Storage and replay of information during SPW-Rs

在这个项目中，我们探索了在SPW-R期间如何重放信息的问题 表示该信息在学习期间可以如何以及在哪些子网络中被编码， 以及导致海马区信息存储的分子和网络机制 突触。项目3.1寻求开发一种使用新的记忆来人工诱导记忆的方法 光遗传学方法，允许小群神经元在清醒的行为动物中被兴奋。 衡量成功与否的标准将是在SPW-R期间是否重播人工记忆。的价值 这样的系统将为研究记忆的生物物理学提供一个受控系统 编码。项目3.2试图删除行为编码的记忆。最近的研究表明 记忆是通过丰富的突触蛋白CaMKII以突触方式储存的。这项工作是基于 显性负性CaMKII消除条件性位置回避的能力。我们将测试一下 CA3或DG中的显性负性CaMKII可消除海马区系统重放A 在SPW-R期间的记忆。项目3.3探讨了空间信息如何 在内存中表示。一种观点认为，空间记忆被表示为一条连续的路径。我们 将检验另一种假设，即记忆可以通过奖励网站选择性地代表。 项目3.4利用了识别细胞的长期光学记录方面的最新进展。 这将允许我们对存储序列的长期稳定性进行第一次观察 在SPW-R期间重放的内容，以及纵向标识网络元素 与这一长期重播相关联，从而提供了对 整合。

In this project we explore question of how the information replayed during the SPW-R is represented, how and in which sub-networks this information may get encoded during learning, and the molecular and network mechanisms that lead to storage of information at hippocampal synapses. Project 3.1 seeks to develop a method for artificially inducing a memory using new optogenetic methods that allow small groups of neurons to be excited in awake behaving animals. A measure of success will be if the artificial memory is replayed during a SPW-R. The value of such a system will be that it will provide a controlled system for studying the biophysics of memory encoding. Project 3.2 seeks to erase a behaviorally encoded memory. Recent work suggests that memory is stored synaptically by the abundant synaptic protein, CaMKII. This work was based on the ability of dominant negative CaMKII to erase conditioned place avoidance. We will test whether dominant negative CaMKII in CA3 or DG can erase the ability of hippocampal system to replay a memory during the SPW-R. Project 3.3 explores the question of how spatial information is represented in memory. One view is that spatial memory is represented as a continuous path. We will test the alternative hypothesis that memory can be represented selectively by reward sites. Project 3.4 takes advantage of recent advances in long-term optical recording of identified cells. This will allow us to make the first observation of the long-term stability of the stored sequence content replayed during SPW-Rs, as well as longitudinally identifying the network elements associated with this long-term replay, thereby providing important insight into the mechanisms of consolidation.