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Three-Dimensional UTE Magnetic Resonance Imaging of the Osteochondral Junction

骨软骨连接处的三维 UTE 磁共振成像

基本信息

批准号：
10207470
负责人：
Yajun Ma
金额：
$ 16.81万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-01 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10207470
关键词：
3-Dimensional Affect Age American Blood Vessels Bone Marrow Bone plates Cadaver Cartilage Clinical Clinical Treatment Cone Degenerative polyarthritis Diagnosis Disease Evaluation Extracellular Matrix Degradation Fatty acid glycerol esters Goals Histology Human Image Inflammation Joints Knee joint Ligaments Magnetic Resonance Imaging Marrow Metalloproteases Monitor Morphology Nerve Osteoclasts Pathogenesis Pathologic Patients Performance Physiologic pulse Play Preparation Protons Public Health Radial Recovery Reference Standards Relaxation Replacement Arthroplasty Reporting Resolution Role Sampling Scanning Scheme Series Signal Transduction Societies Source Specimen Stress System Techniques Tendon structure Time Tissues Up-Regulation Weight Weight-Bearing state Work arthropathies articular cartilage bone calcification clinical Diagnosis contrast imaging cost density healthy volunteer improved in vivo in vivo imaging interest knee replacement arthroplasty osteochondral tissue quantitative imaging radio frequency sex subchondral bone substantia spongiosa

项目摘要

Abstract Osteoarthritis (OA) is one of the most prevalent diseases in the US, affecting over 50 million Americans with an annual cost of more than $60 billion per year. The major public health issues associated with OA are likely to become even more important as our society ages. OA is considered a whole joint disease with pathologic changes that often involve all of the constituent joint tissues. Of increasing interest is the region of the osteochondral junction (OCJ), which encompasses the tissues between the deep uncalcified layers of cartilage and the marrow spaces of the trabecular bone. It includes the deep radial uncalcified cartilage, tidemark, calcified cartilage (CC), and subchondral bone (SCB) plate. While these tissues are avascular in the normal joint, in OA, osteoclasts are activated and form channels through the subchondral bone plate, allowing blood vessels and nerves to extend from the marrow into deep cartilage. This is associated with a cascade of abnormalities, including local inflammation and upregulation of metalloproteinase activity, extracellular matrix degradation, reduction of cartilage load-bearing capacity, and degenerative change. The OCJ may change dramatically in OA, and these changes may be relevant in its pathogenesis. Magnetic resonance imaging (MRI) is widely used to directly and non-invasively evaluate articular cartilage and plays an important role in the clinical diagnosis and treatment of OA. However, MRI of the OCJ region is difficult due to the short mean transverse relaxation times (i.e., short T2 or T2*) of deep radial uncalcified articular cartilage, calcified cartilage, and subchondral bone, which result in little or no signal when conventional pulse sequences are used. In this study we aim to develop 3D adiabatic inversion recovery prepared ultrashort echo time Cones (3D IR-Cones) techniques for direct volumetric morphological imaging and quantitative mapping of the OCJ, to validate the signal sources, and finally develop translational 3D IR-Cones techniques for OCJ imaging in vivo. In Aim 1 we will further develop and validate 3D IR-Cones techniques for morphological and quantitative imaging of the OCJ in osteochondral samples from cadaveric human knee joints (n=10). We will investigate the effect of spatial resolution, long T2 suppression, RF power, and gradient strengths, as well as T1, T2*, and proton density weightings in OCJ imaging on a 3T Bruker small-bore scanner and a clinical 3T scanner, respectively. We will correlate morphological and quantitative Cones findings with µCT and histology. In Aim 2 we will develop translational 3D IR-Cones techniques for morphological and quantitative imaging of the OCJ in knee joints of healthy volunteers (n=10) and patients subject to total knee replacement (n=10). The excised osteochondral samples from relatively normal (n=10) and more degenerated regions (n=10) will be re-scanned with clinical and 3D IR-Cones sequences, followed by µCT imaging and histology. In vivo and ex vivo 3D IR- Cones imaging of the OCJ will be compared and correlated with µCT imaging and histology.

摘要骨关节炎（OA）是美国最常见的疾病之一，影响超过5000万美国人每年的成本超过600亿美元。与OA相关的主要公共卫生问题是随着我们社会的老龄化，它可能变得更加重要。OA被认为是一种整体关节疾病，病理变化，往往涉及所有的组成关节组织。越来越令人感兴趣的是骨软骨交界处（OCJ），它包括深未钙化层之间的组织，软骨和骨小梁的骨髓间隙。它包括深层桡骨未钙化软骨，潮标、钙化软骨（CC）和软骨下骨（SCB）板。虽然这些组织在血管中是无血管的，在正常关节中，在OA中，破骨细胞被激活并形成穿过软骨下骨板的通道，血管和神经从骨髓延伸到深层软骨。这与一系列的异常，包括局部炎症和金属蛋白酶活性上调，细胞外基质降解、软骨承重能力降低和退行性变化。OCJ可能会改变这些变化可能与其发病机制有关。磁共振成像 (MRI)广泛用于直接和非侵入性地评价关节软骨， OA的临床诊断和治疗。然而，由于短的平均值，OCJ区域的MRI是困难的横向弛豫时间（即，短T2或T2*）的深桡骨未钙化关节软骨，钙化软骨，和软骨下骨，当使用常规脉冲序列时，这导致很少或没有信号。在这项研究中，我们的目标是发展三维绝热反演恢复制备超短回波时间锥（三维 IR-Cones）技术进行直接体积形态学成像和OCJ的定量映射，验证信号源，并最终开发用于OCJ体内成像的平移3D IR-Cones技术。在目标1中，我们将进一步开发和验证用于形态和定量的3D IR圆锥技术来自尸体人膝关节的骨软骨样品中的OCJ成像（n=10）。我们将调查空间分辨率、长T2抑制、RF功率和梯度强度以及T1、T2* 和在3 T Bruker小口径扫描仪和临床3 T扫描仪上的OCJ成像中的质子密度加权，分别我们将把形态学和定量锥细胞发现与µCT和组织学相关联。在目标2中我们将开发用于OCJ形态学和定量成像的平移3D IR锥技术，健康志愿者（n=10）和全膝关节置换术患者（n=10）的膝关节。切除的将重新扫描相对正常（n=10）和更严重退化区域（n=10）的骨软骨样本临床和3D IR-Cones序列，随后进行µCT成像和组织学检查。体内和离体3D IR- OCJ的锥体成像将与µCT成像和组织学进行比较和关联。

项目成果

期刊论文数量（32）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The effect of cartilage dehydration and rehydration on quantitative ultrashort echo time biomarkers.

DOI：
10.21037/qims-23-359
发表时间：
2023-10-01
期刊：
Quantitative imaging in medicine and surgery
影响因子：
2.8
作者：
通讯作者：

Assessment of mechanical properties of articular cartilage with quantitative three-dimensional ultrashort echo time (UTE) cones magnetic resonance imaging.

DOI：
10.1016/j.jbiomech.2020.110085
发表时间：
2020-12-02
期刊：
Journal of biomechanics
影响因子：
2.4
作者：
Namiranian B;Jerban S;Ma Y;Dorthe EW;Masoud-Afsahi A;Wong J;Wei Z;Chen Y;D'Lima D;Chang EY;Du J
通讯作者：
Du J

AcidoCEST-UTE MRI Reveals an Acidic Microenvironment in Knee Osteoarthritis.

AcidoCEST-UTE MRI 显示膝骨关节炎的酸性微环境。

DOI：
10.3390/ijms23084466
发表时间：
2022-04-18
期刊：
International journal of molecular sciences
影响因子：
5.6
作者：
通讯作者：

High contrast cartilaginous endplate imaging using a 3D adiabatic inversion-recovery-prepared fat-saturated ultrashort echo time (3D IR-FS-UTE) sequence.

DOI：
10.1002/nbm.4579
发表时间：
2021-10
期刊：
NMR in biomedicine
影响因子：
2.9
作者：
Lombardi AF;Wei Z;Wong J;Carl M;Lee RR;Wallace M;Masuda K;Chang EY;Du J;Ma YJ
通讯作者：
Ma YJ

Evaluation of enzymatic proteoglycan loss and collagen degradation in human articular cartilage using ultrashort echo time-based biomarkers: A feasibility study.