Photoreversible hydrogels to study stem cell memory and fate

用于研究干细胞记忆和命运的光可逆水凝胶

• 批准号: DP210100393
• 负责人: A/Prof Jessica Frith
• 金额: $ 29万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2021
• 资助国家: 澳大利亚
• 起止时间: 2021-01-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP210100393
• 关键词: Photoreversible; hydrogels; study; stem; cell

This project will develop materials whose stiffness can be reversibly increased and decreased by the simple application of light, and use these to build knowledge of how stem cell fate is regulated. The influence of mechanical cues on the structure and organisation of the nucleus will be determined. Expected outcomes are new synthetic and light-reversible culture materials, and fundamental insights into how forces change the nucleus to alter stem cell aging and fate. The findings will provide critical information required for the future development of assays to measure cell potency and instructive biomaterials to drive stem cell expansion and tissue-regeneration and will have impact by underpinning future advances in stem cell technologies.

该项目将开发其刚度可以通过简单的光应用可逆地增加和减少的材料，并使用这些材料来建立干细胞命运如何调节的知识。将确定机械线索对细胞核结构和组织的影响。预期成果是新的合成和光可逆的培养材料，以及对力如何改变细胞核以改变干细胞衰老和命运的基本见解。这些发现将为未来开发测量细胞潜能的检测方法和指导性生物材料以驱动干细胞扩增和组织再生提供所需的关键信息，并将通过支持干细胞技术的未来进展产生影响。