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Gas Chromatography-Molecular Rotational Resonance Spectrometer

气相色谱-分子旋转共振光谱仪

基本信息

批准号：
10218225
负责人：
Justin Lindsay Neill
金额：
$ 60.4万
依托单位：
BRIGHTSPEC, INC.
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10218225
关键词：
3-Dimensional Adoption Amino Acids Amino Sugars Analytical Chemistry Applications Grants Area Award Basic Science Benchmarking Biological Biological Markers Biological Monitoring Complement Complex Mixtures Computer software Coupling Data Development Environmental Monitoring Environmental Pollutants Environmental Pollution Evaluation Gas Chromatography Generations Geometry Grant Health Heart Isomerism Laboratory Research Learning Libraries Liquid substance Mass Fragmentography Mass Spectrum Analysis Measurement Measures Methods Molecular Molecular Structure Molecular Weight Natural Product Drug Natural Products Paper Peer Review Performance Pharmaceutical Preparations Pharmacologic Substance Phase Protocols documentation Reference Standards Research Research Personnel Risk Rotation Sampling Series Shapes Small Business Innovation Research Grant Source Specificity Speed Structure System Techniques Technology Testing Tissues Toxin United States National Institutes of Health Water Work base biological systems commercialization design detector drug discovery enantiomer improved innovation insight instrument mass spectrometer metabolomics prototype screening small molecule

项目摘要

Gas Chromatography-Molecular Rotational Resonance Spectrometer NIH SBIR Direct to Phase II BrightSpec, Inc. Project Summary This project proposes the development of the first commercial gas chromatography-molecular rotational resonance (GC-MRR) spectrometer for analytical characterization of complex mixtures such as biological fluids and tissues, natural products, or environmental water sources. MRR is a spectroscopic technique for unambiguous structure determination and quantitation of small molecules, which has been used for decades in academic research laboratories but only recently commercialized by BrightSpec, driven by recent groundbreaking innovations. The key advantages of MRR motivating its incorporation into a gas chromatography hyphenated system are its selectivity to isomeric compounds, absolute quantitation capabilities, and identification of unknowns without reference standards. GC-MRR not only complements existing techniques (such as mass spectrometry and NMR), but also adds new insights to enhance discovery in numerous fields of research. A proof-of-concept prototype GC-MRR instrument has been used to demonstrate its capabilities to resolve complex mixtures, including co-eluting isotopologues and isotopomers. This preliminary work has de-risked this proposed Phase II project, where this prototype will be refined to develop a product with the sensitivity, speed, and ease-of-use necessary to facilitate its commercial adoption. This includes both the instrument hardware to quickly and precisely measure MRR spectra of analytes as they elute from the chromatographic column, and the software interface to allow users to easily set up measurements, visualize data, and interpret the results. The product will then be tested thoroughly on relevant samples in the market areas where GC-MRR is expected to have the greatest impact. Finally, following this extensive evaluation, the product design will be refined for commercial launch at or before the end of the grant period. The completed GC-MRR product holds the promise to enable breakthrough research in a number of high-impact health-related fields, including metabolomics, natural products drug discovery, and monitoring of environmental pollutants, among others.

气相色谱-分子旋转共振光谱仪 NIH SBIR直接进入II期 Jersey，Inc. 项目摘要该项目提出了第一个商业气相色谱-分子的发展用于复杂混合物分析表征的旋转共振（GC-MRR）光谱仪例如生物流体和组织、天然产物或环境水源。MRR是一个用于小分子化合物明确结构测定和定量的光谱技术分子，它已经在学术研究实验室中使用了几十年，但直到最近才开始使用。在最近突破性创新的推动下，关键优势 MRR促使其并入气相色谱联用系统的原因是其对异构体化合物的选择性、绝对定量能力和没有参考标准的未知数。GC-MRR不仅补充了现有技术（如质谱和核磁共振），而且还增加了新的见解，以提高发现在许多研究领域。一个概念验证原型GC-MRR仪器已被用来证明其能力解析复杂的混合物，包括共洗脱同位素体和同位素异构体。这初步工作已经降低了拟议的第二阶段项目的风险，在第二阶段，将对原型进行改进开发一种具有必要的灵敏度、速度和易用性的产品，商业收养。这包括快速精确测量的仪器硬件当分析物从色谱柱中排出时，分析物的MRR光谱，以及软件界面，使用户能够轻松地设置测量，可视化数据，并解释结果。的然后，将在GC-MRR适用的市场区域对相关样品进行彻底测试。预计将产生最大的影响。最后，经过广泛的评估，我们会在资助期结束时或之前改良设计，以便投放市场。完成的GC-MRR产品有望在许多领域实现突破性研究。高影响力的健康相关领域，包括代谢组学，天然产物药物发现，监测环境污染物等。