Developing a predictive understanding of harmful cyanbacteria growth, toxins production and comparative toxicity across environmentally important gradients of n:p and salinity

对环境重要的 n:p 和盐度梯度中有害蓝藻的生长、毒素产生和相对毒性进行预测性了解

• 批准号: 10218175
• 负责人: BRYAN WILLIAM BROOKS
• 金额: $ 17.31万
• 依托单位: UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-30 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10218175
• 关键词: Affect; Algae; Anabaena flos-aquae; Anatoxins; Antibiotic Resistance; Attention; Bacteria; Bacterial Infections; Chemicals; Code; Cyanobacterium; Cyanotoxin; Cylindrospermopsis; Development; Diagnosis; Disease; Disinfection; Droughts; Ecosystem; Environment and Public Health; Enzyme-Linked Immunosorbent Assay; Event; Exposure to; Floods; Frequencies; Fresh Water; Goals; Growth; Health; Human; Incidence; Inflammation; Knowledge; Lead; Linear Models; Liquid substance; Methods; Microcystis aeruginosa; Microscopic; Modeling; Monitor; Nitrogen; Non-linear Models; Nutrient; Oceans; Phosphorus; Play; Population; Precipitation; Prevention strategy; Production; Public Health; Public Health Schools; Recreation; Resources; Risk; Role; Safety; Saline; Saxitoxin; Seafood; South Carolina; Surface; Techniques; Temperature; Toxic effect; Toxin; Trace metal; United States Public Health Service; Universities; Vibrio; Vibrio Infections; Virulence; Water; climate change; comparative; cyanobacterial toxin; cyanoginosin LR; cylindrospermopsin; drinking water; experimental study; harmful algal blooms; hazard; health assessment; microcystin; non-alcoholic fatty liver disease; prevent; response; sea level rise; stoichiometry; tool; trait; water quality; water quality model

PROJECT SUMMARY This project will specifically support achieving the overarching goal of the University of South Carolina Center (USC Center) and its overall Specific Aim, which includes assessing the effects of climate change (through alterations in temperature, salinity, pH and biogeochemical cycling of trace metals and microplastics) on the antibiotic resistance and/virulence of Vibrio bacteria and the growth and toxins production by cyanobacteria that adversely affect drinking water, contact recreation and seafood safety exposure to humans, which may lead to increases in Vibrio infections, increased inflammation and disease (e.g., Non Alcoholic Liver Disease) in humans. Though we have known for decades that nutrient enrichment of surface waters can lead to excessive algal growth, including the development of harmful algal blooms (HABs), the causes and consequences of toxins produced by these blooms has recently received heightened attention from environmental public health practitioners. Nutrient enrichment, primarily from phosphorus (P) and nitrogen (N), increases the frequency and magnitude of blooms along the freshwater to marine continuum. However, less is known about how the stoichiometric interactions between N and P across environmentally relevant gradients, particularly in combination with salinity, may influence the growth, toxins production and comparative toxicity of cyanobacteria HABs. Climate change can affect incidents of HABs and salinity, which can be altered by both changes in precipitation (droughts or floods) and sea level rise. Whereas ecological studies and monitoring activities have previously examined “toxicity,” these efforts are routinely limited by absence of robust analytical quantitation of diverse toxins produced by specific HAB species and comparative toxicity exerted through multiple mechanisms of action including major alterations in water quality conditions resulting in differential risks to human health and ecosystems. This represents a critical consideration for management of water resources and protection of human health because algae growth does not necessarily predict toxins production, yet routine monitoring and surveillance activities, an essential environmental public health service, when these efforts do exist, use microscopic methods for cyanobacteria and thus do not quantify the presence of toxins. If toxins analysis occurs, it most commonly uses ELISA techniques to check for presence of microcystins. Further, commonly used water quality models lack inputs for toxins production, which inherently limits predictive capacity of HAB events. Some species of cyanobacteria have evolved unique adaptations to promote their growth under N-deficient conditions, but it remains unknown whether or not these traits actively exist simultaneously with toxins production. Developing predictive growth, toxins production and comparative toxicity models, proposed through the Specific Aims of this project, for cyanobacteria that commonly dominate toxic HAB events across relevant environmental gradients is thus imperative for forecasting, diagnosing and preventing human health risks presented by algal toxins, which appear to represent a transformative threat to water resources assessment and management.

项目摘要 该项目将特别支持实现南卡罗来纳大学中心的总体目标 （USC中心）及其总体特定目标，其中包括评估气候变化的影响（通过 痕量金属和微塑料的温度，盐度，pH和生物地球化学循环的改变） 纤维细菌的抗生素耐药性和/病毒以及蓝细菌的生长和毒素产生 这会对饮用水产生不利影响，接触娱乐和海鲜安全暴露于人类，这可能 导致弧菌感染增加，感染和疾病的增加（例如，非酒精性肝病） 在人类中。尽管我们已经知道几十年来，地表水的营养丰富会导致 过度藻类生长，包括有害藻类血（HAB）的发展，原因和原因 这些血液产生的毒素的后果最近受到了人们的关注。 环境公共卫生从业人员。营养富集，主要来自磷（P）和氮（N）， 增加了沿着淡水到海洋连续体的血液的频率和大小。但是，少是 知道如何在环境相关梯度之间N和P之间的化学计量相互作用， 特别是与盐度结合，可能会影响生长，毒素的产生和比较毒性 蓝细菌哈布斯。气候变化会影响HAB和盐度的事件，这两者都可以改变 降水（干旱或地板）和海平面上升的变化。而生态研究和监测 活动以前已经检查了“毒性”，这些努力通常会因缺乏强大的分析而受到限制 定量特定HAB物种产生的潜水毒素和通过比较毒性通过 多种作用机制，包括水质条件的重大变化，导致差异 人类健康和生态系统的风险。这代表了管理水管理的关键考虑因素 资源和对人类健康的保护，因为藻类的生长不一定预测毒素 生产，但常规监测和监视活动，这是一项重要的环境公共卫生服务， 当这些努力确实存在时，请使用微观方法进行蓝细菌，因此不量化存在 毒素。如果发生毒素分析，则最常见的是使用ELISA技术检查是否存在 微囊蛋白。此外，常用的水质模型缺乏毒素生产的投入 限制HAB事件的预测能力。某些种类的蓝细菌已经发展为 在N缺陷条件下促进其增长，但尚不清楚这些特征是否积极 与毒素产生相当简单。发展预测性生长，毒素产生和比较 通过该项目的特定目的提出的毒性模型，对于通常主导的蓝细菌 因此，相关环境梯度之间的有毒HAB事件对于预测，诊断和 防止藻类毒素带来的人类健康风险，这似乎代表了对 水资源评估和管理。