Antibody Core

抗体核心

• 批准号: 10220154
• 负责人: MOSES VICTOR CHAO
• 金额: $ 32.92万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-15 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10220154
• 关键词: Antibodies; Antibody Formation; Area; BRAIN initiative; Behavior; Behavioral; Biochemistry; Brain; Communities; Core Facility; Data; Electron Microscopy; Ensure; Extramural Activities; Fc Receptor; Generations; Goals; Hippocampus (Brain); Hypothalamic structure; Individual; International; Methods; Molecular; Monoclonal Antibodies; Mus; Neuraxis; Oxytocin; Oxytocin Receptor; Peptide Signal Sequences; Physiological; Production; Quality Control; Rattus; Reagent; Receptor Signaling; Research Personnel; Research Support; Resource Sharing; Resources; Scientist; Signal Transduction; Social Behavior; Songbirds; Testing; Time; Tissues; Training; Validation; Work; antibody test; cell type; improved; medical schools; monoclonal antibody production; neural circuit; neuroregulation; novel; polyclonal antibody; receptor expression; scale up; success; web site

Project Summary: Antibody Core One of the major features of this BRAIN Initative proposal on “Oxytocin Modulation of Neural Circuit Function and Behavior” is this Oxytocin Receptor Antibody Production Research Support Core. Each of the Project teams is examining oxytocin release and the action of oxytocin receptor signaling. Oxytocin is one of the best- understood modulators at the physiological and behavioral levels, having been studied in various forms for over a century. However, little is known about the cellular and network effects of oxytocin signaling, in part due to previous lack of specific antibodies for determining which brain areas and cell types express oxytocin receptors. Our labs have generated, validated, and are distributing the first specific antibodies to mouse oxytocin receptors, and given the successful use and enthusiasm by the scientific community, we feel obliged to scale-up production of these reagents and improve their functionality. Aim 1 of the Antibody Core is to continue production of oxytocin receptor antibodies, Aim 2 is to distribute these antibodies broadly, and Aim 3 is to generate monoclonal antibodies to facilitate their broader use by Project labs and other investigators both in the US and internationally. There is clearly urgent need for these resources, which are best produced, tested, and distributed by a Core facility rather than by individual labs.

项目概要：抗体核心 这项关于“催产素调节神经回路功能"的BRAIN倡议提案的主要特点之一 和行为”是这个催产素受体抗体生产研究支持核心。每个项目小组 正在研究催产素的释放和催产素受体信号的作用。催产素是最好的- 在生理和行为水平上理解调节剂，已经以各种形式研究了超过 一个世纪然而，人们对催产素信号的细胞和网络效应知之甚少，部分原因是 以前缺乏特异性抗体来确定哪些大脑区域和细胞类型表达催产素受体。 我们的实验室已经产生、验证并分发了第一批针对小鼠催产素受体的特异性抗体， 鉴于科学界的成功使用和热情，我们觉得有必要扩大生产规模 并改善它们的功能。抗体核心的目标1是继续生产催产素 目的2是广泛分布这些抗体，目的3是产生单克隆抗体， 抗体，以促进项目实验室和其他研究人员在美国和国际上更广泛地使用它们。 显然，对这些资源的需求非常迫切，这些资源最好由一个核心来生产、测试和分发。 实验室，而不是个人实验室。