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Continuous and Longitudinal Monitoring of Cerebral Blood Flow and Metabolism in Freely Moving Rodents

自由移动啮齿动物脑血流和代谢的连续和纵向监测

基本信息

批准号：
10204279
负责人：
Guoqiang Yu
金额：
$ 61.28万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10204279
关键词：
Adult Algorithms Anesthesia procedures Animal Behavior Animal Model Animals Behavioral Biological Markers Biomedical Research Blood Vessels Brain Brain Mapping Carbon Dioxide Cerebrovascular Circulation Cerebrovascular Disorders Cerebrum Complex Conscious Consumption Craniotomy Data Analyses Data Reporting Derivation procedure Devices Diffuse Elderly Electric Stimulation Electrodes Erythrocytes Eye Fiber Flowmetry Glucose Goals Head Hemoglobin Homeostasis Human Impaired cognition Infarction Inhalation Injections Intervention Investigation Ischemic Stroke Lasers Lead Light Measurement Measures Mental Depression Metabolic Metabolism Methods Modeling Monitor Motion Motor Movement Mus Neurons Neurosciences Neurosciences Research Operative Surgical Procedures Optics Outcome Output Oxygen Oxygen Consumption Oxygen saturation measurement Pathologic Performance Radiation Toxicity Rattus Rodent Rodent Model Scientist Sensory Sleep Sleep Disorders Source Stimulus Stress Stroke System Techniques Technology Testing Time Ultrasonography United States National Institutes of Health Validation Variant absorption attenuation awake base behavioral health behavioral study brain health brain tissue cerebral hemodynamics cerebral oxygenation cerebrovascular clinical application cognitive function cost cost effective cranium design detector flexibility human disease human subject innovation light scattering metabolic rate miniaturize multimodality nervous system disorder neural circuit neurovascular coupling new technology novel therapeutics optical sensor preterm newborn radiotracer relating to nervous system response sensor stroke model stroke outcome tool translational neuroscience

项目摘要

ABSTRACT Measurements of cerebral blood flow (CBF) and cerebral metabolic rate of oxygen consumption (CMRO2) in response to brain activity allows investigation of underlying excitatory and inhibitory neural responses and holds the key to optimizing novel therapies for neurological disorders and cerebrovascular diseases. Rodents (mice and rats) have been helping scientists investigate human diseases for well over a century and still make up 95% of the animal models used today. Major limitations with these rodent models are that cerebral hemodynamics is measured mostly in anesthetized animals and at limited time points. However, anesthesia impacts cerebral hemodynamics profoundly and interferes with studies of behavioral health, cognitive function, and sleep disorder. A few methods available for cerebral hemodynamic monitoring in conscious rodents require invasive craniotomy with installation of various opto-electrode/ultrasound probes onto the brain, which restrains animal’s head or body during measurements. Supported by NIH (R21), we have recently developed an innovative, noninvasive, low-cost, fiber-free, near-infrared diffuse speckle contrast flowmetry (DSCF) probe, which affixes on the heads of anesthetized rodents and awake humans for continuous monitoring of CBF. The goal of this proposal is to extend, optimize, and validate this novel technology toward a dual-wavelength, multi-channel, diffuse speckle contrast flow-oximetry (DSCFO) system for simultaneous regional mapping of CBF, cerebral oxygenation, and CMRO2 in conscious, freely moving rodents. DSCFO uses small near-infrared laser diodes at different wavelengths as point sources and a tiny CMOS camera as a 2D detector array to detect spatial dynamic light scattering by intrinsic motions of red blood cells (i.e., CBF) and light attenuations by oxy- and deoxy- hemoglobin absorptions ([HbO2] and [Hb]). Combination of CBF, [HbO2], and [Hb] enables derivation of CMRO2 based on established models. The 2D camera array enables 2D mapping of cerebral responses over two hemispheres and at different depths of the rodent head. Importantly, connections between the DSCFO probe and control unit are all electrical wires/cables (i.e., fiber-free), thereby offering the promise for continuous cerebral monitoring in freely moving subjects. After calibrating and optimizing the DSCFO system using head-simulating phantoms and validation in anesthetized rodents against established techniques, we will evaluate its performance for continuous and longitudinal cerebral monitoring in conscious, freely moving rodents with or without ischemic stroke insult. While we explore stroke-induced cerebral outcomes in this project, the DSCFO technology is applicable for studying other neurological disorders and cerebrovascular diseases. In combination with our ongoing R21 studies in human subjects, completion of this study in rodents will generate a unique noninvasive, fast, low-cost, multiscale, and multimodal brain mapping tool for both neuroscience research and clinical applications. Ultimately, the levels/variations and combination of CBF, [HbO2], [Hb], and CMRO2 may serve as biomarkers for assessing brain health and outcomes of cerebral pathological conditions and interventions.

摘要

项目成果

期刊论文数量（4）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Noncontact optical imaging of brain hemodynamics in preterm infants: a preliminary study.

DOI：
10.1088/1361-6560/abc5a7
发表时间：
2020-12-22
期刊：
Physics in medicine and biology
影响因子：
3.5
作者：
Abu Jawdeh EG;Huang C;Mazdeyasna S;Chen L;Chen L;Bada HS;Yu G
通讯作者：
Yu G

Intraoperative Optical and Fluorescence Imaging of Blood Flow Distributions in Mastectomy Skin Flaps for Identifying Ischemic Tissues.

乳房切除术皮瓣中血流分布的术中光学和荧光成像，以鉴定缺血组织。

DOI：
10.1097/prs.0000000000009333
发表时间：
2022-08-01
期刊：
Plastic and reconstructive surgery
影响因子：
3.6
作者：
通讯作者：

Noncontact Speckle Contrast Diffuse Correlation Tomography of Blood Flow Distributions in Burn Wounds: A Preliminary Study.

烧伤创面血流分布的非接触散斑对比漫射相关断层扫描：初步研究。

DOI：
10.1093/milmed/usz233
发表时间：
2020
期刊：
Military medicine
影响因子：
1.2
作者：
Zhao,Mingjun;Mazdeyasna,Siavash;Huang,Chong;Agochukwu-Nwubah,Nneamaka;Bonaroti,Alisha;Wong,Lesley;Yu,Guoqiang
通讯作者：
Yu,Guoqiang

Fractal Autoencoders for Feature Selection