Monoclonal antibody isolation and characterization

单克隆抗体分离和表征

• 批准号: 10275374
• 负责人: Rosemarie Mason
• 金额: $ 83.47万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10275374
• 关键词: Antibodies; Antibody Therapy; Autologous; CD8-Positive T-Lymphocytes; Cellular immunotherapy; Characteristics; Data; Dependovirus; Exhibits; Fc Receptor; Gene Transfer; Immunotherapy; In Vitro; Individual; Intervention; Methods; Modeling; Monoclonal Antibodies; Mus; Passive Immunization; Prevention; Preventive; Reagent; Techniques; Testing; Therapeutic; Virus; Virus Diseases; base; chimeric antibody; clinical development; clinically relevant; efficacy testing; experimental study; in vivo evaluation; nonhuman primate; pathogen; pathogenic virus; prevent; single cell technology; therapeutic evaluation; tool

TTo date, we have isolated nearly 200 monoclonal antibodies and characterized their functionality in vitro. Approximately half a dozen of these have been made into chimeric antibody receptors and tested in vitro. A few of those were down selected for in vivo testing, which is ongoing. In addition, we have made two distinct adeno associated viruses expressing 2 monoclonal antibodies. we have shown that these express antibodies to high levels in mice, and in pilot experiments in nonhuman primates. Challenge experiments in this latter model are underway. Finally, we have initiated experiments to test the therapeutic as well as preventive potency of these antibodies delivered by passive immunization.

到目前为止，我们已经分离了近200种单克隆抗体，并在体外表征了它们的功能。其中大约有六种已被制成嵌合抗体受体并进行了体外测试。其中一些被选中进行体内测试，目前正在进行中。此外，我们还制备了两种不同的腺相关病毒，表达2种单克隆抗体。我们已经在小鼠和非人类灵长类动物的初步实验中表明，这些细胞表达高水平的抗体。后一种模式的挑战性实验正在进行中。最后，我们已经启动了实验来测试通过被动免疫递送的这些抗体的治疗以及预防效力。