Rapid‐Response Macromolecular Crystallography

项目摘要/摘要 – 技术运营核心 1 (TOC1) 拟议的 ALS-ENABLE 资源的目标是提供一个集成的、最先进的结构 为全国 NIH 研究人员提供的生物学资源。 TOC1 特别汇集了八个 ALS 将大分子晶体学 (MX) 同步加速器光束线整合为一个国家资源，创建统一的 该计划将单个光束线的强度扩展到整个资源，同时提供单一的 接口，以满足用户社区对波束时间、数据收集和数据处理的需求。肌萎缩侧索硬化症 结构生物学光束线是最先进的“成熟”资源，共同为数百名用户提供服务 每年，由专门的光束线科学家与 NIH 资助的 PI 进行长期合作 国家。 TOC1 中的三个目标将八个资源开发的当前自动化技术联系起来 将光束线整合为一个完整的自动化管道，将远程访问晶体学扩展到所有资源 光束线，并将当前的协作晶体学工作形式化并统一到一个程序中。 ALS- ENABLE 资源首次将 ALS 上的四个独立 MX 组聚集在一起，其中的八个 终端站和协作晶体学计划整合为一项国家资源。这种整合 ALS 的结构生物学同步加速器光束线可实现显着的协同效应和效率。它建立在 结构生物学光束线目前的成功，使结构生物学解决方案能够在最 具有挑战性和相关的生物学问题，从而服务于国家生物医学研究的需要 社区。

Project Summary/Abstract – Technology Operations Core 1 (TOC1) The goal of the proposed ALS-ENABLE resource is to provide an integrated and state-of-the-art structural biology resource for NIH investigators across the country. TOC1 in particular brings together the eight ALS macromolecular crystallography (MX) synchrotron beamlines into one national resource, creating a unified program which extends the individual beamline strengths to the entire resource, while providing a single interface to meet the user community needs for beamtime, data collection and data processing. The ALS structural biology beamlines are state-of-the-art "mature" resources, collectively serving hundreds of users annually, with dedicated beamline scientists with strong long-term collaborations with NIH-funded PIs across the country. The three aims in TOC1 link the current automation technologies developed at the eight resource beamlines into one complete automation pipeline, extend remote access crystallography to all the resource beamlines, and formalize and unify the current collaborative crystallography efforts into one program. The ALS- ENABLE resource brings together for the first time the four independent MX groups at the ALS, their eight endstations, and the Collaborative Crystallography program into one national resource. This integration of structural biology synchrotron beamlines at the ALS allows significant synergies and efficiencies. It builds on the current success of the structural biology beamlines, enabling structural biology solutions on the most challenging and relevant biological problems, thus serving the needs of the national biomedical research community.