Monoclonal antibody isolation and characterization

单克隆抗体分离和表征

• 批准号: 10497746
• 负责人: Rosemarie Mason
• 金额: $ 59.03万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10497746
• 关键词: Antibodies; Antibody Therapy; Autologous; CD8-Positive T-Lymphocytes; Cellular immunotherapy; Characteristics; Data; Dependovirus; Exhibits; Fc Receptor; Gene Transfer; Immunotherapy; In Vitro; Individual; Intervention; Methods; Modeling; Monoclonal Antibodies; Mus; Passive Immunization; Prevention; Preventive; Reagent; Techniques; Testing; Therapeutic; Virus; Virus Diseases; antibody test; base; chimeric antibody; clinical development; clinically relevant; efficacy testing; experimental study; in vitro testing; in vivo evaluation; nonhuman primate; pathogen; pathogenic virus; prevent; single cell technology; therapeutic evaluation; tool

To date, we have isolated more than 200 monoclonal antibodies and characterized their functionality in vitro. Approximately half a dozen of these have been made into chimeric antibody receptors and tested in vitro. A few of those were down selected for in vivo testing, which is ongoing. In addition, we have made two distinct adeno associated viruses expressing 2 monoclonal antibodies. we have shown that these express antibodies to high levels in mice, and in pilot experiments in nonhuman primates. Challenge experiments in this latter model are underway. Finally, we have initiated experiments to test the therapeutic as well as preventive potency of these antibodies delivered by passive immunization.

到目前为止，我们已经分离了200多个单抗，并在体外鉴定了它们的功能。其中大约六个已经制成嵌合抗体受体，并在体外进行测试。其中几个被选中进行体内测试，这一测试正在进行中。此外，我们还制备了两种不同的表达两种单抗的腺相关病毒。我们已经证明，这些细胞在小鼠身上表达高水平的抗体，在非人类灵长类动物的初步实验中也是如此。后一种模式的挑战实验正在进行中。最后，我们已经开始了实验，以测试被动免疫所提供的这些抗体的治疗和预防效力。