National Resource for Aplysia

海兔国家资源

• 批准号: 10209624
• 负责人: MICHAEL C SCHMALE
• 金额: $ 25.88万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-05-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10209624
• 关键词: Address; Adolescent; Adult; Affect; Age; Aging; Animal Model; Animals; Aplysia; Aplysia species; Behavioral; Biological Metamorphosis; Biological Models; Biomedical Research; California; Cessation of life; Collection; Communities; Data; Databases; Deformity; Development; Disease; Education; Embryo; Fertilization; Gene Expression; Genome; Gills; Goals; Grant; Health; Health Status; Heart; Hemolymph; Infection; Investigation; Larva; Learning; Life; Measures; Memory; Methods; Mission; Monitor; Mucous body substance; Nervous System Physiology; Nervous system structure; Parents; Pathology; Prevalence; RNA; Reproducibility; Research; Resources; Sampling; Study models; Swab; Testing; Tissues; Universities; Viral; Viral Load result; Virus; Wild Animals; base; biosecurity; cohort; data mining; experimental study; germ free condition; hatching; improved; mortality; neurophysiology; non-invasive monitor; novel; parent grant; pathogen; prevent; programs; screening; senescence; transcriptome; transcriptome sequencing; transcriptomics; water sampling

Abstract Aplysia californica is a unique animal model contributing to groundbreaking discoveries in basic neurophysiology as well as the cellular basis of memory and learning. The genome of Aplysia has been sequenced and extensive genome and transcriptome resources are available, facilitating a wide variety of gene expression studies. Aplysia is also an outstanding model for studies of aging, with an annual lifecycle marked by significant behavioral, neurophysiological and transcriptomic hallmarks progressing to senescence and death. The overarching goals of the National Resource for Aplysia at the University of Miami (the Resource) are to produce and distribute consistently high-quality cultured California sea hares, A. californica, at all life stages for biomedical research and education, and to improve and expand the usefulness of this model system to the biomedical community. The goal of this application for supplemental support is to investigate the distribution and potential for pathology of a newly discovered, naturally occurring, nidovirus infection in Aplysia, Aplysia abyssovirus 1 (AAbV). Investigation of AAbV is directly relevant to the aims of the parent P40 grant: a key component of aim 1 of the parent grant is to monitor the health status of animals at the resource and to this end, limited monitoring of AAbV in hatchery animals has already been underway for the past year (with preliminary data presented here). Thus, the experiments proposed here clearly fit within the scope of aim 1 of the parent grant. The proposed studies would allow us to address important biosecurity concerns regarding the distribution and effects of AAbV that could not be supported by the parent grant alone. We propose two primary aims: 1. Development of improved testing and AAbV-free animals and 2. Assessment of pathology associated with AAbV. In aim 1 we will develop several methods for non-invasive monitoring of AAbV levels in Aplysia including hemolymph samples, gill swabs, fecal samples, and water samples. Theses methods will allow us to identify uninfected animals in cohorts while culling infected animals. An additional, major goal of this aim will be to create a consistent and reliable supply of AAbV-free (SPF) animals. Aim 2 will follow three discrete approaches to understand the pathology of AAbV infection by examining changes in gene expression and tissue ultrastructure associated with high viral loads. In this aim we will also investigate developmental impacts of AAbV on embryos and larvae.

摘要 加州无尾两栖动物是一种独特的动物模型，有助于基础研究的突破性发现。 神经生理学以及记忆和学习的细胞基础。Aesthesia的基因组已经 测序和广泛的基因组和转录组资源是可用的， 基因表达研究。失智症也是研究衰老的杰出模型， 生命周期以显著的行为、神经生理学和转录组学特征为标志， 走向衰老和死亡2010年亚洲国家资源的总体目标 迈阿密大学（资源）是生产和分发一贯的高品质培养 加州海兔A.加州，在所有生命阶段的生物医学研究和教育，并 改进和扩大该模型系统对生物医学界的有用性。这个目标 补充支持的申请是调查的分布和潜在的病理 新发现的，天然存在的，在Aabulsia，Aabulsia深海病毒1（AAbV）的巢状病毒感染。 对AAbV的调查与P40补助金的目标直接相关：目标1的关键组成部分 父母补助金的目的是监测资源中动物的健康状况，为此， 在过去的一年里，已经在孵化场动物中监测AAbV（初步 数据在这里）。因此，这里提出的实验显然符合《公约》目标1的范围。 家长补助拟议的研究将使我们能够解决重要的生物安全问题， AAbV的分布和影响，不能单独由母基金支持。我们提出 两个主要目标：1.开发改进的测试和无AAbV的动物和2.评估 与AAbV相关的病理学。在aim1中，我们将开发几种非侵入性监测的方法 包括血淋巴样品、鳃拭子、粪便样品和水样品。 这些方法将使我们能够在剔除受感染动物的同时，在队列中识别未感染动物。一个 此外，这一目标的主要目标将是创造一个一致和可靠的供应AAbV免费（SPF） 动物目的2将遵循三种不同的方法来理解AAbV感染的病理学， 检测与高病毒载量相关的基因表达和组织超微结构的变化。在这 我们还将研究AAbV对胚胎和幼虫发育的影响。