Microsurgery Core

项目总结/摘要 小鼠器官移植的显微外科模型在推进我们的研究方面发挥了关键作用 了解调节移植物排斥和耐受的免疫途径。等 模型是为移植患者开发治疗方法的重要工具。作为实质性的差异 存在于对各种移植物的免疫反应之间，在一个器官中获得的见解不能被 翻译到其他机构。我们的实验室已经开发出临床相关的肺部模型， 移植和再移植的小鼠以及新的方法，以图像免疫 小鼠肺移植物中真实的时间的细胞相互作用。我们和其他人用这些 研究临床相关的肺特异性同种免疫应答的方法。专用 显微外科核心是在2015年该计划项目启动时建立的。的 显微外科核心已被证明有助于促进实验的执行。在aim中 1我们将继续标准化的质量显微外科和成像程序的所有三个 提案中的项目。在目标2中，我们将继续标准化移植物功能评估， 三个项目的组织采集在目标3中，我们将维持一个系统， 获得显微外科移植，并协调项目1的活体成像程序 和3.

PROJECT SUMMARY/ABSTRACT Microsurgical models of murine organ transplantation have played a critical role in advancing our understanding of immunological pathways that regulate graft rejection and tolerance. Such models are important tools to develop therapies for transplant patients. As substantial differences exist between immune responses to various grafts, insights gained in one organ cannot be translated to other organs. Our laboratories have developed clinically relevant models of lung transplantation and re-transplantation in the mouse as well as novel approaches to image immune cell interactions in the murine pulmonary graft in real time. We and others have used these approaches to investigate clinically relevant lung-specific alloimmune responses. A dedicated microsurgery core was established at the initiation of this program project in 2015. The microsurgery core has proven instrumental in facilitating the execution of the experiments. In aim 1 we will continue to standardize the quality of microsurgical and imaging procedures for all three projects in the proposal. In aim 2 we will continue to standardize assessment of graft function and tissue harvest for all three projects. In aim 3 we will maintain a system that provides unfettered access to microsurgical transplants and coordinates intravital imaging procedures for Projects 1 and 3.