Multiple Sessions of Regenerative Electrical Stimulation to Accelerate Sensory Neuron Regeneration

项目摘要 周围神经损伤（PNI）影响男性和女性在他们的生命的黄金时期，与损失 运动和感觉功能以及神经性疼痛。再生电刺激已被证明 加速PNI后的功能恢复临床前研究表明，多个一小时 再生促进电刺激的疗程可以进一步加速运动功能恢复， 但对感觉再生有不利影响。一项调查，以确定一组参数 这将有利于运动和感觉是必要的，所以多个再生刺激会话， 成为一种可能的治疗方法。为了评估不同长度的刺激会话，一些再生 将评估相关基因：甘丙肽（GAL），LIF和GAP 43，但以前的研究没有 评价电刺激治疗后的GAL或LIF。我假设， 刺激将允许增加刺激会话的数量 增强LIF和GAL表达。为了评估这一点，我将确定如果LIF和GAL下降- 在单侧坐骨神经后进行三小时的再生促进电刺激后调节 损伤;如果是，LIF和GAL与GAP 43将用于确定一组多个参数 刺激期促进感觉神经再生。表达式将通过 LIF、GAL和GAP 43的RNAscope，这是我将从Zigmond实验室学习的技术。的 然后将使用所找到的参数来确定它们是否加速再生。再生将得以 通过冯·弗雷细丝进行评估，这是我将从齐格蒙德实验室学到的另一种技术。

Project Summary Peripheral nerve injuries (PNIs) affect men and women in the prime of their lives, with the loss of motor and sensory function and neuropathic pain. Regenerative electrical stimulation has been shown to accelerate functional recovery after a PNI. Preclinical studies have shown that multiple one-hour sessions of regenerative promoting electrical stimulation can further accelerate motor function recovery, but have a detrimental effect on sensory regeneration. An investigation to determine a set of parameters that would benefit both motor and sensory is needed so multiple regenerative stimulation sessions can become a possible treatment. To evaluate different lengths of stimulation sessions, a few regenerative associated genes will be evaluated: galanin (GAL), LIF, and GAP 43, but previous studies have not evaluated GAL or LIF after electrical stimulation treatment. I hypothesize that decreasing the length of the stimulation will allow for increasing the number of stimulation sessions while still allowing enhanced LIF and GAL expression. To assess this, I will determine if LIF and GAL are down- regulated after three hours of regenerative promoting electrical stimulation after a unilateral sciatic nerve injury; if so, LIF and GAL with GAP 43 will be used to determine a set of parameters for multiple stimulation session that promote sensory nerve regeneration. The expression will be determined via the RNAscope of LIF, GAL, and GAP 43, a technique I will learn from the Zigmond laboratory. The parameters found will then be used to determine if they accelerate regeneration. Regeneration will be assessed via von Fray filaments, another technique I will learn from the Zigmond lab.