Changes in Oral and Gut Microbiota and Incidence and Severity of Patient-Reported Symptoms in Pre- and Post-Kidney Transplant Patients

肾移植前后患者口腔和肠道微生物群的变化以及患者报告症状的发生率和严重程度

• 批准号: 10219362
• 负责人: Mark Lockwood
• 金额: $ 16.19万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-25 至 2022-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10219362
• 关键词: 16S ribosomal RNA sequencing; Address; Affect; Age; Antibiotics; Antiviral Agents; Anxiety; Awareness; Bacteria; Birth; Body mass index; Cesarean section; Cessation of life; Chemicals; Chronic Kidney Failure; Communication; Communities; Complete Blood Count; Confounding Factors (Epidemiology); Creatinine; Data; Development; Dietary History; Dietary Intervention; Disease; Education; Ethnic Origin; Etiology; Exposure to; Family; Fatigue; Feces; Gastrointestinal tract structure; Gender; Gene Components; Genes; Glomerular Filtration Rate; Goals; Graft Rejection; Health; Human; Immunologics; Immunosuppression; Immunosuppressive Agents; Incidence; Income; Individual; Infection; Inflammatory; Intervention; Kidney Diseases; Kidney Transplantation; Life Style Modification; Link; Living Donors; Longitudinal Studies; Measurement; Measures; Mental Depression; Metabolic; Methods; Mission; Monitor; National Institute of Nursing Research; Nature; Neurotransmitters; Nurses; Nutritional status; Operative Surgical Procedures; Oral; Oral cavity; Organism; Outcome; Outcome Measure; Participant; Patient Outcomes Assessments; Patient-Focused Outcomes; Patients; Pharmaceutical Preparations; Phenotype; Process; Production; Prospective cohort study; Proteobacteria; Quality of life; Questionnaires; Race; Regimen; Renal function; Reporting; Research; Resolution; Sampling; Sampling Studies; Schedule; Severities; Shotguns; Sleep; Sleep Disorders; Sleep disturbances; Sputum; Structure; Symptoms; System; Tacrolimus; Taxonomy; Time; Transplant Recipients; Transplantation; Transplantation Surgery; Vagina; anxiety symptoms; beta diversity; cytokine; delayed graft function; design; diet and exercise; disabling symptom; experience; fecal microbiota; gut microbiome; gut microbiota; gut-brain axis; health related quality of life; improved; innovation; medication compliance; medication nonadherence; metagenomic sequencing; microbial community; microbiome; microbiome alteration; microbiome sequencing; microbiota; multidisciplinary; novel; novel strategies; oral microbial community; oral microbiome; personalized medicine; post-transplant; probiotic supplementation; prospective; reduce symptoms; restoration; symptom cluster; symptom science

Abstract This novel prospective cohort study will explore the relationship between kidney transplantation (antibiotics, antivirals, and immunosuppressants), changes in the composition and functional gene content of the microbiome, and the incidence and severity of patient reported symptoms before and after patients receive a kidney transplant from a live donor. We have selected living donor recipients to reduce the chance of missing samples, as these are scheduled surgeries. Thirty-nine subjects will provide stool and sputum samples for shot gun metagenomic sequencing of the microbiome before transplant, during the first week after transplant prior to discharge, and at 3 months after the transplant. Demographic (age, gender, race/ethnicity, income, education) and disease specific data (HT/WT/BMI, creatinine, estimated glomerular filtration rate, basic metabolic panel, complete blood count), and medication adherence data (electronic medication adherence monitoring and tacrolimus trough levels) will be collected. Potential confounding variables will include: historical use of antibiotics, concomitant medications, mode of delivery (vaginal vs. caesarian birth), and nutritional status (Diet History Questionnaire III). Incidence and severity of symptoms of fatigue, sleep dysfunction, and anxiety/depression–like symptoms will be measured using the Patient Reported Outcome Measurement Systems (PROMIS) 57 v2.1. Health related quality of life will be assessed using the Kidney Disease Quality of Life Short Form (KDQOL-SF v1.3). This research will support the mission of the National Institute of Nursing Research (NINR) to promote and improve the health and quality of life of individuals, families, and communities. The proposed study will contribute to the NINR mission by: 1) Exploring the mechanisms underlying incidence and severity of symptoms after kidney transplantation with the goal of developing personalized treatments, including pre-probiotics supplementation, lifestyle modification, and dietary interventions that address these mechanisms through symptom science research; 2) Helping individuals with chronic kidney disease/kidney transplant recipients better understand and manage these conditions by engaging individuals as active participants in managing their own health through awareness of modifiable factors that alter the microbiome; and 3) Promoting the development of innovative, multidisciplinary, nurse-led teams to advance research on microbiome and health after kidney transplantation.

摘要

项目成果

Clinical care is evolving: The microbiome for advanced practice nurses.

临床护理正在不断发展：高级执业护士的微生物组。

• DOI: 10.1097/jxx.0000000000000379
• 发表时间: 2020
• 期刊: Journal of the American Association of Nurse Practitioners
• 影响因子: 1.2
• 作者: Lockwood,MarkB;Green,StefanJ
• 通讯作者: Green,StefanJ

Metallochaperones Are Needed for Mycobacterium tuberculosis and Escherichia coli Nicotinamidase-Pyrazinamidase Activity.

结核分枝杆菌和大肠杆菌烟酰胺酶-吡嗪酰胺酶活性需要金属伴侣。

• DOI: 10.1128/jb.00331-19
• 发表时间: 2020
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Sheen,Patricia;Monsalve,Anuntxi;Campos,Jhanina;Huerta,Rodolfo;Antiparra,Ricardo;Arteaga,Héctor;Duran,Patricia;Bueno,Carlos;Kirwan,DanielaE;Gilman,RobertH;Zimic,Mirko
• 通讯作者: Zimic,Mirko