Antibody Core

抗体核心

• 批准号: 10220154
• 负责人: MOSES VICTOR CHAO
• 金额: $ 32.92万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-15 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10220154
• 关键词: Antibodies; Antibody Formation; Area; BRAIN initiative; Behavior; Behavioral; Biochemistry; Brain; Communities; Core Facility; Data; Electron Microscopy; Ensure; Extramural Activities; Fc Receptor; Generations; Goals; Hippocampus (Brain); Hypothalamic structure; Individual; International; Methods; Molecular; Monoclonal Antibodies; Mus; Neuraxis; Oxytocin; Oxytocin Receptor; Peptide Signal Sequences; Physiological; Production; Quality Control; Rattus; Reagent; Receptor Signaling; Research Personnel; Research Support; Resource Sharing; Resources; Scientist; Signal Transduction; Social Behavior; Songbirds; Testing; Time; Tissues; Training; Validation; Work; antibody test; cell type; improved; medical schools; monoclonal antibody production; neural circuit; neuroregulation; novel; polyclonal antibody; receptor expression; scale up; success; web site

Project Summary: Antibody Core One of the major features of this BRAIN Initative proposal on “Oxytocin Modulation of Neural Circuit Function and Behavior” is this Oxytocin Receptor Antibody Production Research Support Core. Each of the Project teams is examining oxytocin release and the action of oxytocin receptor signaling. Oxytocin is one of the best- understood modulators at the physiological and behavioral levels, having been studied in various forms for over a century. However, little is known about the cellular and network effects of oxytocin signaling, in part due to previous lack of specific antibodies for determining which brain areas and cell types express oxytocin receptors. Our labs have generated, validated, and are distributing the first specific antibodies to mouse oxytocin receptors, and given the successful use and enthusiasm by the scientific community, we feel obliged to scale-up production of these reagents and improve their functionality. Aim 1 of the Antibody Core is to continue production of oxytocin receptor antibodies, Aim 2 is to distribute these antibodies broadly, and Aim 3 is to generate monoclonal antibodies to facilitate their broader use by Project labs and other investigators both in the US and internationally. There is clearly urgent need for these resources, which are best produced, tested, and distributed by a Core facility rather than by individual labs.

项目概要：抗体核心 BRAIN 倡议提案的主要特点之一是“神经回路功能的催产素调节” 和行为”是这个催产素受体抗体生产研究支持核心。各项目团队 正在检查催产素的释放和催产素受体信号传导的作用。催产素是最好的之一 了解生理和行为层面的调节剂，已经以各种形式进行了超过 一个世纪。然而，人们对催产素信号传导的细胞和网络效应知之甚少，部分原因是 以前缺乏特异性抗体来确定哪些大脑区域和细胞类型表达催产素受体。 我们的实验室已经生成、验证并正在分发第一个针对小鼠催产素受体的特异性抗体， 鉴于科学界的成功使用和热情，我们感到有必要扩大生产规模 这些试剂并改进其功能。抗体核心的目标 1 是继续产生催产素 受体抗体，目标 2 是广泛分布这些抗体，目标 3 是产生单克隆抗体 抗体，以促进美国和国际上的项目实验室和其他研究人员更广泛地使用它们。 显然迫切需要这些资源，这些资源最好由核心制作、测试和分发 设施而不是单个实验室。