High throughput X-ray footprinting mass spectrometry (XFMS)
高通量 X 射线足迹质谱 (XFMS)
基本信息
- 批准号:10708045
- 负责人:
- 金额:$ 14.25万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-09-01 至 2027-08-31
- 项目状态:未结题
- 来源:
- 关键词:AutomationAwardBindingBiologicalBuffersChemistryCollectionCommunitiesComplex MixturesComputer softwareCrystallographyDataData AnalysesData CollectionDatabasesDeuteriumDevelopmentDiameterDigestionDoseExclusionExposure toFingerprintFluorescenceFluorescence SpectroscopyGrantHourHybridsHydrogenHydroxyl RadicalIn SituIndividualInvestigationKineticsLabelLaboratoriesLearningLiquid ChromatographyLiquid substanceMapsMass Spectrum AnalysisMethodsMolecular ConformationMolecular Sieve ChromatographyNational Institute of General Medical SciencesNucleic AcidsPositioning AttributeProcessProteinsProtocols documentationPumpRaman Spectrum AnalysisReportingResourcesRoentgen RaysSamplingShotgunsSignal TransductionSiteSolventsSpectrum AnalysisSpeedStructureSyringesSystemTechnologyTimeTubeX ray spectroscopybasebeamlinebiological systemschromophoredata accessexperimental studyflexibilityinstrumentinstrumentationliquid chromatography mass spectrometrymacromolecular assemblymacromoleculeoperationprotein complexprotein structuresample collectionspectroscopic datastructural biologysuccesstime usetoolweb site
项目摘要
Technology Operations Core 4: Abstract
Technology Operations Core 4 (TOC4) integrates the method of X-ray footprinting mass spectrometry (XFMS)
into the ALS-ENABLE resource, providing resource users with a solution state structural biology method that is
highly complementary to macromolecular crystallography (MX, TOCs1&3) and small angle X-ray scattering
(SAXS, TOC2). XFMS is an in situ hydroxyl radical (•OH) labeling method based on well-established solvent
accessibility protocols similar to Fenton chemistry and hydrogen deuterium exchange, sharing the same liquid
chromatography-mass spectrometry (LCMS) data collection platform. The method provides residue-level
structural information on proteins and/or nucleic acid in the solution state, and can be used for time-resolved
studies and on complex mixtures of proteins in solution. Because of its versatility, the method has been especially
valuable in obtaining structural and kinetics information that is highly complementary to the information obtained
using the other more well-known structural biology methods such as MX an SAXS. The XFMS method has a
vibrant, growing user base, has advanced substantially over the previous decade in automation and throughput,
and is now close to the same level of routine use as MX and SAXS. In the last four years in particular, the
instrumentation developed as part of a current NIGMS R01 award has significantly advanced the throughout and
accessibility of the method for the larger biomedical community, including the development of an automated
liquid sample delivery system, a remote-accessible beamline control interface, and automated LCMS data
analysis. Through TOC4, we will make available our automated, high throughput, and hybrid spectroscopy-
XFMS platform to ALS-ENABLE users through remote access, onsite and mail-in options. We will integrate
information about XFMS and access to databases through the ALS-ENABLE website (als-enable.lbl.gov) so that
users will have a single front-facing portal through which to learn about and access data from all the structural
biology beamlines at the ALS. The integration of these technologies within the ALS-ENABLE resource will
extend the structural biology capabilities at the ALS such that even more challenging biological systems can be
studied, thus providing the national biomedical community with a powerful and increasingly versatile new tool for
investigation of macromolecular assemblies.
技术运营核心4:摘要
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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CORIE Y RALSTON其他文献
CORIE Y RALSTON的其他文献
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{{ truncateString('CORIE Y RALSTON', 18)}}的其他基金
New assay method for pinpointing structural features in amyloid oligomer formation
精确定位淀粉样蛋白寡聚体形成结构特征的新测定方法
- 批准号:
10214240 - 财政年份:2021
- 资助金额:
$ 14.25万 - 项目类别:
Development of high-dose time-resolved X-ray footprinting technologies to enable detailed structural and kinetics information to be obtained for challenging biological problems
开发高剂量时间分辨 X 射线足迹技术,以获得具有挑战性的生物问题的详细结构和动力学信息
- 批准号:
10446793 - 财政年份:2018
- 资助金额:
$ 14.25万 - 项目类别:
Development of high-dose time-resolved X-ray footprinting technologies to enable detailed structural and kinetics information to be obtained for challenging biological problems
开发高剂量时间分辨 X 射线足迹技术,以获得具有挑战性的生物问题的详细结构和动力学信息
- 批准号:
10630950 - 财政年份:2018
- 资助金额:
$ 14.25万 - 项目类别:
High throughput X-ray footprinting mass spectrometry (XFMS)
高通量 X 射线足迹质谱 (XFMS)
- 批准号:
10506288 - 财政年份:2017
- 资助金额:
$ 14.25万 - 项目类别:
STRUCTURE OF TWO NIFE CLUSTERS IN CODH FROM CLOSTRIDIUM THERMOACETICUM
热乙酸梭菌 CODH 中两个 NIFE 簇的结构
- 批准号:
6658656 - 财政年份:2002
- 资助金额:
$ 14.25万 - 项目类别:
STRUCTURE OF TWO NIFE CLUSTERS IN CODH FROM CLOSTRIDIUM THERMOACETICUM
热乙酸梭菌 CODH 中两个 NIFE 簇的结构
- 批准号:
6586689 - 财政年份:2002
- 资助金额:
$ 14.25万 - 项目类别:
STRUCTURE OF TWO NIFE CLUSTERS IN CODH FROM CLOSTRIDIUM THERMOACETICUM
热乙酸梭菌 CODH 中两个 NIFE 簇的结构
- 批准号:
6437607 - 财政年份:2001
- 资助金额:
$ 14.25万 - 项目类别:
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