The Metagenomics Education Partnership: Harnessing the Power of Microbial Genome Sequencing and Big Data with High School Students and Teachers.

宏基因组学教育合作伙伴关系：与高中生和教师一起利用微生物基因组测序和大数据的力量。

• 批准号: 10190973
• 负责人: STEPHEN T KOURY
• 金额: $ 26.07万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-15 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10190973
• 关键词: Area Health Education Centers; Award; Big Data; Bioinformatics; Biological Sciences; Biotechnology; Buffaloes; Clinical; Collaborations; Communities; Computer software; County; DNA; DNA sequencing; Data; Data Set; Disadvantaged; Education; Education Projects; Educational workshop; Evaluation; Event; Faculty; Future; Future Generations; Future Teacher; Genbank; Generations; Genetic; Genome; Genomic DNA; Genomics; Goals; Grant; Health; Healthcare; High School Student; Intervention; Laboratories; Learning Skill; Link; Metagenomics; Methods; Microbial Genome Sequencing; Microbiology; Mission; New York; Occupations; Organism; Output; Ownership; Participant; Performance; Population Scientist Supplement (R25); Preparation; Procedures; Research; Research Project Grants; Resources; Rivers; Role; Running; Sampling; Schools; Science; Sequence Analysis; Stream; Student recruitment; Students; System; Teacher Professional Development; Technology; Third Generation Sequencing; Time; Training; Underrepresented Populations; United States National Institutes of Health; Universities; Water; Workforce Development; career; citizen science; college; design; disadvantaged student; empowered; experience; high school; interest; medical schools; microbial; microbial community; microbial composition; microbial genome; microbial genomics; nanopore; novel; programs; recruit; routine Bacterial stain; school district; science education; sequencing platform; symposium; teacher; tool; water sampling

The Metagenomics Education Partnership: Harnessing the Power of Microbial Genome Sequencing and Big Data with High School Students and Teachers will further an ongoing partnership between the Department of Biotechnical and Clinical Laboratory Sciences (BCLS) of the University at Buffalo Jacobs School of Medicine and Biomedical Sciences, the New York State Area Health Education Center System (NYSAHEC), the Center of Excellence in Bioinformatics & Life Sciences at the University at Buffalo and the Buffalo Niagara Waterkeeper (BNW) with underserved/disadvantaged schools across a 14-county region of Western New York. The proposal activities will 1) develop and strengthen partnerships between local high schools, colleges, biotechnology companies, and local not-for profit organizations and serve as a pipeline for recruiting students to scientific and health-related careers, with an emphasis on those from underrepresented groups; 2) utilize community-linked citizen science involving metagenomic analyses of water samples in Western New York with underserved high school students, empowering them to assist in safeguarding local water resources for present and future generations; and 3) allow high school students and teachers to sequence and analyze a microbial genome, supporting their explorations of Big Data, STEM and health-related careers related to genomics. Proposal participants will be among the first high school students and teachers to use third generation Oxford Nanopore MinION sequencing technology, giving them first-hand experience with the preparation of genomic DNA samples for sequencing, in school DNA sequencing, tools for determining the makeup of microbial communities from sequencing data, and the construction of a microbial genome through the compilation of a large number of overlapping long MinION sequence reads. The project will include a week-long teacher professional development covering hands on experience with all aspects of project activities in the summer, followed by three different day-long refresher training sessions for teachers during the school year. Students will perform identical project activities during the academic year under the guidance of their teachers and faculty from the partnership. Year one of the proposal will be formative, involving two schools and three teachers along with a total of 30 students. Years 2-5 will have ten intervention teachers and 100 students per year take part, who will be compared with a total of 30 teachers and 300 students as matched controls in years 2-4 as part of the evaluation plan. Students and teachers will present data at an annual capstone event each spring. Their data on waterway quality will in addition be shared and publicized by BNW, and their microbial genome sequencing data will ultimately be shared on GenBank. The authentic research experiences for students involving new DNA sequencing technology and big data manipulations will increase their interest, engagement and future performance in STEM, and positively influence their long-term STEM and health-related career goals.

宏基因组学教育合作伙伴关系：利用微生物基因组测序和大基因组的力量 与高中生和教师的数据将进一步促进教育部之间的持续合作关系 布法罗大学雅各布斯医学院生物技术和临床实验室科学 (BCLS) 和生物医学科学，纽约州地区健康教育中心系统（NYSAHEC），该中心 布法罗大学和布法罗尼亚加拉水卫士生物信息学和生命科学卓越奖 (BNW) 与纽约州西部 14 个县地区的服务不足/弱势学校合作。提案 活动将 1) 发展和加强当地高中、大学、生物技术之间的伙伴关系 公司和当地非营利组织，并作为招募学生进入科学和技术领域的渠道 与健康相关的职业，重点是来自代表性不足群体的职业； 2）利用社区链接 公民科学涉及对纽约州西部服务水平低下的水样进行宏基因组分析 使学生能够协助保护当前和未来的当地水资源 几代人； 3) 允许高中生和教师对微生物基因组进行测序和分析， 支持他们对大数据、STEM 以及与基因组学相关的健康相关职业的探索。提议 参与者将成为第一批使用第三代 Oxford Nanopore 的高中生和教师 MinION 测序技术，为他们提供基因组 DNA 样本制备的第一手经验 用于测序，在学校 DNA 测序中，用于确定微生物群落组成的工具 测序数据，并通过汇编大量的微生物基因组来构建 重叠长 MinION 序列读数。该项目将包括为期一周的专业教师 开发涵盖夏季项目活动各个方面的实践经验，然后是三个 学年期间为教师提供不同的为期一天的进修培训课程。学生将表演相同的 学年期间在合作伙伴的教师和教员的指导下开展项目活动。 该提案的第一年将是形成期，涉及两所学校和三名教师以及总共 30 名教师 学生。 2-5 年级每年将有 10 名干预教师和 100 名学生参与，他们将 作为评估的一部分，2-4 年总共 30 名教师和 300 名学生作为匹配对照 计划。学生和教师将在每年春天的年度顶点活动中展示数据。他们的水路数据 此外，BNW 还将共享和公布质量，他们的微生物基因组测序数据将 最终在 GenBank 上共享。为学生提供涉及新 DNA 的真实研究体验 测序技术和大数据操作将增加他们的兴趣、参与度和未来 STEM 方面的表现，并对他们的长期 STEM 和健康相关职业目标产生积极影响。