Macromolecular Crystallography Lab

项目总结 (高分子结晶学实验室) 用X-射线衍射仪测定蛋白质、核酸等大分子的三维结构 射线结晶学为了解分子或分子复合体的功能提供了独特的见解。OU- 诺曼高分子结晶学实验室核心(MCL)提供专业知识和最先进的 支持俄克拉荷马州科布雷结构生物学(OCSB)附属研究小组和 参与结构生物学研究的其他研究小组，特别是在蛋白质结晶学方面， 俄克拉荷马州。MCL通过利用机器人仪器进行筛选来实现这一点 结晶条件、高通量微聚焦X射线发生器和最先进的混合器的使用 用于数据收集的像素X射线探测器以及最新的计算资源维护，以 加工并确定感兴趣的大分子的结构。MCL主任，伦纳德博士 托马斯是一名全职博士结晶学家。该设施还聘请了一名研究生助理来协助 结晶条件的筛选和用于收集X射线数据的样品的制备。MCL 为对大分子结构感兴趣的学生、博士后研究员和教职员工提供培训 决心。在OCSB第一阶段资助期间，MCL用户基础得到了极大的扩大， 设施将继续通过提供专业知识、最先进的设施和培训来扩大其用户基础 有组织及严重罪行条例草案第二阶段。

PROJECT SUMMARY (Macromolecular Crystallography Laboratory) The three-dimensional structure determination of macromolecules such as proteins and nucleic acids using X- ray crystallography provides unique insights into the function of the molecule or molecular complex. The OU- Norman Macromolecular Crystallography Laboratory Core (MCL) provides expertise and state-of-the-art instrumentation to support the Oklahoma COBRE in Structure Biology (OCSB) affiliated research groups and other research groups involved in structural biology research, particularly in protein crystallography, across the State of Oklahoma. The MCL accomplishes this by utilizing robotic instrumentation for the screening of crystallization conditions, the utilization of a high flux micro focus X-ray generator and a state-of-the-art hybrid pixel X-ray detector for data collection along with up to date maintenance of computational resources to process and determine the structure of the macromolecules of interest. The MCL director, Dr. Leonard Thomas, is a full time Ph.D. crystallographer. The facility also employs a Graduate Assistant to aid in the screening of crystallization conditions and the preparation of samples for collection of X-ray data. The MCL provides training to students, postdoctoral fellows and faculty who are interested in macromolecular structure determination. During the OCSB Phase I funding the MCL user base has been greatly expanded and the facility will continue to expand its user base by providing expertise, state-of-the-art facilities and training during the OCSB Phase II.