Smart skin grafts for quantitative assessment and treatment of diabetic wounds

用于定量评估和治疗糖尿病伤口的智能植皮

• 批准号: 10218396
• 负责人: Huanyu Cheng
• 金额: $ 61.96万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-18 至 2024-09-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10218396
• 关键词: Adhesions; Affect; Amputation; Animals; Anti-Bacterial Agents; Antifungal Agents; Bacterial Infections; Caring; Chronic; Citrates; Clinical; Clinical Trials; Debridement; Devices; Diabetes Mellitus; Diabetic Foot; Diabetic Foot Ulcer; Electric Stimulation; Electrodes; Electronics; Environment; Evaluation; Excision; Gold; Grant; Health Care Costs; Hydrogels; Infection; Infection Control; Inflammation; Injectable; Intervention; Limb structure; Lower Extremity; Measures; Medical; Methods; Microbiology; Monitor; Patients; Performance; Plantar Ulcers; Process; Provider; Public Health; Quality of life; Research Personnel; Research Proposals; Skin; Skin graft; Temperature; Therapeutic; Tissue Engineering; Tissues; Trace Elements; Ulcer; United States; Visualization; Wound models; Yang; Zinc; base; cell motility; chronic wound; clinically relevant; design; diabetic ulcer; electric field; experience; fungus; healing; improved; in vivo; interactive feedback; keratinocyte; migration; next generation; point of care; pressure; prevent; sensor; wound; wound care; wound closure; wound dressing; wound environment; wound healing; wound treatment

Project Summary Chronic, difficult-to-heal wounds affect the lives of more than 25 million people with diabetes in the United States, leading to increased healthcare costs and decreased quality of life. Patients with late stage diabetic foot ulcers often progress to amputation for a gangrenous lower extremity limb. Therefore, early stage medical intervention is critical for diabetic foot care. Because of pressure relief, the total-contact cast is the gold standard and most widely used method for healing the most common type of plantar ulcerations (grade 1 ulcers). However, the cast needs to be changed periodically for wound inspection and evaluation, which is burdensome to both patients and providers. Therefore, it is desirable to continuously monitor wounds without direct visualization. Furthermore, because the endogenous electric field is askew or absent in chronic diabetic wounds, cell migration is impeded, often rendering standard wound care inadequate. By mimicking the endogenous electric field, electrical stimulation has been shown in several clinical trials to enhance healing and closure. While many smart wound dressings have been developed to monitor the wound environment, they need regular changes, impeding wound healing. Although skin grafts eliminate the need for removal, they lack sensing capabilities and cannot mitigate infections. Here, the investigators propose to develop and validate paradigm-shifting fully resorbable smart tissue-engineering skin grafts for diabetic wound care. The fully resorbable smart tissue-engineering skin grafts could quantitatively assess the wound environment and concurrently deliver electrical stimulation for enhanced wound healing. The PI Dr. Cheng recently developed skin-conformal electronics to continuously monitor the temperature and moisture levels of the wound, along with the fabrication of biodegradable sensors and electrodes with an essential trace element of zinc on a biodegradable substrate. The anti-bacterial/anti-fungal hydrogels recently developed in co-I Dr. Yang’s lab have been shown to have high wet adhesion for sutureless wound closure with nearly no chronic inflammation. As a clinical microbiologist, co-I Dr. Craft with expertise in microbiology will help guide the clinical relevance. In addition to co-I Dr. Ravnic’s extensive experiences on animal and patient studies with infected diabetic wounds, MD Ulbrecht will also contribute his expertise in diabetic foot care. The investigators will first develop and validate Zn-based temperature/moisture sensors on the anti-bacterial/anti- fungal bioadhesive substrate. After integrating the Zn-based electrode on the bioadhesive, the investigators will investigate the therapeutic function of the smart skin graft via electrical stimulation because of infection reduction and electric field-facilitated migration and proliferation for diabetic wound care. The smart skin graft will substantially improve wound care by quantitative assessment and enhanced treatment of chronic wounds, which can result in next-generation wound care devices to replace the existing wound dressings or skin grafts.

项目摘要 在美国，慢性、难以愈合的伤口影响着超过2500万糖尿病患者的生活， 导致医疗费用增加和生活质量下降。晚期糖尿病足溃疡患者 下肢坏疽后往往要截肢。因此，早期医疗干预 对于糖尿病足护理至关重要。由于压力释放，全接触铸造是黄金标准， 广泛用于治愈最常见类型的足底溃疡（1级溃疡）的方法。但 需要定期更换石膏以进行伤口检查和评估，这对两个患者来说都是负担 和供应商。因此，期望连续监测伤口而不直接可视化。 此外，由于内源性电场在慢性糖尿病伤口中歪斜或缺失， 这常常使标准的伤口护理不充分。通过模拟内源性电场， 电刺激已经在几个临床试验中显示出增强愈合和闭合。虽然许多聪明的 伤口敷料已经被开发用于监测伤口环境，它们需要定期更换，阻碍伤口愈合。 伤口愈合虽然皮肤移植消除了移除的需要，但它们缺乏感知能力，并且不能 减轻感染。在这里，研究人员建议开发和验证范式转移完全可吸收 用于糖尿病伤口护理的智能组织工程皮肤移植物。可完全吸收的智能组织工程皮肤 移植物可以定量地评估伤口环境并同时递送电刺激， 促进伤口愈合。 PI郑博士最近开发了皮肤适形电子设备，以持续监测温度， 伤口的湿度水平，沿着可生物降解的传感器和电极的制造， 在可生物降解的基质上的必需微量元素锌。抗细菌/抗真菌水凝胶最近 杨博士的实验室开发的无缝合伤口缝合线具有高湿粘附力， 几乎没有慢性炎症。作为一名临床微生物学家，具有微生物学专业知识的副I Craft博士将提供帮助 指导临床相关性。Ravnic博士除了在动物和患者研究方面的丰富经验外， 对于感染的糖尿病伤口，医学博士Ulbrecht也将贡献他在糖尿病足护理方面的专业知识。的 研究人员将首先开发和验证锌基温度/湿度传感器的抗菌/抗- 真菌生物粘附基质。在将锌基电极整合到生物粘附剂上后，研究人员将 通过电刺激研究智能皮肤移植物的治疗功能，因为感染减少 和电场促进的迁移和增殖用于糖尿病伤口护理。智能皮肤移植将 通过定量评估和增强慢性伤口治疗显著改善伤口护理， 可以产生下一代伤口护理装置以取代现有的伤口敷料或皮肤移植物。

项目成果

A wireless, solar-powered, optoelectronic system for spatial restriction-free long-term optogenetic neuromodulations.

• DOI: 10.1126/sciadv.adi8918
• 发表时间: 2023-09-29
• 期刊: SCIENCE ADVANCES
• 影响因子: 13.6
• 作者: Park, Jaejin;Kim, Kyubeen;Kim, Yujin;Kim, Tae Soo;Min, In Sik;Li, Bowen;Cho, Young Uk;Lee, Chanwoo;Lee, Ju Young;Gao, Yuyan;Kang, Kyowon;Kim, Do Hyeon;Choi, Won Jun;Shin, Hyun-Beom;Kang, Ho Kwan;Song, Young Min;Cheng, Huanyu;Cho, Il-Joo;Yu, Ki Jun
• 通讯作者: Yu, Ki Jun

Highly Sensitive Wearable Sensor Based on (001)-Orientated TiO2 for Real-Time Electrochemical Detection of Dopamine, Tyrosine, and Paracetamol.

• DOI: 10.1002/smll.202312238
• 发表时间: 2024-02
• 期刊: Small
• 影响因子: 13.3
• 作者: Tianyao Zhang;Jia Zhu;Maowen Xie;Ke Meng;Guang Yao;T. Pan;Min Gao;H. Cheng;Yuan Lin