Monoclonal antibody isolation and characterization

迄今为止，我们已经分离出200多种单克隆抗体，并在体外鉴定了它们的功能。其中大约有六种已被制成嵌合抗体受体，并在体外进行了测试。其中一些被选中进行正在进行的体内试验。此外，我们还制备了两种不同的表达两种单克隆抗体的腺相关病毒。我们已经在小鼠和非人灵长类动物的初步实验中证明了这些抗体的高水平表达。后一种模式的挑战实验正在进行中。最后，我们已经启动了实验来测试这些抗体通过被动免疫传递的治疗和预防效力。

To date, we have isolated more than 200 monoclonal antibodies and characterized their functionality in vitro. Approximately half a dozen of these have been made into chimeric antibody receptors and tested in vitro. A few of those were down selected for in vivo testing, which is ongoing. In addition, we have made two distinct adeno associated viruses expressing 2 monoclonal antibodies. we have shown that these express antibodies to high levels in mice, and in pilot experiments in nonhuman primates. Challenge experiments in this latter model are underway. Finally, we have initiated experiments to test the therapeutic as well as preventive potency of these antibodies delivered by passive immunization.