Histology, Biochemistry and Molecular Imaging (HBMI) Core

罗切斯特大学组织、生物化学和分子成像(HBMI)核心资源- 骨、肌肉和整形外科研究中心(ROCSTARR)将提供组织病理学， 组织形态计量学、生物化学、细胞和分子成像和分析服务以及最先进的 支持肌肉骨骼研究中心临床和基础研究的资源和培训 (CMSR)在博伊斯和阿诺里克博士的领导下。它有4个研究和服务分中心：1)组织学 和免疫组织化学；2)显微镜、组织形态计量学和三维组织清除/成像；3)生物化学， 细胞、分子和单细胞基因组学；以及4)小动物手术和支持。熟练的次核心 ROCSTARR顾问和实验室技术人员将提供使用尖端仪器和 每个子核心中的技术。核心将整合这些服务和资源，并推进 了解生化和分子信号以及细胞对炎症、再生的反应 并影响MSK组织的修复。它还将加快基础研究成果转化为临床实践的进程。 核心将开发新的技术，将继续改变对MSK开发和 动物和人类的疾病过程。HBMI核心内部服务的扩展和整合 将进一步提高生产率，提高核心的效率，使组织之间的翻译 和组织形态计量学数据以及肌肉骨骼潜在的细胞和分子机制 并加快CMSR范围内所有资助项目的研究步伐。的具体目标 ROCSTARR所有调查人员及其合作者的核心是：1)提供经济高效和高效率的- 高质量的组织学、IHC、成像和组织形态计量学服务；2)为ROCSTARR教职员工和他们的 合作者可以访问最先进的分子生化、细胞和分子分析，包括新的 分离细胞和生物信息学管道上的单细胞转录平台，并帮助培训学生 和博士后使用HBMI标准操作程序；3)为我们的 通过整合员工和ROCSTARR专家教师作为专业小组的顾问，进行新的/早期调查 提供关于尖端组织学、影像、生化和 分子技术；以及4)推动MSK基础、翻译和临床研究：a)提供完整的 玻片成像技术及MSK组织形态计量学参数自动分析的研究进展 使用Visiopamm图像分析软件；b)扩大对多路组织学平台的访问(MASS 细胞术和免疫荧光)；c)应用新型深层组织高空间成像平台，包括 组织清除、电子显微镜和MSK组织的3D重建；d)制定单个 细胞转录学，高质量核酸的分离，以及用于高质量数据生成和 分析；以及e)整合组织组织学和MSK组织的10倍维西姆单细胞转录分析。

The Histology, Biochemistry, and Molecular Imaging (HBMI) Core of the University of Rochester Resource- based Center for Bone, Muscle and Orthopaedic Research (ROCSTARR) will provide histopathology, histomorphometry, biochemistry, and cellular and molecular imaging and analysis services and state-of-the-art resources and training to support clinical and basic research in the Center for Musculoskeletal Research (CMSR) under the leadership of Drs. Boyce and Anolik. It has 4 research and service Sub-Cores: 1) Histology & Immunohistochemistry; 2) Microscopy, Histomorphometry & 3-D Tissue Clearing/Imaging; 3) Biochemistry, Cellular, Molecular, & Single Cell Omics; and 4) Small Animal Surgery and Support. Skilled Sub-Core ROCSTARR advisors and lab technicians will provide training in use of sophisticated instruments and techniques within each Sub-Core. The Core will integrate these services and resources and advance understanding of how biochemical and molecular signals and cellular responses to inflammation, regeneration and repair affect MSK tissues. It will also accelerate advances in basic research findings to clinical practice. The Core will develop new techniques that will continue to transform understanding of MSK developmental and disease processes in animals and humans. The expansion and integration of services within the HBMI Core will further increase productivity, enhance the efficiency of the Core, enable the translation between histologic and histomorphometric data and the underlying cellular and molecular mechanisms in musculoskeletal diseases and accelerate the pace of research for all funded projects within the CMSR. The Specific Aims of the Core for all ROCSTARR investigators and their collaborators are to: 1) provide cost-efficient and high- quality histology, IHC, imaging, and histomorphometry services; 2) provide ROCSTARR faculty and their collaborators access to state-of-the-art molecular biochemical, cellular, and molecular analytics, including new single cell transcriptomic platforms on dissociated cells and bioinformatics pipelines, and to help train students and post-docs to use HBMI standard operating procedures; 3) provide expertise and mentorship to our New/Early Stage Investigators by integrating staff and expert ROCSTARR faculty as Advisors in specialty Sub- Cores to offer conceptual and technical guidance on cutting edge histologic, imaging, biochemical, and molecular technologies; and 4) advance MSK basic, translational and clinical research by: a) providing whole slide imaging technology and developing automated analysis of histomorphometric parameters of MSK tissues with Visiopharm image analysis software; b) expanding access to multiplexed histology platforms (mass cytometry and immunofluorescence); c) applying novel deep tissue high-spatial imaging platforms, including tissue clearing, electron microscopy and 3D reconstruction to MSK tissue; d) developing protocols for single cell transcriptomics, isolation of high quality nucleic acids, and a pipeline for high quality data generation and analysis; and e) integrating tissue histology and 10X Visium single cell transcriptomic analysis of MSK tissue.