Histology, Biochemistry and Molecular Imaging (HBMI) Core

组织学、生物化学和分子成像 (HBMI) 核心

• 批准号: 10232835
• 负责人: Brendan F Boyce
• 金额: $ 25.92万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10232835
• 关键词: 3-Dimensional; Achievement; Affect; Animals; Basic Science; Biochemical; Biochemistry; Cartilage; Cells; Clinical Research; Computer software; Cytometry; Data; Electron Microscopy; Faculty; Funding; Generations; Goals; Grant; Histologic; Histology; Histopathology; Human; Image; Image Analysis; Imaging technology; Immunofluorescence Immunologic; Immunohistochemistry; Individual; Inflammation; Infrastructure; Leadership; Medicine; Mentors; Mentorship; Microscopy; Molecular; Molecular Analysis; Muscle; Musculoskeletal; Musculoskeletal Diseases; National Institute of Arthritis and Musculoskeletal and Skin Diseases; Natural regeneration; Nucleic Acids; Operative Surgical Procedures; Orthopedics; Pathway interactions; Population; Postdoctoral Fellow; Procedures; Process; Productivity; Protocols documentation; Publications; Research; Research Personnel; Resources; Secure; Services; Signal Transduction; Synovial Membrane; Systems Analysis; Techniques; Technology; Tendon structure; Tissue imaging; Tissues; Training; Translational Research; Translations; United States National Institutes of Health; Universities; automated analysis; base; bioinformatics pipeline; bone; career development; cellular imaging; clinical practice; clinical translation; cost efficient; developmental disease; experience; histological image; imaging platform; improved; innovation; instrument; interdisciplinary approach; medical specialties; molecular imaging; musculoskeletal imaging; new technology; novel; programs; ranpirnase; reconstruction; repaired; response; single cell analysis; skills; student training; success; tissue processing; training opportunity; transcriptomics; whole slide imaging

The Histology, Biochemistry, and Molecular Imaging (HBMI) Core of the University of Rochester Resource- based Center for Bone, Muscle and Orthopaedic Research (ROCSTARR) will provide histopathology, histomorphometry, biochemistry, and cellular and molecular imaging and analysis services and state-of-the-art resources and training to support clinical and basic research in the Center for Musculoskeletal Research (CMSR) under the leadership of Drs. Boyce and Anolik. It has 4 research and service Sub-Cores: 1) Histology & Immunohistochemistry; 2) Microscopy, Histomorphometry & 3-D Tissue Clearing/Imaging; 3) Biochemistry, Cellular, Molecular, & Single Cell Omics; and 4) Small Animal Surgery and Support. Skilled Sub-Core ROCSTARR advisors and lab technicians will provide training in use of sophisticated instruments and techniques within each Sub-Core. The Core will integrate these services and resources and advance understanding of how biochemical and molecular signals and cellular responses to inflammation, regeneration and repair affect MSK tissues. It will also accelerate advances in basic research findings to clinical practice. The Core will develop new techniques that will continue to transform understanding of MSK developmental and disease processes in animals and humans. The expansion and integration of services within the HBMI Core will further increase productivity, enhance the efficiency of the Core, enable the translation between histologic and histomorphometric data and the underlying cellular and molecular mechanisms in musculoskeletal diseases and accelerate the pace of research for all funded projects within the CMSR. The Specific Aims of the Core for all ROCSTARR investigators and their collaborators are to: 1) provide cost-efficient and high- quality histology, IHC, imaging, and histomorphometry services; 2) provide ROCSTARR faculty and their collaborators access to state-of-the-art molecular biochemical, cellular, and molecular analytics, including new single cell transcriptomic platforms on dissociated cells and bioinformatics pipelines, and to help train students and post-docs to use HBMI standard operating procedures; 3) provide expertise and mentorship to our New/Early Stage Investigators by integrating staff and expert ROCSTARR faculty as Advisors in specialty Sub- Cores to offer conceptual and technical guidance on cutting edge histologic, imaging, biochemical, and molecular technologies; and 4) advance MSK basic, translational and clinical research by: a) providing whole slide imaging technology and developing automated analysis of histomorphometric parameters of MSK tissues with Visiopharm image analysis software; b) expanding access to multiplexed histology platforms (mass cytometry and immunofluorescence); c) applying novel deep tissue high-spatial imaging platforms, including tissue clearing, electron microscopy and 3D reconstruction to MSK tissue; d) developing protocols for single cell transcriptomics, isolation of high quality nucleic acids, and a pipeline for high quality data generation and analysis; and e) integrating tissue histology and 10X Visium single cell transcriptomic analysis of MSK tissue.

罗切斯特大学的组织学、生物化学和分子成像（HBMI）核心资源- 的骨骼，肌肉和矫形研究中心（ROCSTARR）将提供组织病理学， 组织形态学、生物化学、细胞和分子成像和分析服务以及最先进的技术 资源和培训，以支持肌肉骨骼研究中心的临床和基础研究 （CMSR）在博伊斯和Anolik博士的领导下。它有4个研究和服务子核心：1）组织学 和免疫组织化学; 2）显微镜、组织形态测定和3-D组织清除/成像; 3）生物化学， 细胞，分子和单细胞组学;和4）小动物手术和支持。熟练次核心 ROCSTARR顾问和实验室技术人员将提供使用复杂仪器的培训， 每个子核心中的技术。核心将整合这些服务和资源， 了解生化和分子信号以及细胞对炎症、再生的反应 和修复影响MSK组织。它还将加速基础研究成果向临床实践的进展。 核心将开发新的技术，将继续改变对MSK发展和 动物和人类的疾病过程。HBMI核心服务的扩展和整合 将进一步提高生产力，提高核心的效率，使组织学之间的翻译， 和组织形态计量学数据以及肌肉骨骼中潜在的细胞和分子机制 疾病，并加快CMSR内所有资助项目的研究步伐。的具体目标 本研究中心的研究人员与合作者的核心是：一、提供高效益、高效率的服务; 高质量的组织学、免疫组化、影像学和组织形态学服务; 2）提供ROCSTARR教师和他们的 合作者获得最先进的分子生物化学，细胞和分子分析，包括新的 单细胞转录组学平台上的解离细胞和生物信息学管道，并帮助培训学生 和博士后使用HBMI标准操作程序; 3）提供专业知识和指导，以我们的 新的/早期阶段的研究人员通过整合工作人员和专家ROCSTARR教师作为专业小组的顾问， 核心，提供尖端组织学，影像学，生化， 分子技术;和4）推进MSK基础，转化和临床研究：a）提供整个 载玻片成像技术和开发MK组织组织形态计量参数的自动化分析 使用Visiopharm图像分析软件; B）扩大对多路复用组织学平台（mass c）应用新型深部组织高空间成像平台，包括 组织清除、电子显微镜检查和MSK组织的3D重建; d）开发用于单个 细胞转录组学，高质量核酸的分离，以及高质量数据生成的管道， e）整合MSK组织的组织学和10 X Visium单细胞转录组学分析。