A novel in vivo functional imaging platform to study the re-animation of light responses in a blind eye

一种新颖的体内功能成像平台，用于研究盲眼中光反应的重新激活

• 批准号: 10219264
• 负责人: Kevin Cao
• 金额: $ 7.26万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-07 至 2022-08-06
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10219264
• 关键词: Action Potentials; Address; Affinity; Ammonium; Biological Assay; Bypass; Cells; Clinic; Communication; Complex; Cyclodextrins; Development; Disease; Electrophysiology (science); Evaluation; Eye; Fellowship; Fluorescence Microscopy; Functional Imaging; Goals; Image; Injections; Ion Channel; Lasers; Light; Mass Spectrum Analysis; Measures; Mediating; Methods; Microscope; Microscopy; Morphology; Mouse Strains; Mus; NMR Spectroscopy; Neurons; Ophthalmoscopes; Ophthalmoscopy; Optics; Photophobia; Photoreceptors; Photosensitization; Preparation; Reporting; Research; Rest; Retina; Retinal Cone; Retinal Ganglion Cells; Retinitis Pigmentosa; Scanning; Stimulus; Synapses; System; Techniques; Time; Tissues; Translations; Work; absorption; animation; azobenzene; base; behavioral study; blind; design; experimental study; imaging platform; improved; in vivo; in vivo imaging; insight; irradiation; multi-electrode arrays; novel; professor; relating to nervous system; response; restoration; retinal neuron; retinal rods; sight restoration; sugar; time use; two-photon; ultraviolet; voltage

Abstract Synthetic azobenzene photoswitches can bypass the degenerated rod and cone photoreceptors of a blind retina and directly photosensitize retinal ganglion cells (RGCs) to restore light-response. This proposal outlines a novel in vivo imaging platform that utilizes 2-photon tracking scanning laser ophthalmoscopy (2P-tSLO) and GCaMP6 Ca2+ imaging to visualize RGCs of blind rd1 mice that have reanimated light sensitivity. The research planned in this fellowship entails 1) the development of an optimized photoswitch delivery system using Ca2+ imaging and multielectrode array electrophysiology and 2) the evaluation of photoswitch light-restoration using in vivo Ca2+ imaging.

摘要