Developing an ultrafast fluorescence lifetime imaging ophthalmoscopy system for retinal imaging

开发用于视网膜成像的超快荧光寿命成像检眼镜系统

• 批准号: 10288094
• 负责人: Hsin-Chih Yeh
• 金额: $ 22.22万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10288094
• 关键词: Acute; Adopted; Affect; Age related macular degeneration; Algorithms; American; Animal Model; Biochemistry; Centers for Disease Control and Prevention (U.S.); Clinic; Clinical; Computer software; Consumption; Data; Development; Diagnosis; Engineering; Eye; Eye diseases; Fluorescence; Frequencies; Functional Imaging; Fundus; Ganglion Cell Layer; Generations; Glaucoma; Goals; Gold; Hour; Hypoxia; Image; Impairment; Joints; Lead; Lighting; Measures; Metabolic; Methods; Microscopy; Modeling; Motivation; Nerve Degeneration; Ophthalmoscopy; Optical Coherence Tomography; Optics; Oryctolagus cuniculus; Photons; Photoreceptors; Physiologic pulse; Process; Publications; Reporting; Research; Retina; Retinal Diseases; Retinal Ganglion Cells; Role; Scanning; Scheme; Structure; System; Testing; Texas; Time; Universities; Vision; austin; base; biomarker identification; curve fitting; deep learning; deep learning algorithm; design; digital; experience; experimental study; fluorescence lifetime imaging; fluorophore; functional loss; in vivo; instrumentation; interest; microscopic imaging; next generation; reconstruction; retinal imaging; technology development; tool

PROJECT SUMMARY Age-related macular degeneration (AMD) is an eye disease that affects the central part of the retina and leads to impairments in central vision and sharp vision. According to the report from Centers for Disease Control and Prevention, AMD affects ~0.8% of people in US between 50 and 60, 1.5% between 60 and 70, ~4.8% between 70 and 80, and nearly 12% over 80 years old. Fluorescence lifetime imaging ophthalmoscopy (FLIO) has become an important tool in retinal diagnosis as it provides critical information about retina that the traditional fundus autofluorescence microscopy cannot provide. Whereas more demonstrations of FLIO in retina diagnosis at different stages of AMD have been shown in recent years, FLIO has not reached widespread use in clinics due to a number of fundamental limitations. Here we are developing an ultrafast fluorescence lifetime imaging ophthalmoscopy (uFLIO) system for rapid acquisition of retinal fundus autofluorescence and reconstruction of fluorescence lifetime images around the posterior pole of retina. The acquired autofluorescence lifetime information will help us understand the states of the endogenous fluorophores within the retina, and their roles and changes during the development of retinal diseases.

项目摘要 视网膜相关性黄斑变性（AMD）是一种影响视网膜中央部分的眼病， 中央视觉和敏锐视觉的损伤。根据疾病控制中心的报告， 预防，AMD影响美国约0.8%的50 - 60岁人群，1.5%的60 - 70岁人群，约4.8%的 70岁和80岁，80岁以上的占近12%。荧光寿命成像检眼镜（FLIO）已成为 它是视网膜诊断中的重要工具，因为它提供了传统眼底检查 自体荧光显微镜无法提供。而FLIO在视网膜诊断中的更多演示， 尽管近年来已经显示出AMD的不同阶段，但FLIO尚未在临床上广泛使用， 一些基本的限制。在这里，我们正在开发一个超快荧光寿命成像 用于快速采集视网膜眼底自发荧光和重建视网膜的眼底镜检查（uFLIO）系统 视网膜后极周围的荧光寿命图像。获得的自发荧光寿命 这些信息将帮助我们了解视网膜内内源性荧光团的状态及其作用 以及视网膜疾病发展过程中的变化。