究表明，内侧前额叶皮质(前额叶、下缘和前扣带回)是 配对网络中的关键集线器。我的研究还表明，草原田鼠在整个过程中都发出令人难以置信的声音 配对结合，发声与求爱和维持亲密关系等附属互动有关。 我们还发现，发声速率与前额叶IEG在债券形成过程中的表达有关。这个 拟议的实验将检验这一中心假设，即前额叶皮质区域调制发声 促进结对。K99阶段研究的目标是建立对以下方面的基本理解 配对过程中涉及到语音交流。在目标1中，我将确定如何抑制发声 在键的形成和维持过程中，生产影响从属相互作用和全脑IEG诱导。 在这个过程中，我将学习IDISCO的系统神经科学方法，光学显微镜，以及相关的 分析管道。在目标2a中，我将使用跨突触追踪和全脑绘制草原田鼠发声回路图 成像以确定发声和键合电路重叠的位置。这将深化我与全员的训练- 大脑成像和哺乳动物神经解剖学，以及在神经回路的病毒映射方面的培训。至 提高我的管理技能，我的K99培训还将包括正式的、有针对性的领导力培训、项目 管理和指导，由一个多元化的咨询委员会监督。在R00阶段，我的研究将 测试前额叶区域在配对时是否促进发声。在目标2b中，我将映射传入和 前额叶区域的传出投射以确定发声和粘连之间的解剖联系 电路。在目标3中，我将操纵前扣带回皮质及其下行投射，以检查其 对发声、键形成和键保持的影响。这项研究将为我们提供关于 将发声行为与社会依恋联系起来的神经基础。这次培训将使我在我独特的基础上 动物发声的行为学背景与现代行为学和系统神经科学的工具。 这项研究提案的综合要素将为独立的职业调查奠定基础 沟通支持健康社会关系的神经机制。