The Metagenomics Education Partnership: Harnessing the Power of Microbial Genome Sequencing and Big Data with High School Students and Teachers.

宏基因组学教育合作伙伴关系：与高中生和教师一起利用微生物基因组测序和大数据的力量。

• 批准号: 10629348
• 负责人: STEPHEN T KOURY
• 金额: $ 25.28万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-15 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10629348
• 关键词: Area Health Education Centers; Award; Big Data; Bioinformatics; Biological; Biological Sciences; Biotechnology; Clinical; Collaborations; Communities; Computer software; County; DNA; DNA sequencing; Data; Data Set; Disadvantaged; Education; Education Projects; Educational workshop; Evaluation; Event; Faculty; Future; Future Generations; Genbank; Generations; Genetic; Genome; Genomic DNA; Genomics; Goals; Grant; Health; Healthcare; High School Student; Intervention; Laboratories; Learning Skill; Link; Metagenomics; Methods; Microbial Genome Sequencing; Microbiology; Mission; New York; Occupations; Organism; Output; Ownership; Participant; Performance; Population Scientist Supplement (R25); Preparation; Procedures; Research; Research Project Grants; Resources; Role; Running; Sampling; Schools; Science; Sequence Analysis; Stream; Student recruitment; Students; System; Teacher Professional Development; Technology; Third Generation Sequencing; Time; Training; Underrepresented Populations; United States National Institutes of Health; Universities; Water; Workforce Development; career; citizen science; college; design; disadvantaged student; empowerment; experience; high school; interest; medical schools; microbial; microbial community; microbial composition; microbial genome; microbial genomics; nanopore; novel; programs; recruit; routine Bacterial stain; school district; science education; sequencing platform; symposium; teacher; tool; water sampling

The Metagenomics Education Partnership: Harnessing the Power of Microbial Genome Sequencing and Big Data with High School Students and Teachers will further an ongoing partnership between the Department of Biotechnical and Clinical Laboratory Sciences (BCLS) of the University at Buffalo Jacobs School of Medicine and Biomedical Sciences, the New York State Area Health Education Center System (NYSAHEC), the Center of Excellence in Bioinformatics & Life Sciences at the University at Buffalo and the Buffalo Niagara Waterkeeper (BNW) with underserved/disadvantaged schools across a 14-county region of Western New York. The proposal activities will 1) develop and strengthen partnerships between local high schools, colleges, biotechnology companies, and local not-for profit organizations and serve as a pipeline for recruiting students to scientific and health-related careers, with an emphasis on those from underrepresented groups; 2) utilize community-linked citizen science involving metagenomic analyses of water samples in Western New York with underserved high school students, empowering them to assist in safeguarding local water resources for present and future generations; and 3) allow high school students and teachers to sequence and analyze a microbial genome, supporting their explorations of Big Data, STEM and health-related careers related to genomics. Proposal participants will be among the first high school students and teachers to use third generation Oxford Nanopore MinION sequencing technology, giving them first-hand experience with the preparation of genomic DNA samples for sequencing, in school DNA sequencing, tools for determining the makeup of microbial communities from sequencing data, and the construction of a microbial genome through the compilation of a large number of overlapping long MinION sequence reads. The project will include a week-long teacher professional development covering hands on experience with all aspects of project activities in the summer, followed by three different day-long refresher training sessions for teachers during the school year. Students will perform identical project activities during the academic year under the guidance of their teachers and faculty from the partnership. Year one of the proposal will be formative, involving two schools and three teachers along with a total of 30 students. Years 2-5 will have ten intervention teachers and 100 students per year take part, who will be compared with a total of 30 teachers and 300 students as matched controls in years 2-4 as part of the evaluation plan. Students and teachers will present data at an annual capstone event each spring. Their data on waterway quality will in addition be shared and publicized by BNW, and their microbial genome sequencing data will ultimately be shared on GenBank. The authentic research experiences for students involving new DNA sequencing technology and big data manipulations will increase their interest, engagement and future performance in STEM, and positively influence their long-term STEM and health-related career goals.

宏基因组学教育合作伙伴关系：利用微生物基因组测序和大 与高中学生和教师的数据将进一步促进教育部之间正在进行的伙伴关系， 布法罗雅各布斯大学医学院生物技术和临床实验室科学（BCLS） 和生物医学科学，纽约州地区健康教育中心系统（NYSAHEC），该中心 布法罗大学和布法罗尼亚加拉大学的生物信息学和生命科学卓越奖 (BNW)与纽约西部14个县的服务不足/弱势学校合作。该提案 活动将1）发展和加强当地高中，大学，生物技术 公司，和当地的非营利组织，并作为一个管道，招募学生的科学和 与健康有关的职业，重点是那些来自代表性不足的群体; 2）利用与社区有关的 公民科学涉及纽约西部水样的宏基因组分析， 学校的学生，使他们能够协助保护当地的水资源，为现在和未来 代;和3）允许高中学生和教师测序和分析微生物基因组， 支持他们探索大数据，STEM和与基因组学相关的健康相关职业。提案 参与者将成为第一批使用第三代牛津纳米孔的高中学生和教师。 MinION测序技术，为他们提供基因组DNA样品制备的第一手经验 测序，在学校DNA测序，用于确定微生物群落组成的工具， 测序数据，并通过编译大量的微生物基因组， 重叠的长MinION序列读数。该项目将包括为期一周的教师专业 发展涵盖了夏季项目活动各个方面的实践经验，然后是三个 在学年中为教师举办为期一天的进修培训班。学生将进行相同的 项目活动在本学年期间在其教师的指导下与合作伙伴教员共同进行。 第一年的提案将是形成性的，涉及两所学校和三名教师沿着，总共30名 学生2-5年级将有10名干预教师和100名学生每年参加，他们将 作为评估的一部分，在第2-4年，共有30名教师和300名学生作为匹配的对照组， 计划学生和教师将在每年春天的年度高峰活动中展示数据。他们的水路数据 此外，BNW还将分享和公布质量，他们的微生物基因组测序数据将 最终在GenBank上共享。为涉及新DNA的学生提供真实的研究体验 测序技术和大数据操作将增加他们的兴趣，参与和未来 在STEM的表现，并积极影响他们的长期STEM和健康相关的职业目标。