Rapid‐Response Macromolecular Crystallography

项目摘要/摘要-技术运营核心1(TOC1) 拟议的支持ALS的资源的目标是提供一个集成的、最先进的结构 为全国各地的NIH调查人员提供生物资源。TOC1尤其汇集了8个肌萎缩侧索硬化 大分子结晶学(MX)将同步加速器光束线整合为一个国家资源，创造了统一的 该计划将单个光束线的优势扩展到整个资源，同时提供单一的 接口，以满足用户社区对波束时间、数据采集和数据处理的需求。肌萎缩侧索硬化 结构生物学光束线是最先进的“成熟”资源，共同服务于数百名用户 每年，与专门的光束线科学家与美国国立卫生研究院资助的PI进行强有力的长期合作 这个国家。TOC1中的三个目标将八个资源开发的当前自动化技术联系在一起 将光束线整合到一个完整的自动化管道中，将远程访问晶体结构扩展到所有资源 光束线，并将当前的合作结晶学工作正式和统一到一个计划中。肌萎缩侧索硬化- Enable RESOURCE首次将ALS的四个独立的MX小组聚集在一起，他们的八个小组 终端站和协作结晶学计划合并为一个国家资源。这一整合 ALS的结构生物学同步加速器光束线实现了显著的协同效应和效率。它建立在 目前结构生物学光束线的成功，使结构生物学解决方案能够最大限度地 具有挑战性和相关性的生物学问题，从而服务于国家生物医学研究的需要 社区。

Project Summary/Abstract – Technology Operations Core 1 (TOC1) The goal of the proposed ALS-ENABLE resource is to provide an integrated and state-of-the-art structural biology resource for NIH investigators across the country. TOC1 in particular brings together the eight ALS macromolecular crystallography (MX) synchrotron beamlines into one national resource, creating a unified program which extends the individual beamline strengths to the entire resource, while providing a single interface to meet the user community needs for beamtime, data collection and data processing. The ALS structural biology beamlines are state-of-the-art "mature" resources, collectively serving hundreds of users annually, with dedicated beamline scientists with strong long-term collaborations with NIH-funded PIs across the country. The three aims in TOC1 link the current automation technologies developed at the eight resource beamlines into one complete automation pipeline, extend remote access crystallography to all the resource beamlines, and formalize and unify the current collaborative crystallography efforts into one program. The ALS- ENABLE resource brings together for the first time the four independent MX groups at the ALS, their eight endstations, and the Collaborative Crystallography program into one national resource. This integration of structural biology synchrotron beamlines at the ALS allows significant synergies and efficiencies. It builds on the current success of the structural biology beamlines, enabling structural biology solutions on the most challenging and relevant biological problems, thus serving the needs of the national biomedical research community.