Storage and replay of information during SPW-Rs

在这个项目中，我们探讨的问题，如何在SPW-R重放的信息是 表示，如何以及在哪些子网络中，该信息可以在学习期间被编码， 以及导致海马存储信息的分子和网络机制 突触项目3.1试图开发一种方法，利用新的记忆来人工诱导记忆。 光遗传学方法允许在清醒行为动物中兴奋小群体神经元。 成功的一个衡量标准是人工记忆是否在SPW-R期间重放。的价值 这样一个系统将为研究记忆的生物物理学提供一个受控的系统 编码.项目3.2试图消除行为编码记忆。最近的研究表明， 记忆通过丰富的突触蛋白CaMKII以突触方式储存。这项工作是基于 显性负性CaMKII消除条件性位置回避的能力。我们将测试 CA 3区或DG区显性负性CaMK Ⅱ可消除海马系统重放信号的能力， 在SPW-R期间的内存。项目3.3探讨了空间信息如何 在内存中表示。一种观点认为空间记忆是一条连续的路径。 将测试备择假设，记忆可以有选择地代表奖励网站。 项目3.4利用了在识别细胞的长期光学记录方面的最新进展。 这将使我们能够对存储序列的长期稳定性进行首次观察 在SPW-R期间重放的内容，以及纵向标识网络元件 与这种长期重放相关，从而提供了重要的洞察机制， 合并。

In this project we explore question of how the information replayed during the SPW-R is represented, how and in which sub-networks this information may get encoded during learning, and the molecular and network mechanisms that lead to storage of information at hippocampal synapses. Project 3.1 seeks to develop a method for artificially inducing a memory using new optogenetic methods that allow small groups of neurons to be excited in awake behaving animals. A measure of success will be if the artificial memory is replayed during a SPW-R. The value of such a system will be that it will provide a controlled system for studying the biophysics of memory encoding. Project 3.2 seeks to erase a behaviorally encoded memory. Recent work suggests that memory is stored synaptically by the abundant synaptic protein, CaMKII. This work was based on the ability of dominant negative CaMKII to erase conditioned place avoidance. We will test whether dominant negative CaMKII in CA3 or DG can erase the ability of hippocampal system to replay a memory during the SPW-R. Project 3.3 explores the question of how spatial information is represented in memory. One view is that spatial memory is represented as a continuous path. We will test the alternative hypothesis that memory can be represented selectively by reward sites. Project 3.4 takes advantage of recent advances in long-term optical recording of identified cells. This will allow us to make the first observation of the long-term stability of the stored sequence content replayed during SPW-Rs, as well as longitudinally identifying the network elements associated with this long-term replay, thereby providing important insight into the mechanisms of consolidation.