Core 1: Non-human primate core

摘要-非人灵长类(NHP)核心(核心1，领导者：Guido Silvestri博士，Yerkes National 埃默里大学灵长类研究中心) 最合适的动物模型来定义体液和细胞免疫的起源、动力学和影响- 婴儿出生后HIV-1感染后病毒反弹的策略和治疗 恒河猴免疫缺陷病毒(SIV)/猴-人免疫缺陷病毒(SIV)感染。 事实上，这个模型特别适合我们提议的研究，因为儿科出生后的模型 传播已被开发并用于定义婴儿HIV-1感染的发病机制和 预防策略。然而，NHP研究需要广泛的基础设施和独特的专业知识。因此，我们 该计划的非人灵长类(NHP)核心(核心1)将协调和实施所有NHP实验 由该方案的两个项目提出：项目1：“婴儿病毒反弹的起源和预测因素”，(PI：Dr. Emory大学的Ann Chahroudi)和项目2：基于免疫干预对病毒反弹的影响 经口服感染希沃克病毒的幼猴“(PI：杜克大学的吉纳维芙·福达博士)。核心将支持 通过以下具体目标实施方案：目标1--组织、协调和开展婴儿SHV研究 储存和反弹；目标2-口服SHV感染的抗逆转录病毒治疗(ART)的管理和监测 婴儿猴；和AIM 3-对婴儿注射多克隆抗体进行药代动力学分析 确定猕猴治疗婴幼儿新城疫病毒感染的剂量和给药间隔。由Dr。 Guido Silvestri，NHP核心将确保以下内容对于成功完成这些任务至关重要 项目：1)遵守与国家方案研究有关的监管程序；2)协调和 执行所有与动物有关的程序，包括为婴儿提供住房、接种疫苗、养育 婴儿，抗逆转录病毒治疗，以及每周病毒载量测量和每月血液样本采集 计数；3)适当储存和/或将样品运往项目和岩心；4)维护 临床健康参数数据库。西尔维斯特里博士将确保所有NHP支持的研究工作都是严格的 将满足研究项目不断变化的需求。作为本产品的一个组成部分 计划，NHP核心将提供关键支持，以实现理解来源、 免疫干预对出生后感染婴儿HIV-1反弹的动力学和影响。

ABSTRACT – Nonhuman Primate (NHP) Core (Core 1, Leader: Dr. Guido Silvestri, Yerkes National Primate Research Center, Emory University) The most appropriate animal model to define the origin, kinetics, and impact of humoral and cellular immune- based strategies on viral rebound in infants following postnatal HIV-1 infection and treatment is simian immunodeficiency virus (SIV)/simian-human immunodeficiency virus (SHIV) infection of rhesus macaques. Indeed, this model is particularly well-suited for our proposed studies because pediatric models of postnatal transmission have been developed and used to define the pathogenesis of infant HIV-1 infection and prevention strategies. However, NHP studies require extensive infrastructure and unique expertise. Thus, we the Program's Nonhuman Primate (NHP) Core (Core 1) will coordinate and implement all the NHP experiments proposed by the Program's two Projects: Project 1: “Origin and predictors of viral rebound in infants”, (PI: Dr. Ann Chahroudi, Emory University), and Project 2: “Impact of immune-based intervention on viral rebound in orally SHIV-infected infant monkeys” (PI: Dr. Genevieve Fouda, Duke University). The Core will support the Program through the following Specific Aims: Aim 1 - Organize, coordinate, and conduct studies of infant SHIV reservoir and rebound; Aim 2 - Administer and monitor antiretroviral treatment (ART) in orally SHIV-infected infant monkeys; and Aim 3 - Perform pharmacokinetic analysis of polyclonal antibody infusions in infant macaques to determine the dose and administration interval for treatment of infant SHIV infection. Led by Dr. Guido Silvestri, the NHP Core will ensure the following that are essential to the successful completion of these projects: 1) adherence to regulatory procedures relevant to research with NHPs; 2) coordination and implementation of all animal-related procedures, including housing, immunizations of infants, nursery-rearing of infants, antiretroviral treatments, and sample collections for weekly viral load measurement and monthly blood counts; 3) proper storage and/or shipment of samples to the Projects and Cores; and 4) maintenance of a database of clinical health parameters. Dr. Silvestri will ensure all NHP supported research efforts are tightly integrated and that evolving needs of the research projects will be met. As an integral component of this Program, the NHP Core will provide critical support to achieve the shared goals of understanding the source, kinetics, and impact of immune interventions on HIV-1 rebound in postnatally infected infants.