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Generation of a Light Inducible Cre Transgenic Animal for KidneyResearch

用于肾脏研究的光诱导 Cre 转基因动物的产生

基本信息

批准号：
10286292
负责人：
John M Parant
金额：
$ 22.28万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-07-15 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10286292
关键词：
Acute Address Alleles Animal Disease Models Animal Model Area Biochemical Biological Markers Biological Models Biosensor Cartoons Cell Culture Techniques Cells Complementary DNA Cyst Defect Development Disease Disease Progression Drosophila genus Environment Epithelial Freedom Future Generations Genetic Genetic Recombination Genome Goals Hematopoietic Hormones In Situ Individual Kidney Knock-out Label Light Loss of Heterozygosity Methods Modeling Monitor Mus Mutation Optics Organism Partner in relationship Pathogenicity Phase Phenotype Polycystic Kidney Diseases Pre-Clinical Model Proteins Reporter Research Research Personnel Research Project Grants Signal Pathway System Tamoxifen Temperature Testing Tissues Transgenes Transgenic Animals Transgenic Organisms Transplantation Whole Organism Zebrafish base cDNA Expression cell type conditional knockout data access disorder prevention genetic manipulation in vitro testing interest knockout animal loss of function migration optogenetics phase 2 testing promoter protein expression recombinase-mediated cassette exchange single cell analysis tissue injury tool urologic

项目摘要

ABSTRACT Animal modeling of disease states and bioreporters for in situ analysis of cellular dynamics are an essential aspect of kidney, urological and hematopoietic research; however, most studies focus on genetic manipulation within the whole organism or kidney specific cell types, which does not accurately reflect disease states that are often associated with defects in individual cells or groups of cells. This discrepancy is largely due to the technical chal- lenges of being able to unrestrictedly induce Cre recombination in an optically selected group of cells within an intact organism. Therefore, we believe that a system for controlled genetic manipulation (resulting in loss of function alleles or expression of exogenous cDNAs/bioreporters) within a group of cells of a viable organism is required. This system should not alter or perturb the surrounding environment and should allow for potential systems based analysis and biomarker incorporation. Therefore, we hypothesize that transgenic lines ubiqui- tously expressing a light inducible Cre would allow for such unseen optical spatial and temporal control within a living organism. This Light Inducible Recombination (LIR) systems will be established within both the mouse and the zebrafish model organisms. Upon completion of this proposal we will have established versatile and efficient tools for selective genetic alterations that will facilitate regional and potentially single cell analysis in a multitude of research fields. We anticipate that this research will have a broad positive impact on a number areas of kidney, urological and hematopoietic research.

摘要