The Metagenomics Education Partnership: Harnessing the Power of Microbial Genome Sequencing and Big Data with High School Students and Teachers.

宏基因组学教育合作伙伴关系：与高中生和教师一起利用微生物基因组测序和大数据的力量。

• 批准号: 10190973
• 负责人: STEPHEN T KOURY
• 金额: $ 26.07万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-15 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10190973
• 关键词: Area Health Education Centers; Award; Big Data; Bioinformatics; Biological Sciences; Biotechnology; Buffaloes; Clinical; Collaborations; Communities; Computer software; County; DNA; DNA sequencing; Data; Data Set; Disadvantaged; Education; Education Projects; Educational workshop; Evaluation; Event; Faculty; Future; Future Generations; Future Teacher; Genbank; Generations; Genetic; Genome; Genomic DNA; Genomics; Goals; Grant; Health; Healthcare; High School Student; Intervention; Laboratories; Learning Skill; Link; Metagenomics; Methods; Microbial Genome Sequencing; Microbiology; Mission; New York; Occupations; Organism; Output; Ownership; Participant; Performance; Population Scientist Supplement (R25); Preparation; Procedures; Research; Research Project Grants; Resources; Rivers; Role; Running; Sampling; Schools; Science; Sequence Analysis; Stream; Student recruitment; Students; System; Teacher Professional Development; Technology; Third Generation Sequencing; Time; Training; Underrepresented Populations; United States National Institutes of Health; Universities; Water; Workforce Development; career; citizen science; college; design; disadvantaged student; empowered; experience; high school; interest; medical schools; microbial; microbial community; microbial composition; microbial genome; microbial genomics; nanopore; novel; programs; recruit; routine Bacterial stain; school district; science education; sequencing platform; symposium; teacher; tool; water sampling

The Metagenomics Education Partnership: Harnessing the Power of Microbial Genome Sequencing and Big Data with High School Students and Teachers will further an ongoing partnership between the Department of Biotechnical and Clinical Laboratory Sciences (BCLS) of the University at Buffalo Jacobs School of Medicine and Biomedical Sciences, the New York State Area Health Education Center System (NYSAHEC), the Center of Excellence in Bioinformatics & Life Sciences at the University at Buffalo and the Buffalo Niagara Waterkeeper (BNW) with underserved/disadvantaged schools across a 14-county region of Western New York. The proposal activities will 1) develop and strengthen partnerships between local high schools, colleges, biotechnology companies, and local not-for profit organizations and serve as a pipeline for recruiting students to scientific and health-related careers, with an emphasis on those from underrepresented groups; 2) utilize community-linked citizen science involving metagenomic analyses of water samples in Western New York with underserved high school students, empowering them to assist in safeguarding local water resources for present and future generations; and 3) allow high school students and teachers to sequence and analyze a microbial genome, supporting their explorations of Big Data, STEM and health-related careers related to genomics. Proposal participants will be among the first high school students and teachers to use third generation Oxford Nanopore MinION sequencing technology, giving them first-hand experience with the preparation of genomic DNA samples for sequencing, in school DNA sequencing, tools for determining the makeup of microbial communities from sequencing data, and the construction of a microbial genome through the compilation of a large number of overlapping long MinION sequence reads. The project will include a week-long teacher professional development covering hands on experience with all aspects of project activities in the summer, followed by three different day-long refresher training sessions for teachers during the school year. Students will perform identical project activities during the academic year under the guidance of their teachers and faculty from the partnership. Year one of the proposal will be formative, involving two schools and three teachers along with a total of 30 students. Years 2-5 will have ten intervention teachers and 100 students per year take part, who will be compared with a total of 30 teachers and 300 students as matched controls in years 2-4 as part of the evaluation plan. Students and teachers will present data at an annual capstone event each spring. Their data on waterway quality will in addition be shared and publicized by BNW, and their microbial genome sequencing data will ultimately be shared on GenBank. The authentic research experiences for students involving new DNA sequencing technology and big data manipulations will increase their interest, engagement and future performance in STEM, and positively influence their long-term STEM and health-related career goals.

元基因组学教育伙伴关系：利用微生物基因组测序和BIG的力量 与高中生和教师的数据将进一步促进教育部之间正在进行的伙伴关系 布法罗-雅各布斯大学医学院生物技术和临床检验科学 和生物医学科学，纽约州地区健康教育中心系统(NYSAHEC)，该中心 布法罗大学和水牛城尼亚加拉保水员生物信息学和生命科学杰出奖 (BNW)在纽约西部14个县地区拥有服务不足/处境不利的学校。这项建议 活动将1)发展和加强当地高中、大学、生物技术 公司和当地的非营利性组织，并作为招收学生进入科学和 与健康相关的职业，重点是那些代表人数不足的群体；2)利用与社区有联系的职业 涉及对纽约西部未得到充分服务的高水样进行元基因组分析的公民科学 学校学生，使他们有能力协助保护当前和未来的当地水资源 以及3)允许高中生和老师对微生物基因组进行排序和分析， 支持他们探索与基因组学相关的大数据、STEM和健康相关职业。建议书 参与者将是第一批使用第三代牛津纳米孔的高中生和教师 Minion测序技术，让他们获得制备基因组DNA样本的第一手经验 在学校DNA测序中，用于确定微生物群落组成的工具 测序数据，并通过汇编大量的微生物基因组构建 重叠的长附属物序列阅读。该项目将包括一名为期一周的专业教师 开发，包括夏季项目活动的所有方面的实践经验，随后是三个 在学年期间为教师提供不同的为期一天的进修培训课程。学生们的表现将完全相同 在本学年项目活动的指导下，由教师和教职员工结成伙伴关系。 第一年的建议将是形成性的，涉及两所学校和三名教师，以及总共30名 学生们。2-5年级将有10名干预教师和每年100名学生参加，他们将是 相比之下，作为评估的一部分，2-4年级共有30名教师和300名学生作为对照 计划。每年春天，学生和教师将在一年一度的顶峰活动上展示数据。他们在水路上的数据 此外，BNW还将分享和公布质量，他们的微生物基因组测序数据将 最终在GenBank上共享。涉及新DNA的学生的真实研究体验 测序技术和大数据操作将提高他们的兴趣、参与度和未来 在STEM的表现，并对他们的长期STEM和与健康相关的职业目标产生积极影响。