OU Macromolecular X-ray Facility

OU-Norman高分子结晶学实验室(MCL)作为科布雷核心的发展 设施将使其能够继续提供大分子样品结晶方面的专业知识和 为俄克拉荷马大学的研究小组和整个 俄克拉荷马州。该设施将为初级调查员(JLS)、早期职业调查员和其他 研究小组参与了结构生物学，这是一种推进其结构研究的最先进的设施。 MCL可以指导研究小组从结晶板的初始设置到解决方案，并 对最终的三维结构进行解释。MCL目前提供的能力是高 通过利用机器人仪器实现产量结晶。机器人技术的应用 结晶允许在确定大分子结晶条件时有效地使用样品。 晶体可以通过MCL目前的衍射仪设置进行筛选，但我们正在提议升级我们的X光 发生器能够为我们的初级研究人员提供尽可能最好的X射线结晶学设备 以及该州的其他结构生物学调查人员。强度的增加将使所有调查人员受益 通过提供筛选和收集较小的和一般的X射线衍射数据的能力 较弱的绕射晶体。除了在更高强度的发电机上使用我们的电流探测器外，我们 还建议将其耦合到ccd探测器，以提供现代高效的衍射系统，以允许 JL‘s和其他研究人员在全州范围内进行结构生物学研究。

The development ofthe OU-Norman Macromolecular Crystallography Laboratory (MCL) as a COBRE Core facility will allow it to continue to provide expertise in both the crystallization of macromolecular samples and the collection of X-ray diffraction data for research groups at the University of Oklahoma and throughout the State of Oklahoma. This facility will provide the Junior Investigators (Jls), early career Investigators and other research groups involved in structural biology a state-of-the-art facility to advance their structural studies. The MCL can guide the research groups from the initial setup of crystallization plates to the solution and interpretation of the final three-dimensional structures. The MCL currently provides the capability of high throughput crystallization by the utilization of robotics Instrumentation. Utilization of the robotics for crystallization allows for efficient use of sample in determining macromolecular crystallization conditions. Crystals can be screened by MCL's current diffractometer setup but we are proposing an upgrade of our Xray generator to be able to provide the best possible X-ray crystallography facility for our Junior Investigators and other structural biology Investigators In the State. The increase In Intensity will benefit all investigators by providing the capability of screening and collection of X-ray diffraction data on smaller and generally weaker diffracting crystals. In addition to utilizing our current detector on the higher intensity generator we are also proposing to couple it to a CCD detector to provide a modern efficient diffraction system to allow the Jl's and other investigators statewide to conduct their research In structural biology.