Monoclonal antibody isolation and characterization for COVID-19 Effort

COVID-19 的单克隆抗体分离和表征

• 批准号: 10930481
• 负责人: Rosemarie Mason
• 金额: $ 13.15万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10930481
• 关键词: 2019-nCoV; Antibody Therapy; Antigens; COVID-19; Cell Separation; Complex; Coronavirus; Development; Dissociation; Epitopes; In Vitro; Monoclonal Antibodies; Prevention; Publications; Resolution; SARS-CoV-2 antibody; SARS-CoV-2 infection; Severe Acute Respiratory Syndrome; Structure; Virus Diseases; neutralizing monoclonal antibodies; vaccine development; variants of concern

To date, we have isolated more than 100 SARS-CoV-2-specific monoclonal antibodies (mAbs) from SARS-CoV-2 convalescent donors and characterized their functionality in vitro. More than 25 of the mAbs isolated and characterized were neutralizing against SARS-CoV-2 with several showing pan-coronavirus neutralization (i.e. the ability to neutralize SARS-CoV-2, multiple Variants of Concern (VOC) as well as SARS1 and other SARS1- and SARS2-related coronaviruses). Structures of 5 pan-coronavirus neutralizing mAbs were solved, revealing that 2 of the mAbs disrupt/dissociate the spike trimer and target a relatively conserved epitope. Results describing the isolation, characterization, and high resolution structures of our pan-coronavirus neutralizing mAbs in complex with SARS-CoV-2 spike, are being prepared for publication.

迄今为止，我们已经从SARS-CoV-2恢复期供体中分离出100多种SARS-CoV-2特异性单克隆抗体（mab），并在体外鉴定了它们的功能。超过25个分离和鉴定的单克隆抗体对SARS-CoV-2具有中和作用，其中一些单克隆抗体表现出泛冠状病毒中和作用（即能够中和SARS-CoV-2、多种关注变体（VOC）以及SARS1和其他SARS1和sars2相关的冠状病毒）。

项目成果

OMIP 074: Phenotypic analysis of IgG and IgA subclasses on human B cells.

• DOI: 10.1002/cyto.a.24341
• 发表时间: 2021-09
• 期刊: Cytometry. Part A : the journal of the International Society for Analytical Cytology
• 作者: Nettey L;Ballard R;Liechti T;Mason RD
• 通讯作者: Mason RD