RhoE-mediated Sterile Inflammation Regulation in Acute Myocardial Infarction.
RhoE-mediated Sterile Inflammation Regulation in Acute Myocardial Infarction.
批准号:
10197204
负责人:
Jiang Chang
金额:
$37.13万
依托单位国家:
美国
项目类别:
财政年份:
2018
资助国家:
美国
项目状态:
已结题
起止时间:
2018-07-01 至 2024-06-30
关键词:
Acute myocardial infarctionAmericanAmerican Heart AssociationAngioplastyAnimal GeneticsAnimal ModelAnimalsAttenuatedBindingCardiacCardiovascular DiseasesCell Culture TechniquesClinicalComplexDataDown-RegulationExhibitsFoundationsFutureGap JunctionsGenesGeneticHeartHeart failureHumanIn VitroInfarctionInfiltrationInflammasomeInflammationInflammatoryInflammatory ResponseInterleukin-1 betaKnock-outLeadLinkMass Spectrum AnalysisMediatingModernizationMolecularMonomeric GTP-Binding ProteinsMusMutant Strains MiceMyocardial InfarctionNuclear ImportPatientsPharmacologyPhenotypePlayPost-Translational RegulationProcessPrognosisProtein AnalysisProteinsProteomicsRecoveryRecurrenceRegulationReportingRhoE proteinRoleSeriesSignal TransductionSterilityTNF geneTNFRSF5 geneTRIM MotifTestingTherapeuticTimeTissuesTransgenic MiceTranslationsUbiquitinationbasecohortcytokineexperimental studygain of functiongenetic manipulationhealingheart functionimprovedin vivoinjury recoveryinnovationinsightmacrophagemembermutantneutrophilnoveloverexpressionp65percutaneous coronary interventionpreventprotein complexprotein degradationprotein protein interactionreceptorrecruitrhosensorubiquitin-protein ligasewound healing
中文摘要
项目描述/摘要
根据 2016 年美国心脏协会报告,大约有 55 万美国人新患心脏病
心肌梗塞 (MI)(定义为首次住院 MI)和 200,000 例复发病例。大约,
每 42 秒就有一名美国人发生心肌梗死。虽然影响 MI 患者预后的因素有很多,
两个主要决定因素是初始梗塞范围和心肌梗死后恢复过程的效率。
临床上,现代经皮冠状动脉介入治疗(PCI),即血管成形术,可显着降低
梗塞的程度。然而,随后的伤口愈合过程是启动和驱动的
由动态无菌炎症引起的疾病是复杂且难以捉摸的。更好地了解无菌性炎症
急性心肌梗死期间的分子调控非常重要,为心肌梗死后各个阶段奠定基础
疗法。
在此提案中,我们开发了一种心脏特异性 RhoE(一种小 G 蛋白,也称为 Rnd3)缺陷-
易受 MI 影响的小鼠品系。 RhoE单倍体不足的小鼠表现出强烈的炎症反应
MI 后心脏功能受损。
我们提出了多种系统方法,包括蛋白质-蛋白质相互作用的体外分析、细胞
培养实验和体内遗传动物评估以及功能丧失和获得策略
研究 RhoE 表达水平与 MI 后炎症调节之间的关系。
RhoE介导的无菌性炎症调节的详细分子机制将得到阐明。
RhoE 首次与炎症调节联系起来。我们建议RhoE是一个新的“微调”
位于炎症反应连接点的因子,负责平衡无菌性炎症
急性心肌梗死后。
动物模型包括心脏特异性条件性 RhoE 缺陷小鼠以及心脏特异性 RhoE-
过度表达转基因小鼠。该提案的机制研究结果应具有临床意义
鉴于心力衰竭患者 RhoE 显着下调的事实。该发现将提供
为未来的药理学翻译奠定基础。
英文摘要
PROJECT DESCRIPTION/ABSTRACT
Based on the 2016 American Heart Association report, there are about 550,000 Americans newly suffering
from myocardial infarction (MI) (defined as first hospitalized MI) and 200,000 recurrent cases. Approximately,
one American will have an MI every 42 seconds. While many factors contribute to the prognosis of MI patients,
two major determinants are the initial infarction size and the efficiency of the post-MI recovery process.
Clinically, modern percutaneous coronary intervention (PCI), known as angioplasty, significantly decreases
the extent of the infarction. However, the subsequent wound healing processes, which are initiated and driven
by dynamic sterile inflammation, are complicated and elusive. A better understanding of sterile inflammation
molecular regulation during acute MI is highly significant, and lays the foundation for all stages of post-MI
therapeutics.
In this proposal, we have developed a cardiac-specific RhoE (a small G protein, also called Rnd3) deficient-
mouse line that is susceptible to MI. RhoE haploinsufficient mice exhibit an intense inflammatory response with
compromised cardiac function after MI.
We propose multiple systemic approaches including in vitro analysis of protein-protein interactions, cell
culture experiments, and in vivo genetic animal assessments with loss- and gain-of-function strategies to
investigate the relationship between the expression levels of RhoE and the regulation of post-MI inflammation.
The detailed molecular mechanism of RhoE-mediated sterile inflammation regulation will be elucidated.
For the first time, RhoE is linked to inflammation regulation. We suggest that RhoE is a new “fine-tuning”
factor situated at the nexus of the inflammatory response, and is responsible for balanced sterile inflammation
after acute MI.
The animal models include heart specific conditional RhoE-deficient mice as well as heart specific RhoE-
overexpression transgenic mice. The mechanistic findings from this proposal should result in clinical implications
given the fact of significant downregulations of RhoE in heart failure patients. The discovery will provide a
foundation for future pharmacological translation.
期刊论文(1)
专著(0)
科研奖励(0)
会议论文
DOI:
10.1038/s42003-020-01295-4
发表时间:
2020-10-15
期刊:
Communications biology
影响因子:
5.9
作者:
[Dai Y, Luo W, Yue X, Ma W, Wang J, Chang J]
通讯作者:
Chang J
Profiling communication networks of endogenous exosomes
-
批准号:10188126
-
项目类别:
-
资助金额:$18.94万
-
财政年份:2021
-
负责人:Jiang Chang
-
依托单位:
Profiling communication networks of endogenous exosomes
-
批准号:10394353
-
项目类别:
-
资助金额:$22.73万
-
财政年份:2021
-
负责人:Jiang Chang
-
依托单位:
Epigenetic signaling, pathological cardiac hypertrophy and Western diet
-
批准号:10132386
-
项目类别:
-
资助金额:$56.23万
-
财政年份:2020
-
负责人:Jiang Chang
-
依托单位:
Epigenetic signaling, pathological cardiac hypertrophy and Western diet
-
批准号:10374047
-
项目类别:
-
资助金额:$53.96万
-
财政年份:2020
-
负责人:Jiang Chang
-
依托单位:
Epigenetic signaling, pathological cardiac hypertrophy and Western diet
-
批准号:10593054
-
项目类别:
-
资助金额:$54.42万
-
财政年份:2020
-
负责人:Jiang Chang
-
依托单位:
Epigenomic signaling and heart failure.
-
批准号:10310475
-
项目类别:
-
资助金额:$48.58万
-
财政年份:2019
-
负责人:Jiang Chang
-
依托单位:
Epigenomic signaling and heart failure.
-
批准号:10528446
-
项目类别:
-
资助金额:$48.73万
-
财政年份:2019
-
负责人:Jiang Chang
-
依托单位:
Mechanistic Role of Rnd3 in Response to Cardiac Stress
-
批准号:8755080
-
项目类别:
-
资助金额:$38.64万
-
财政年份:2014
-
负责人:Jiang Chang
-
依托单位:
Mechanistic Role of Rnd3 in Response to Cardiac Stress
-
批准号:8890878
-
项目类别:
-
资助金额:$42.23万
-
财政年份:2014
-
负责人:Jiang Chang
-
依托单位:
Mechanistic Role of Rnd3 in Response to Cardiac Stress
-
批准号:9281046
-
项目类别:
-
资助金额:$42.87万
-
财政年份:2014
-
负责人:Jiang Chang
-
依托单位:
Mechanism of SRF-N-mediated Cardiac Suppression
-
批准号:8464206
-
项目类别:
-
资助金额:$9.48万
-
财政年份:2010
-
负责人:Jiang Chang
-
依托单位:
Mechanism of SRF-N-mediated Cardiac Suppression
-
批准号:8299035
-
项目类别:
-
资助金额:$9.48万
-
财政年份:2010
-
负责人:Jiang Chang
-
依托单位:
Mechanism of SRF-N-mediated Cardiac Suppression
-
批准号:8676899
-
项目类别:
-
资助金额:$9.48万
-
财政年份:2010
-
负责人:Jiang Chang
-
依托单位:
Mechanistic Study of Cleaved Serum Response Factor in Cardiac Hypertrophy
-
批准号:8061975
-
项目类别:
-
资助金额:$25.64万
-
财政年份:2010
-
负责人:Jiang Chang
-
依托单位:
Fibrogenic Role of ROCK delta1 and Mechanism in Cardiac Remodeling
-
批准号:8666794
-
项目类别:
-
资助金额:$35.53万
-
财政年份:2010
-
负责人:Jiang Chang
-
依托单位:
Fibrogenic Role of ROCK delta1 and Mechanism in Cardiac Remodeling
-
批准号:8067865
-
项目类别:
-
资助金额:$36.63万
-
财政年份:2010
-
负责人:Jiang Chang
-
依托单位:
Fibrogenic Role of ROCK delta1 and Mechanism in Cardiac Remodeling
-
批准号:8284368
-
项目类别:
-
资助金额:$36.26万
-
财政年份:2010
-
负责人:Jiang Chang
-
依托单位:
Mechanism of SRF-N-mediated Cardiac Suppression
-
批准号:7782900
-
项目类别:
-
资助金额:$9.22万
-
财政年份:2010
-
负责人:Jiang Chang
-
依托单位:
Fibrogenic Role of ROCK delta1 and Mechanism in Cardiac Remodeling
-
批准号:7865741
-
项目类别:
-
资助金额:$36.63万
-
财政年份:2010
-
负责人:Jiang Chang
-
依托单位:
Mechanism of SRF-N-mediated Cardiac Suppression
-
批准号:8098138
-
项目类别:
-
资助金额:$9.48万
-
财政年份:2010
-
负责人:Jiang Chang
-
依托单位:
海外基金