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Schlemm’s canal targeted-Tie2 knockdown as a mouse model of adult-onset ocular hypertension and glaucoma

施累姆氏管靶向 Tie2 敲除作为成人发病高眼压症和青光眼的小鼠模型

基本信息

批准号：
10289450
负责人：
ALEJANDRA BOSCO
金额：
$ 22.88万
依托单位：
UNIVERSITY OF UTAH
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-01 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10289450
关键词：
Address Adult Alleles Amacrine Cells Angiopoietins Animal Model Apoptosis Autopsy Axon Blindness CRISPR/Cas technology Cell Density Cell physiology Chronic Defect Disease Down-Regulation Drops Elderly Endothelial Cells Enterobacteria phage P1 Cre recombinase Event Eye Functional disorder Genes Genetic Glaucoma Human Hypertension In Vitro Individual Injections Interruption Investigation Knock-out Link LoxP-flanked allele Lymphatic Measures Mediating Methods Modeling Molecular Mus Mutation Nerve Nerve Degeneration Neurodegenerative Disorders Ocular Hypertension Optic Nerve Optics Pathogenesis Pathogenicity Pathway interactions Penetrance Physiologic Intraocular Pressure Preclinical Testing Primary Open Angle Glaucoma Promoter Regions Reproducibility Retina Retinal Ganglion Cells Risk Rodent Rodent Model Signal Transduction Single Nucleotide Polymorphism Staphylococcus aureus Structure Structure of sinus venosus of sclera TIE-2 Receptor Testing Time Validation Vision Visual Visual Acuity adeno-associated viral vector anterior chamber axonal degeneration base cell injury design functional decline genetic approach high intraocular pressure human disease innovation knock-down mouse model neuronal cell body nonhuman primate pre-clinical prevent primary congenital glaucoma receptor expression retinal damage retinal ganglion cell degeneration tool

项目摘要

Glaucoma causes irreversible vision loss and blindness, but we still lack understanding of the multipart mechanisms that cause and drive glaucoma progression. Animal models enable investigation of cellular and molecular players throughout disease, and are essential for testing and optimizing new treatments. There is a need for animal models that selectively model pathogenic events of human glaucoma to trigger neurodegeneration with a predictable time of onset and progression. We have developed a mouse model that selectively disrupts Schlemm’s canal (SC) expression of Tie2, which is associated with ocular hypertension and glaucoma in humans. The studies proposed here will define the timecourse, sequence and variability of functional and structural optic nerve and RGC neurodegeneration in this model. We will also develop a CRISPR/Cas9 approach to disrupt Tie2 expression in SC, which could be applied independent of genetic background. This project is expected to provide an inducible glaucoma model relevant to study the pathogenesis of ocular hypertension and neurodegeneration, and a preclinical tool to evaluate new treatments for human glaucoma.

青光眼导致不可逆转的视力丧失和失明，但我们仍然缺乏对其各个部分的了解导致和推动青光眼进展的机制。动物模型能够研究细胞和分子在疾病中的作用，对于测试和优化新的治疗方法是必不可少的。有一个需要选择性模拟人类青光眼致病事件的动物模型来触发神经退行性变具有可预测的发病和进展时间。我们已经开发出一种老鼠模型，选择性干扰Schlemm管(SC)Tie2的表达，与高眼压有关以及人类的青光眼。这里提出的研究将定义时间进程、顺序和变异性功能和结构视神经及RGC神经退行性变。我们还将开发一种 CRISPR/Cas9方法阻断Tie2在SC中的表达，该方法可以独立于基因应用背景资料。该项目有望提供一种诱导性青光眼模型，用于研究高眼压和神经退行性变的发病机制和临床前评估新疗法的工具治疗人类青光眼。