Biophysical models and mechanisms for cellular adaptation to environmental stress

细胞适应环境压力的生物物理模型和机制

基本信息

  • 批准号:
    10276814
  • 负责人:
  • 金额:
    $ 31.22万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-08-01 至 2026-05-31
  • 项目状态:
    未结题

项目摘要

Project Summary/Abstract Living cells possess the remarkable ability to adapt to changes in their environmental conditions. Adaptation involves changes in cellular properties in response to external cues in order to regulate vital physiological functions and processes. While much progress has been made in identifying the molecular components and biochemical pathways underlying cellular stress response, the role of cellular physical properties in adaptive stress response is mostly unknown. Our recent studies provide evidence that changes in cell shape and cellular physical properties promotes adaptive benefits in certain stressful conditions via mechanochemical feedback processes. The goal of the proposed research is to develop quantitative theory and data-driven computational models to uncover the biophysical feedback mechanisms underlying cellular adaptive response to environmental stresses. We will utilize an interdisciplinary approach that integrates tools from statistical physics, systems biology, and experimental data analysis to construct predictive models for cell behavior. We will specifically investigate adaptive response in two different biological systems: 1) adaptation to nutrient shifts and antibiotic stresses in proliferating bacterial cells, and 2) adaptation to energy deprivation and cell state transitions in nematode worm embryos. In each of these systems we will develop quantitative cell-level models based on known molecular circuits, intracellular biophysical interactions and dynamics observed in experimental data. The models will be calibrated and tested against quantitative single-cell data obtained from our experimental collaborators. The resultant models will help test different experimental hypotheses, isolate and test the relative roles of biochemical and physical pathways in cellular adaptive response, and pinpoint the main driving forces behind complex adaptive phenomena. In addition to developing quantitative models for cellular biophysical behaviors, our study will generate a variety of computational tools that will enable efficient whole-cell simulations of bacterial growth, morphogenesis, intracellular organization and single-cell development.
项目总结/摘要 活细胞具有适应环境变化的非凡能力。适应 涉及细胞特性的变化,以响应外部线索,以调节重要的生理 功能和过程。虽然在鉴定分子组分方面取得了很大进展, 细胞应激反应的生化途径,细胞物理特性在适应性 压力反应大多未知。我们最近的研究提供了证据表明,细胞形状和细胞形态的变化, 物理性质通过机械化学反馈促进某些应激条件下的适应性益处 流程.该研究的目标是发展定量理论和数据驱动的计算 模型,以揭示细胞对环境适应性反应的生物物理反馈机制。 压力我们将利用跨学科的方法,整合统计物理学,系统 生物学和实验数据分析,以构建细胞行为的预测模型。我们将特别 研究两种不同生物系统适应性反应:1)对营养变化和抗生素的适应 在增殖的细菌细胞中的压力,和2)适应能量剥夺和细胞状态转换, 线虫胚胎在这些系统中,我们将开发基于以下内容的定量细胞水平模型: 已知的分子电路,细胞内生物物理相互作用和实验数据中观察到的动力学。的 模型将被校准,并根据从我们的实验中获得的定量单细胞数据进行测试。 合作者由此产生的模型将有助于测试不同的实验假设,隔离和测试相对的 生物化学和物理途径在细胞适应性反应中的作用,并确定主要驱动力 复杂的适应现象背后。除了开发细胞生物物理学的定量模型外, 行为,我们的研究将产生各种计算工具,使高效的全细胞模拟 细菌生长、形态发生、细胞内组织和单细胞发育。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Shiladitya Banerjee其他文献

Shiladitya Banerjee的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Shiladitya Banerjee', 18)}}的其他基金

Biophysical models and mechanisms for cellular adaptation to environmental stress
细胞适应环境压力的生物物理模型和机制
  • 批准号:
    10458693
  • 财政年份:
    2021
  • 资助金额:
    $ 31.22万
  • 项目类别:
Biophysical models and mechanisms for cellular adaptation to environmental stress
细胞适应环境压力的生物物理模型和机制
  • 批准号:
    10622566
  • 财政年份:
    2021
  • 资助金额:
    $ 31.22万
  • 项目类别:

相似海外基金

New technologies for targeted delivery of anti-bacterial agents
抗菌药物靶向递送新技术
  • 批准号:
    1654774
  • 财政年份:
    2015
  • 资助金额:
    $ 31.22万
  • 项目类别:
    Studentship
Targeting bacterial phosphatases for novel anti-bacterial agents.
针对细菌磷酸酶的新型抗菌剂。
  • 批准号:
    8416313
  • 财政年份:
    2012
  • 资助金额:
    $ 31.22万
  • 项目类别:
Targeting bacterial phosphatases for novel anti-bacterial agents.
针对细菌磷酸酶的新型抗菌剂。
  • 批准号:
    8298885
  • 财政年份:
    2012
  • 资助金额:
    $ 31.22万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了