Rapid Characterization of the Vaginal Microbiome/Virome in Pregnant Women

孕妇阴道微生物组/病毒组的快速表征

• 批准号: 10190582
• 负责人: Kristine M. Wylie
• 金额: $ 7.88万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-03-08 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10190582
• 关键词: 37 weeks gestation; Affect; Bacteria; Biological Assay; Biological Markers; Birth; Characteristics; Clinical; Clinical Research; Communities; Consumption; Cultured Cells; DNA; Data; Data Set; Detection; Development; Disease; Eukaryota; Fibrinogen; First Pregnancy Trimester; Follow-Up Studies; Foundations; Future; Gardnerella vaginalis; High-Throughput Nucleotide Sequencing; Individual; Microbe; Neonatal Mortality; Nucleic Acids; Papillomavirus; Pregnant Women; Premature Birth; Publishing; RNA; Resources; Risk; Sampling; Sensitivity and Specificity; Swab; Term Birth; Testing; Time; Trichomonas vaginalis; United States; Vagina; Viral; Virus; Work; assay development; bacterial community; base; cost; cost effective; design; human female; innovation; interest; metagenomic sequencing; microbial; microbial community; microbial signature; microbiome analysis; microbiome research; multiplex detection; nano-string; neonatal morbidity; prevent; reproductive tract; success; vaginal microbiome; vaginal microbiota; virome

ABSTRACT Preterm birth (PTB), delivery occurring before 37 weeks of gestation, affects approximately 10% of births in the United States each year and is the primary cause of neonatal morbidities and mortality. Because our understanding of the causes of PTB is incomplete, we are limited in our ability to predict or prevent PTB. In our previous studies, we used high-throughput sequencing to reveal characteristics of viral and bacterial communities in the vagina are associated with PTB. These data suggest that characteristics of the vaginal microbiome may serve as biomarkers for PTB, although more studies are required. While sequencing assays have been useful in beginning to identify microbial signatures associated with PTB, the incorporation of these assays into large-scale follow up studies is limited by several factors: (i) sequencing assays are in many cases qualitative, requiring subsequent assays to quantitate the microbes, thereby depleting sample material; (ii) sequencing and qPCR assays are time-consuming, requiring weeks to obtain complete data sets from each sample; and (iii) sequencing assays are relatively expensive despite many innovations that have reduced costs. Based on our increased understanding of vaginal microbial community features associated with PTB, we presently aim to develop a single, multiplexed, targeted assay that will allow us to capture the essential features we find in sequencing assays, but it will do so more quantitatively, rapidly, and cost-effectively than sequencing. The development of this assay will allow us (and others who study the microbiome of the human female reproductive tract) to maximize resource utilization in future studies by replacing multiple sequencing and PCR tests with a single, quantitative, rapid, easy to use, less expensive assay. Furthermore, this assay may serve as a foundation for future clinical studies aimed at defining risk for PTB in pregnant women

摘要 早产（PTB），即在妊娠37周之前分娩，影响了美国约10%的分娩。 是美国每年新生儿发病和死亡的主要原因。因为我们的 由于对肺结核病因的了解不完全，我们预测或预防肺结核的能力有限。在我们 在以前的研究中，我们使用高通量测序来揭示病毒和细菌的特征， 阴道中的细菌群落与PTB有关。这些数据表明，阴道的特征 微生物组可以作为PTB的生物标志物，但需要更多的研究。当测序分析 在开始鉴定与PTB相关的微生物特征时是有用的， 将测序分析用于大规模的后续研究受到几个因素的限制：（i）测序分析在许多情况下 定性的，需要随后的测定来定量微生物，从而耗尽样品材料;（ii） 测序和qPCR分析是耗时的，需要数周时间才能获得每个分析的完整数据集。 样品;以及（iii）测序测定相对昂贵，尽管许多创新降低了成本。 基于我们对与PTB相关的阴道微生物群落特征的了解，我们 目前的目标是开发一个单一的，多重的，有针对性的测定，使我们能够捕捉的基本特征， 我们在测序分析中发现，但它将比测序更定量，更快速，更经济。 这项检测的发展将使我们（以及其他研究人类女性微生物组的人） 生殖道），以最大限度地利用资源，在未来的研究中，取代多重测序和PCR 使用单一、定量、快速、易于使用、成本较低的检测方法进行检测。此外，该测定可用作 为未来旨在确定孕妇PTB风险的临床研究奠定基础