Three-Dimensional UTE Magnetic Resonance Imaging of the Osteochondral Junction

骨软骨连接处的三维 UTE 磁共振成像

• 批准号: 10058140
• 负责人: Yajun Ma
• 金额: $ 20.79万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10058140
• 关键词: 3-Dimensional; Affect; Age; American; Blood Vessels; Bone Marrow; Bone plates; Cadaver; Cartilage; Clinical; Clinical Treatment; Cone; Degenerative polyarthritis; Diagnosis; Disease; Evaluation; Extracellular Matrix Degradation; Fatty acid glycerol esters; Goals; Histology; Human; Image; Inflammation; Joints; Knee joint; Ligaments; Magnetic Resonance Imaging; Marrow; Metalloproteases; Monitor; Morphology; Nerve; Osteoclasts; Pathogenesis; Pathologic; Patients; Performance; Physiologic pulse; Play; Preparation; Protons; Public Health; Radial; Recovery; Reference Standards; Relaxation; Replacement Arthroplasty; Reporting; Resolution; Role; Sampling; Scanning; Scheme; Series; Signal Transduction; Societies; Source; Specimen; Stress; System; Techniques; Tendon structure; Time; Tissues; Up-Regulation; Weight; Weight-Bearing state; Work; arthropathies; articular cartilage; bone; calcification; clinical Diagnosis; contrast imaging; cost; density; healthy volunteer; improved; in vivo; in vivo imaging; interest; knee replacement arthroplasty; osteochondral tissue; quantitative imaging; radio frequency; sex; subchondral bone; substantia spongiosa

Abstract Osteoarthritis (OA) is one of the most prevalent diseases in the US, affecting over 50 million Americans with an annual cost of more than $60 billion per year. The major public health issues associated with OA are likely to become even more important as our society ages. OA is considered a whole joint disease with pathologic changes that often involve all of the constituent joint tissues. Of increasing interest is the region of the osteochondral junction (OCJ), which encompasses the tissues between the deep uncalcified layers of cartilage and the marrow spaces of the trabecular bone. It includes the deep radial uncalcified cartilage, tidemark, calcified cartilage (CC), and subchondral bone (SCB) plate. While these tissues are avascular in the normal joint, in OA, osteoclasts are activated and form channels through the subchondral bone plate, allowing blood vessels and nerves to extend from the marrow into deep cartilage. This is associated with a cascade of abnormalities, including local inflammation and upregulation of metalloproteinase activity, extracellular matrix degradation, reduction of cartilage load-bearing capacity, and degenerative change. The OCJ may change dramatically in OA, and these changes may be relevant in its pathogenesis. Magnetic resonance imaging (MRI) is widely used to directly and non-invasively evaluate articular cartilage and plays an important role in the clinical diagnosis and treatment of OA. However, MRI of the OCJ region is difficult due to the short mean transverse relaxation times (i.e., short T2 or T2*) of deep radial uncalcified articular cartilage, calcified cartilage, and subchondral bone, which result in little or no signal when conventional pulse sequences are used. In this study we aim to develop 3D adiabatic inversion recovery prepared ultrashort echo time Cones (3D IR-Cones) techniques for direct volumetric morphological imaging and quantitative mapping of the OCJ, to validate the signal sources, and finally develop translational 3D IR-Cones techniques for OCJ imaging in vivo. In Aim 1 we will further develop and validate 3D IR-Cones techniques for morphological and quantitative imaging of the OCJ in osteochondral samples from cadaveric human knee joints (n=10). We will investigate the effect of spatial resolution, long T2 suppression, RF power, and gradient strengths, as well as T1, T2*, and proton density weightings in OCJ imaging on a 3T Bruker small-bore scanner and a clinical 3T scanner, respectively. We will correlate morphological and quantitative Cones findings with µCT and histology. In Aim 2 we will develop translational 3D IR-Cones techniques for morphological and quantitative imaging of the OCJ in knee joints of healthy volunteers (n=10) and patients subject to total knee replacement (n=10). The excised osteochondral samples from relatively normal (n=10) and more degenerated regions (n=10) will be re-scanned with clinical and 3D IR-Cones sequences, followed by µCT imaging and histology. In vivo and ex vivo 3D IR- Cones imaging of the OCJ will be compared and correlated with µCT imaging and histology.

抽象的 骨关节炎（OA）是美国最普遍的疾病之一，影响了超过5000万美国人 每年的年成本超过600亿美元。与OA相关的主要公共卫生问题是 随着我们的社会年龄，可能变得更加重要。 OA被认为是整个关节疾病， 通常涉及所有组成关节组织的病理变化。兴趣越来越大的是 骨软骨结（OCJ），涵盖了深度未添加层之间的组织 软骨和小梁骨的骨髓空间。它包括深度径向未归化的软骨， Tidemark，计算的软骨（CC）和软骨下骨（SCB）板。这些组织在 正常的关节，在OA中，将破骨细胞激活并通过软骨下骨板形成通道，从而允许 血管和神经从骨髓延伸到深软骨。这与 异常，包括局部注射和金属蛋白酶活性的上调，细胞外基质 降解，减少软骨负荷容量和退化性变化。 OCJ可能会改变 磁共振成像 （MRI）广泛用于直接和非侵入性评估关节软骨，并在 OA的临床诊断和治疗。但是，由于平均值短，OCJ区域的MRI很困难 深度径向未取代关节软骨的横向松弛时间（即短T2或T2*），钙化软骨， 和软骨下骨，当使用常规脉冲序列时，几乎没有信号。 在这项研究中，我们旨在开发3D绝热恢复恢复制备的超短回声时间锥（3D IR-CONE）技术，用于直接体积形态成像和OCJ定量映射的技术 验证信号源，并最终开发用于体内OCJ成像的转移3D IR-CONE技术。 在AIM 1中，我们将进一步开发和验证3D IR-CONES技术以形态和定量 来自尸体人膝关节的骨软骨样品中OCJ的成像（n = 10）。我们将调查 空间分辨率，长T2抑制，RF功率和梯度强度以及T1，T2*和 3T布鲁克小孔扫描仪和临床3T扫描仪上的OCJ成像中的质子密度权重， 分别。我们将将形态学和定量锥发现与µCT和组织学相关。在目标2中 我们将开发转化3D IR-CONES技术，用于OCJ的形态和定量成像 健康志愿者的膝关节（n = 10）和患者进行总膝关节置换（n = 10）。兴奋 将重新扫描来自相对正常（n = 10）和更多退化区域（n = 10）的骨软骨样品 具有临床和3D IR-CONE序列，然后进行µCT成像和组织学。体内和ex vivo 3d ir- OCJ的锥体成像将被比较并与µCT成像和组织学相关。