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Understanding signal processing in retinal bipolar cell pathways

了解视网膜双极细胞通路中的信号处理

基本信息

批准号：
10222697
负责人：
Bart Gerard Borghuis
金额：
$ 36.54万
依托单位：
UNIVERSITY OF LOUISVILLE
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-09-01 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10222697
关键词：
Address Aging Amacrine Cells Area Axon Biosensor Brain Calcium Cell physiology Cells Electrophysiology (science)Equilibrium Feedback Glutamate Receptor Glutamates Glycine Goals Image Individual Kainic Acid Receptors Kinetics Knowledge Light Light Adaptations Lighting Maps Measures Methods Molecular Natural regeneration Neurons Optic Nerve Output Pathway interactions Photic Stimulation Photoreceptors Preparation Process Property Retina Retinal Diseases Retinal Ganglion Cells Role Route Series Shapes Signal Pathway Signal Transduction Slice Stimulus Stratification Structure Synapses Testing Transgenic Mice Vision Visual Visual system structure Whole-Cell Recordings base cell type developmental disease experimental study extrastriate visual cortex fluorescence imaging gamma-Aminobutyric Acid ganglion cell information processing innovation live cell imaging loss of function mutation luminance mouse model nervous system disorder neural circuit neuromechanism parallel processing postsynaptic preservation prevent receptive field receptor response retinal rods signal processing spatiotemporal transmission process two-photon visual information visual processing voltage voltage gated channel

项目摘要

We have developed a powerful mouse model to study information processing in parallel retinal bipolar cell pathways. Bipolar cells are essential for transmitting the photoreceptor output to the ganglion cells, which signal visual information to a range of target areas in the brain. Bipolar cells divide into about a dozen types and constitute the first level of information processing in the visual system. While several aspects of bipolar cell function have been addressed in retinal slice preparations of a variety of species, information about three fundamental aspects of bipolar cell signaling is still lacking. First is a concise, quantitative description of the spatio-temporal receptive field for any genetically identified bipolar cell type. Second is a comprehensive account of the neural mechanisms that govern the input-output transfer function of each BC type, based on light-evoked responses with intact circuitry (whole-mount retina preparation). Third is the impact of light-adaptive mechanisms on bipolar cell visual function. We will leverage our recently developed live-cell imaging and whole-cell recording methods to address these issues. Specifically, we will make targeted electrophysiological recordings in the whole-mount retina of transgenic mice with genetically identified bipolar cells; we will use two-photon fluorescence imaging with genetically encoded fluorescent biosensors for calcium and glutamate in transgenic mice with loss-of function mutations in the OFF bipolar pathways; and we will use advanced visual stimulation methods to elucidate mechanisms of light-adaptation at the bipolar cell level. These studies will provide valuable information about how bipolar cells encode visual information, and how visual sensitivity is maintained under varying stimulus conditions. Understanding information processing in identified retinal signaling pathways is critical for the study and potential treatment of developmental and neurological disorders, and retinal disease.

我们建立了一个强大的小鼠模型来研究平行视网膜双极细胞的信息处理