Cell and Tissue Imaging Core

项目摘要：细胞和组织成像核心A 先进的细胞显微镜是生物学研究的有力工具，在 对人类疾病的发病机制和最终治疗方法的研究。成像技术已经发展到 在过去的十年中迅速地导致了分辨率、灵敏度和速度的改进，这些都 为研究多个数量级的过程创造了全新的机会 在活细胞和生物体中的实时。细胞和组织成像核心A由共享的 尖端的多尺度显微镜设备，对本项目的3个项目的研究目标至关重要 P01续展申请，题为《Alpha1-AT中肝纤维化和过度增殖的新疗法》 缺乏(ATD)“。该设施位于华盛顿大学细胞成像中心内 (WUCCI)(http://wucci.wustl.edu)，将利用华盛顿最近的重大机构投资 以及提供一种综合的方法来研究结构和 ATD特定细胞和组织的动态行为。本次P01更新计划内的所有项目都将重磅 使用WUCCI提供的各种细胞显微镜方法。可用的方法 投射PI包括光学显微镜形式，如荧光显微镜、共聚焦和两种- 光子显微镜，活细胞显微镜(共聚焦和全内反射-TIRF)，超 分辨率显微镜(Palm/Storm和SIM)和载玻片扫描显微镜(明视场和 荧光)。其他高级荧光模式，如FRET、FRAP、光谱和比率测量法 此外，还提供成像功能。一系列完整的超微结构方法也可用于项目 调查人员。其中包括透射和扫描电子显微镜(透射电子显微镜和扫描电子显微镜)， 阴性染色，传统薄片EM，免疫-EM，连续块脸聚焦离子束扫描电子显微镜(FIB-SEM) 扫描电子显微镜、X射线显微镜、单颗粒低温电子显微镜和低温电子断层扫描(ET)。此外， 设施提供广泛的图像分析和量化软件以及技术 从设施工作人员那里获得适当使用的帮助。由于设施、设备和工作人员的专业知识 驻留在一个连续的位置，可以实现成像模式之间的无缝集成。 训练有素的用户可以全天候使用身份证，他们还可以预约仪器时间或提交样品- 通过我们基于Web的在线电子管理解决方案Agilent CrossLab随时处理请求 (以前的iLab解决方案)。

PROJECT SUMMARY: Cell and Tissue Imaging Core A Advanced cellular microscopy is a powerful tool for biological research and has an important role to play in the study of the pathogenesis and eventual treatment of human diseases. Imaging technology has evolved rapidly over the last decade leading to improvements in resolution, sensitivity and speed which have created fundamentally new opportunities for studying processes across many orders of magnitude and in real-time in living cells and organisms. The Cell and Tissue Imaging Core A is comprised of a shared cutting-edge multi-scale microscopy facility that is essential to the research goals of the 3 projects in this P01 renewal application entitled “New Therapies for liver fibrosis and hyperproliferation in alpha1-AT deficiency (ATD)”. The facility is housed within the Washington University Center for Cellular Imaging (WUCCI) (http://wucci.wustl.edu), to leverage recent significant institutional investments at Washington University School of Medicine as well as to provide an integrated approach to investigating the structure and dynamic behavior of ATD specific cells and tissues. All projects within this P01 renewal plan to make heavy use of the diverse array of cellular microscopy approaches available in the WUCCI. Methodologies available to project PIs include optical microscopy modalities such as fluorescence microscopy, confocal and two- photon microscopy, live-cell microscopy (both confocal and Total Internal Reflection - TIRF), super- resolution microscopy (both PALM / STORM and SIM) and slide-scanning microscopy (both bright-field and fluorescence). Additional advanced fluorescence modalities such as FRET, FRAP, spectral and ratiometric imaging are also available. A complete array of ultrastructural approaches is also available to project investigators. These comprise both transmission and scanning electron microscopy (TEM and SEM), negative staining, traditional thin section EM, immuno-EM, serial block face Focused Ion Beam-SEM (FIB- SEM), X-ray microscopy, single particle cryo-EM and cryo-Electron Tomography (ET). Furthermore, the facility provides access to a wide range of image analysis and quantitation software as well as technical assistance in their appropriate use from facility staff. Since the facility, the equipment and the staff expertise reside in one contiguous location, seamless integration between the imaging modalities can be realized. The facility is accessible ID card 24/7 by trained users who can also book instrument time or submit sample- processing requests anytime through our on-line web-based e-management solution, Agilent CrossLab (formerly iLab solutions).