Cellular Imaging Core

细胞成像核心

• 批准号: 10019351
• 负责人: James Alexander Fitzpatrick
• 金额: $ 22.14万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10019351
• 关键词: 3-Dimensional; 4D Imaging; Animal Model; Animals; Area; Basic Science; Biological Assay; Biological Models; Cell physiology; Cells; Characteristics; Consultations; Consumption; Contrast Media; Custom; Data Analyses; Data Set; Development; Disease; Educational workshop; Electron Microscopy; Electrons; Ensure; Equipment; Experimental Designs; Feedback; Funding; Generations; Goals; Human Resources; Image; Image Analysis; Imaging Techniques; Imaging technology; Immunofluorescence Microscopy; Individual; Informatics; Investments; Ions; Joints; Knowledge; Laboratories; Laboratory Research; Light; Logistics; Methodology; Microscope; Microscopic; Microscopy; Morphology; Optics; Organ; Pathogenesis; Play; Preparation; Process; Research; Research Personnel; Resolution; Resources; Rheumatism; Roentgen Rays; Role; Sampling; Services; Specialist; Specimen; Speed; Structure; System; Technical Expertise; Techniques; Technology; Time; Tissue imaging; Tissues; Training; Translating; Translational Research; Universities; Visualization; Washington; Work; X ray microscopy; base; biological research; cell behavior; cellular imaging; cost; data acquisition; imaging modality; imaging study; improved; innovation; instrumentation; light microscopy; member; microscopic imaging; molecular imaging; novel; operation; programs; quantitative imaging; research study; response; single molecule; soft tissue; tool; translational study; two-dimensional; two-photon

PROJECT SUMMARY: Cellular Imaging Core Advanced cellular microscopy is a powerful tool for biological research and has an important role to play in the study of disease pathogenesis, which may translate eventually to novel treatment approaches for rheumatic diseases. Imaging technology has evolved rapidly over the last decade leading to improvements in resolution, sensitivity and speed, which have created fundamentally new opportunities for studying processes across many orders of magnitude and in real-time in living cells and animals. At the same time, the costs of increasingly sophisticated equipment are substantial and the expertise to efficiently use, maintain, and develop this equipment is not common in most labs. There is, therefore, a significant gap between the availability of these powerful tools and the ability of individual investigators to access and use them efficiently. The Cellular Imaging Core was created in response to feedback from Rheumatic Diseases Research Resource-based Center members to leverage the significant institutional investment in the newly created Washington University Center for Cellular Imaging (WUCCI) and provide an integrated approach to investigate the structure and dynamic behavior of cells and tissues rheumatic disease-related model systems. The overall objective of the Core is to provide access to and technical support in using advanced cellular microscopy tools to accelerate the pace, expand the scope, and improve efficiency of rheumatic diseases research. The Core services meet the unique requirements of numerous investigators over a wide range of basic and translational research, attracting new investigators into rheumatic diseases research areas. Importantly the users benefit from the in-depth technical expertise of the Core Director and technical staff in the time spent in consultation for experimental design and interpretation of data. During the last fiscal year (July 1 2016 – June 30 2017) of WUCCI operations, the Core served 184 research laboratories, 11 of these were RDRRC members whose usage represented ~10% of the overall core consumption. It is anticipated that requests for Cellular Imaging Core services will continue to grow given the overwhelming need for advanced microscopic imaging and image analysis approaches in the study of rheumatic diseases. The Cellular Imaging Core will provide essential services that will enable and accelerate the research of RDRRC investigators throughout the next funding cycle.

项目总结：细胞成像核心 先进的细胞显微镜是生物学研究的有力工具， 疾病发病机制的研究，这可能最终转化为新的治疗方法， 风湿性疾病成像技术在过去十年中发展迅速， 在分辨率、灵敏度和速度方面，这为研究创造了全新的机会， 在活细胞和动物中实时进行许多数量级的过程。与此同时， 日益复杂的设备的成本是巨大的，并且有效使用的专业知识， 维护和开发这种设备在大多数实验室并不常见。因此， 这些强大的工具的可用性和个人调查人员访问和使用 他们效率。细胞成像核心是根据风湿性疾病的反馈创建的 研究资源为基础的中心成员，以利用重大机构投资，在新的 创建了华盛顿大学细胞成像中心（WUCCI），并提供了一种综合方法， 研究风湿病相关模型细胞和组织的结构和动力学行为 系统.该核心的总体目标是提供使用先进技术的机会和技术支持， 细胞显微镜工具，以加快步伐，扩大范围，提高效率的风湿性关节炎 疾病研究。核心服务满足了广泛领域内众多调查人员的独特要求 一系列基础和转化研究，吸引新的研究人员进入风湿性疾病的研究 地区重要的是，用户受益于核心主任和技术人员的深入技术专长， 工作人员在咨询实验设计和解释数据所花费的时间。在过去 WUCCI运营的一个财政年度（2016年7月1日至2017年6月30日），核心服务于184个研究实验室， 其中11个是RDRRC成员，其使用量占核心消费总量的约10%。是 预计对细胞成像核心服务的需求将继续增长， 在风湿性疾病的研究中需要先进的显微成像和图像分析方法。 细胞成像核心将提供必要的服务，这将使和加速研究 在整个下一个供资周期，恢复民主和重返社会委员会的调查员将继续工作。