Cell and Tissue Imaging Core

项目总结：细胞和组织成像核心A 先进的细胞显微镜是生物学研究的有力工具， 对人类疾病的发病机理和最终治疗的研究。成像技术的发展 在过去的十年中迅速导致分辨率，灵敏度和速度的提高， 为研究跨越多个数量级的过程创造了全新的机会， 在活细胞和生物体中实时进行。细胞和组织成像核心A由共享的 尖端的多尺度显微镜设施，这是必不可少的3个项目的研究目标，在这个 P01更新申请，标题为“α 1-AT中肝纤维化和过度增殖的新疗法” 缺乏症（ATD）。该设施位于华盛顿大学细胞成像中心 （WUCCI）（http：wucci.wustl.edu），以利用最近在华盛顿的重大机构投资 大学医学院，以及提供一个综合的方法来调查结构和 ATD特异性细胞和组织的动态行为。本P01更新计划中的所有项目都将 使用WUCCI提供的各种细胞显微镜方法。现有方法 项目PI包括光学显微镜模式，如荧光显微镜，共聚焦和双- 光子显微镜，活细胞显微镜（共聚焦和全内反射- TIRF），超 分辨率显微镜（PALM / STORM和SIM）和载玻片扫描显微镜（亮场和 荧光）。其他先进的荧光模式，如FRET，FRAP，光谱和比率测量 图像也是可用的。一个完整的阵列超微结构的方法也可用于项目 investigators.这些包括透射和扫描电子显微镜（TEM和SEM）， 负染法、传统薄切片电镜、免疫电镜、连续块面聚焦离子束扫描电镜（FIB-100）， 扫描电镜（SEM）、X射线显微镜、单颗粒冷冻电镜和冷冻电子断层扫描（ET）。而且 该设施提供了一个广泛的图像分析和定量软件，以及技术 协助设施工作人员正确使用。由于设施、设备和工作人员的专业知识 位于一个连续的位置，可以实现成像模态之间的无缝集成。 经过培训的用户可以通过ID卡24/7全天候访问该设施，他们还可以预订仪器时间或提交样品- 通过我们基于Web的在线电子管理解决方案Agilent CrossLab随时处理请求 （原iLab解决方案）。

PROJECT SUMMARY: Cell and Tissue Imaging Core A Advanced cellular microscopy is a powerful tool for biological research and has an important role to play in the study of the pathogenesis and eventual treatment of human diseases. Imaging technology has evolved rapidly over the last decade leading to improvements in resolution, sensitivity and speed which have created fundamentally new opportunities for studying processes across many orders of magnitude and in real-time in living cells and organisms. The Cell and Tissue Imaging Core A is comprised of a shared cutting-edge multi-scale microscopy facility that is essential to the research goals of the 3 projects in this P01 renewal application entitled “New Therapies for liver fibrosis and hyperproliferation in alpha1-AT deficiency (ATD)”. The facility is housed within the Washington University Center for Cellular Imaging (WUCCI) (http://wucci.wustl.edu), to leverage recent significant institutional investments at Washington University School of Medicine as well as to provide an integrated approach to investigating the structure and dynamic behavior of ATD specific cells and tissues. All projects within this P01 renewal plan to make heavy use of the diverse array of cellular microscopy approaches available in the WUCCI. Methodologies available to project PIs include optical microscopy modalities such as fluorescence microscopy, confocal and two- photon microscopy, live-cell microscopy (both confocal and Total Internal Reflection - TIRF), super- resolution microscopy (both PALM / STORM and SIM) and slide-scanning microscopy (both bright-field and fluorescence). Additional advanced fluorescence modalities such as FRET, FRAP, spectral and ratiometric imaging are also available. A complete array of ultrastructural approaches is also available to project investigators. These comprise both transmission and scanning electron microscopy (TEM and SEM), negative staining, traditional thin section EM, immuno-EM, serial block face Focused Ion Beam-SEM (FIB- SEM), X-ray microscopy, single particle cryo-EM and cryo-Electron Tomography (ET). Furthermore, the facility provides access to a wide range of image analysis and quantitation software as well as technical assistance in their appropriate use from facility staff. Since the facility, the equipment and the staff expertise reside in one contiguous location, seamless integration between the imaging modalities can be realized. The facility is accessible ID card 24/7 by trained users who can also book instrument time or submit sample- processing requests anytime through our on-line web-based e-management solution, Agilent CrossLab (formerly iLab solutions).