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Molecular Tools Core

分子工具核心

基本信息

批准号：
10705993
负责人：
MOSES VICTOR CHAO
金额：
$ 68.5万
依托单位：
NEW YORK UNIVERSITY SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-15 至 2028-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10705993
关键词：
Agonist Antibodies Area BRAIN initiative Behavior Behavioral Biochemistry Brain Central Nervous System Chemistry Communication Communities Core Facility Data Development Dissection Electron Microscopy Elements Ensure Epitopes Extramural Activities Fc Receptor Gene Expression Generations Genetic Genetic Models Goals Hippocampus Hypothalamic structure In Vitro Individual Infusion procedures International Intranasal Administration Letters Ligands Methods Microtus Molecular Monitor Monoclonal Antibodies Mouse Cell Line Mus Neuromodulator Neurons Optics Organism Oxytocin Oxytocin Receptor Peptide Signal Sequences Peptides Peripheral Physiological Population Postdoctoral Fellow Production Rattus Reagent Receptor Signaling Research Support Resources Rodent Role Signal Transduction Social Behavior Songbirds Spatial Behavior Specificity Study models System Testing Tissues Training Validation Venus Viral Visualization Work antagonist blood-brain barrier crossing cell type genetic approach improved in vivo innovation loss of function manufacturing scale-up medical schools member mouse model neural circuit neuroregulation novel peptide hormone receptor response sensor spatiotemporal tissue fixing tool transcriptome

项目摘要

Project Summary: Molecular Tools Core One of the major features of this BRAIN Initiative proposal on “Oxytocin Modulation of Neural Circuit Function and Behavior” is this Molecular Tools Research Support Core. Each of the Project teams is examining oxytocin release and the action of oxytocin receptor signaling. Oxytocin is exemplary among peptide hormones and neuromodulators, has been studied in various forms for over a century, and has clear physiological action, behavioral relevance, and biomedical importance. However, little is known about the cellular and network effects of oxytocin signaling. This is in part due to lack of specific antibodies for determining which brain areas and cell types express oxytocin receptors, as well as other molecular tools for specifically manipulating and monitoring oxytocin signaling with high spatiotemporal precision. Our labs have generated, validated, and are distributing the first specific antibodies to mouse oxytocin receptors, and have also developed the first successful versions of caged oxytocin compounds. Given the successful use and enthusiasm by the scientific community together with the need for continued validation and optimization, we feel obliged to scale-up production of these reagents and improve their functionality. There is clearly urgent and widespread need for these resources, which are best produced, tested, and distributed by a Core facility rather than by individual labs. Aim 1 of the Molecular Tools Core is to continue production of oxytocin receptor antibodies, distribute these antibodies broadly, and optimize their utility and specificity including generation of monoclonal antibodies. Aim 2 is to generate and optimize caged and photo-switchable forms of oxytocin receptor agonists and antagonists, useful for delineating specifically when and where oxytocin receptor signaling acts to generate physiological responses in the central nervous system and in peripheral tissues. Aim 3 generates reagents for click chemistry involving tagged oxytocin, to visualize oxytocin within fixed and live tissue, to help determine if and where exogenous oxytocin delivery acts within an organism. Aim 4 is to generate useful and when needed develop new mouse lines for cell-type specific dissection of oxytocinergic signaling, and to leverage transcriptome data and other gene expression resources to facilitate studies on the roles of molecularly defined Oxt+ and Oxtr+ populations in socio-spatial behavior.

项目概述：Molecular Tools Core