A novel in vivo functional imaging platform to study the re-animation of light responses in a blind eye

摘要 人工合成的偶氮苯光开关可以绕过盲人视网膜退化的视杆细胞和视锥细胞 并直接光敏视网膜神经节细胞(RGC)以恢复光反应。这份提案概述了一部小说 利用双光子跟踪扫描激光眼底镜(2P-tSLO)和GCaMP6的活体成像平台 钙离子成像用于显示具有复活光敏感度的失明Rd1小鼠的视网膜神经节细胞。这项研究计划在 这项研究包括：1)开发一种优化的光开关传输系统，使用钙离子成像和 多电极阵列电生理学及2)体内钙离子对光开关光恢复的评价 成像。

Abstract Synthetic azobenzene photoswitches can bypass the degenerated rod and cone photoreceptors of a blind retina and directly photosensitize retinal ganglion cells (RGCs) to restore light-response. This proposal outlines a novel in vivo imaging platform that utilizes 2-photon tracking scanning laser ophthalmoscopy (2P-tSLO) and GCaMP6 Ca2+ imaging to visualize RGCs of blind rd1 mice that have reanimated light sensitivity. The research planned in this fellowship entails 1) the development of an optimized photoswitch delivery system using Ca2+ imaging and multielectrode array electrophysiology and 2) the evaluation of photoswitch light-restoration using in vivo Ca2+ imaging.