Molecular Mechanisms Regulating Epithelial Cell Apical Polarity and Ciliogenesis

项目摘要 上皮细胞表现出显着的顶基底亚细胞极性，这是至关重要的正常功能， 上皮组织，并允许上皮细胞形成一个专门的顶端结构，如顶端初级纤毛。 最后，上皮细胞与邻近细胞协调其极化以形成顶端腔，这是上皮细胞分化的关键步骤。 肾结构的建立，从而功能。因此，破坏细胞功能的突变， 顶基底极化和顶纤毛形成所必需的分子导致多种肾病， 例如多囊肾病（PKD）。因此，本项目的总体目标是描述 肾脏顶端腔形成和纤毛发生的分子机制。为此，我们设计了 三个具体目标。在aim#1中，我们将分析研究决定在哪里的分子机制。 以及顶端腔何时形成以及如何与纤毛发生协调。具体来说，我们将测试 新鉴定的极性调节因子Tbc 1d 1在肾脏发育过程中介导管腔形成。在 aim#2我们将剖析顶端腔发生和初级纤毛形成/信号传导之间的协调。 我们最近鉴定了Rab 19作为Tbc 1d 1结合蛋白，并表明Rab 19是纤毛所必需的 阵因此，我们将研究Rab 19在介导纤毛形成和靶向纤毛驻留中的作用。 proteins.最后，在目标3中，我们将测试分析介导肾腔形成的机制， 使用斑马鱼的体内纤毛发生。该项目的完成将提供一个新的见解，了解 正常人肾上皮细胞极化和纤毛形成的分子机制和调控 上皮组织形态发生和疾病。

Project Summary Epithelial cells exhibit remarkable apicobasal subcellular polarity that is essential for the proper functioning of epithelial tissues and allows epithelial cells to form a specialized apical structures, such as apical primary cilia. Finally, epithelial cells coordinate their polarization with neighboring cells to form an apical lumen, a key step in the establishment of renal architecture, and thereby function. Accordingly, mutations that disrupt function of molecules necessary for apicobasal polarization and apical cilia formation result in a variety of renal disorders, such as polycystic kidney disease (PKD). Thus, the overarching goal of this project is to characterize the molecular machinery coordinating apical lumen formation and ciliogenesis in kidney. To that end, we designed three specific aims. In the aim#1 we will analyze investigate the molecular machinery that determines where and when apical lumen forms and how that is coordinated with ciliogenesis. Specifically, we will test the role of newly identified polarity regulator Tbc1d1 in mediating lumenogenesis during kidney development. In the aim#2 we will dissect the coordination between the apical lumenogenesis and primary cilia formation/signaling. We recently identified Rab19 as Tbc1d1 binding protein and have shown that Rab19 is required for cilia formation. Thus, we will investigate the role of Rab19 in mediating cilia formation and targeting of cilia resident proteins. Finally, in aim#3 we will test dissect the mechanisms mediating kidney lumenogenesis and ciliogenesis in vivo using zebrafish. Completion of this project will provide a novel insight in understanding the molecular machinery and regulation of kidney epithelial cell polarization and cilia formation during normal epithelial tissue morphogenesis and in disease.