OU Macromolecular X-ray Facility

OU高分子X射线设备

• 批准号: 8666001
• 负责人: Leonard M Thomas
• 金额: $ 45.64万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8666001
• 关键词: Centers of Research Excellence; Collection; Core Facility; Crystallization; Crystallography; Data; Development; Laboratories; Oklahoma; Research; Research Personnel; Robotics; Roentgen Rays; Sampling; Solutions; System; Universities; X ray diffraction analysis; X-Ray Crystallography; X-Ray Diffraction; career; detector; instrumentation; sample collection; screening; structural biology; three dimensional structure

The development ofthe OU-Norman Macromolecular Crystallography Laboratory (MCL) as a COBRE Core facility will allow it to continue to provide expertise in both the crystallization of macromolecular samples and the collection of X-ray diffraction data for research groups at the University of Oklahoma and throughout the State of Oklahoma. This facility will provide the Junior Investigators (Jls), early career Investigators and other research groups involved in structural biology a state-of-the-art facility to advance their structural studies. The MCL can guide the research groups from the initial setup of crystallization plates to the solution and interpretation of the final three-dimensional structures. The MCL currently provides the capability of high throughput crystallization by the utilization of robotics Instrumentation. Utilization of the robotics for crystallization allows for efficient use of sample in determining macromolecular crystallization conditions. Crystals can be screened by MCL's current diffractometer setup but we are proposing an upgrade of our Xray generator to be able to provide the best possible X-ray crystallography facility for our Junior Investigators and other structural biology Investigators In the State. The increase In Intensity will benefit all investigators by providing the capability of screening and collection of X-ray diffraction data on smaller and generally weaker diffracting crystals. In addition to utilizing our current detector on the higher intensity generator we are also proposing to couple it to a CCD detector to provide a modern efficient diffraction system to allow the Jl's and other investigators statewide to conduct their research In structural biology.

作为COBRE核心的OU-Norman高分子晶体学实验室（MCL）的发展 该设施将使其能够继续提供高分子样品结晶方面的专业知识， 为俄克拉荷马州大学和整个 俄克拉荷马州。该机构将为初级研究者（JLS）、早期职业研究者和其他 结构生物学研究小组拥有先进的设施，以推进其结构研究。 MCL可以指导研究小组从初始设置结晶板到解决方案， 最终三维结构的解释。MCL目前提供高性能 通过利用机器人仪器进行生产量结晶。利用机器人技术 结晶允许在确定大分子结晶条件中有效地使用样品。 晶体可以通过MCL目前的衍射仪设置进行筛选，但我们建议升级我们的X射线衍射仪。 发生器能够为我们的初级研究人员提供最好的X射线晶体学设备 和其他的结构生物学研究者强度的增加将使所有研究人员受益 通过提供筛选和收集更小且通常 较弱的衍射晶体。除了在更高强度的发生器上使用我们的电流检测器外， 还建议将其耦合到CCD检测器，以提供现代有效的衍射系统， Jl和其他全州的研究人员进行他们的结构生物学研究。