OPTIONAL QUANTITIES FOR CCG AND OCCPR PROGRAM OFFICES FOR ADDITIONAL SEQUENCING; TO PERIOD OF PERFORMANCE: 08/31/2019 - 08/30/2021O

北卡罗来纳大学教堂山分校（UNC）高通量测序设施（HTSF）提议成为基因组表征中心（GCC），从癌症相关样本中生产RNA测序数据（RNAseq）。癌症是一种异质性的疾病集合，具有各种各样的结果、自然历史和对治疗的反应。最近的数据表明，基于核酸的生物标志物是了解、预防、诊断和治疗癌症的宝贵工具。肿瘤全基因组基因表达谱是一种极其重要的数据类型，可以识别不同的疾病亚型，并为识别癌症的遗传驱动因素提供额外的支持。

The University of North Carolina at Chapel Hill (UNC) High-throughput Sequencing Facility (HTSF) proposes to become a Genome Characterization Center (GCC) for production of RNA sequencing data (RNAseq) from cancer related samples. Cancer is a heterogeneous collection of diseases with a wide variety of outcomes, natural histories and responses to therapy. Recent data show that nucleic acid-based biomarkers are invaluable tools for understanding, preventing, diagnosing, and treating cancer. Genome-wide gene expression profiling of tumors is an extremely important data type that can identify distinct subtypes of disease and provide additional support for the identification of the genetic drivers of cancers. The UNC HTSF has provided high quality, comprehensive RNAseq data from tumor and related samples for more than a decade. HTSF was the primary RNAseq GCC for The Cancer Genome Atlas (TCGA) project, which comprehensively cataloged the genomic and (epi)genetic alterations associated with 28 human cancers. Since the end of TCGA, UNC has continued to serve as the primary sub-contractor for RNAseq for Leidos Biomedical Research (LBR). In this role, it has sequenced RNA from thousands of RNA samples in support of several NCI projects. From this past work, HTSF has developed robust and reproducible protocols for miRNA, mRNA and Total RNA sequencing from fresh frozen and FFPE RNA, sophisticated sample tracking, and innovative analysis algorithm role to the samples described in this RFP.