Demonstrating the mechanism of Nairovirus translation strategy

展示内罗病毒翻译策略的机制

基本信息

  • 批准号:
    10291623
  • 负责人:
  • 金额:
    $ 42.3万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-08-01 至 2024-07-31
  • 项目状态:
    已结题

项目摘要

Abstract: Crimean Congo hemorrhagic fever is a tick born, highly contagious, viral illness with high mortality rates in humans. There is no treatment for this viral disease at present. We recently reported that Crimean Congo hemorrhagic fever virus nucleocapsid protein (CCHFV N protein) has two distinct RNA binding sites in the stalk and head domains. The RNA binding site located in the head domain non-specifically binds to the single strand RNA of viral or nonviral origin. However, the RNA binding site located in the stalk domain specifically binds to the double strand panhandle structure formed by the base pairing of highly conserved and complementary nucleotides at the 5’ and 3’ termini of the viral genome. Interestingly the viral mRNA 5’ UTR also folds into a panhandle-like secondary structure, which is also specifically recognized by the stalk domain of N protein. The interaction between N protein and viral mRNA 5’ UTR facilitates the translation of downstream open reading frame (ORF). The majority of eukaryotic mRNA translation is m7G cap dependent and is initiated by the assembly of eIF4F cap binding complex, composed of three initiation factors eIF4E, eIF4A and eIF4G. Our preliminary data shows that N protein mediated translation strategy does not require the assembly of eIF4F complex but the structural integrity of individual components of this complex is required for this viral translation mechanism. This published data suggests that CHFV N protein highly likely lures the host translation apparatus for the preferential translation of viral mRNA during the course of infection, to boost the translation of viral mRNA in the infected cell. We will use multifaceted experimental avenues to test the hypothesis that CCHFV N protein interacts with the components of eIF4F complex to selectively engage the 40S ribosomal subunits on the viral mRNA 5’ UTR. Since ribosome loading on mRNA is a critical rate limiting step in eukaryotic translation, CCHFV N protein likely helps the viral transcripts at this critical step by selectively engaging the host cell ribosomes on viral mRNA 5’ UTR. This selective ribosome loading likely helps viral transcripts by avoiding the competition from host cell transcripts for the same host translation machinery. We will determine whether CCHFV N protein mediated translation strategy selectively facilitates the translation of viral mRNA during the course of infection.
摘要:克里米亚刚果出血热是一种蜱媒传播的高传染性病毒性疾病,死亡率高

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Modulations in the host cell proteome by the hantavirus nucleocapsid protein.
  • DOI:
    10.1371/journal.ppat.1011925
  • 发表时间:
    2024-01
  • 期刊:
  • 影响因子:
    6.7
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Mohammad A Mir其他文献

Infant Sleep Positioning and SIDS: A Hospital Based Interventional Study
婴儿睡眠姿势与婴儿猝死综合征:一项基于医院的干预性研究
  • DOI:
    10.1203/00006450-199904020-00636
  • 发表时间:
    1999-04-01
  • 期刊:
  • 影响因子:
    3.100
  • 作者:
    Bharath Srivatsa;Alvin N Eden;Mohammad A Mir
  • 通讯作者:
    Mohammad A Mir

Mohammad A Mir的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Mohammad A Mir', 18)}}的其他基金

Preferential translation of host cell factors by hantavirus nucleocapsid protein
汉坦病毒核衣壳蛋白优先翻译宿主细胞因子
  • 批准号:
    9292026
  • 财政年份:
    2017
  • 资助金额:
    $ 42.3万
  • 项目类别:
Characterization of hantavirus N protein-mediated translation mechanism
汉坦病毒N蛋白介导的翻译机制的表征
  • 批准号:
    8218779
  • 财政年份:
    2012
  • 资助金额:
    $ 42.3万
  • 项目类别:
Characterization of hantavirus N protein-mediated translation mechanism
汉坦病毒N蛋白介导的翻译机制的表征
  • 批准号:
    8424961
  • 财政年份:
    2012
  • 资助金额:
    $ 42.3万
  • 项目类别:
Identification and characterization of inhibitors for hantavirus replication
汉坦病毒复制抑制剂的鉴定和表征
  • 批准号:
    8309019
  • 财政年份:
    2011
  • 资助金额:
    $ 42.3万
  • 项目类别:
Identification and characterization of inhibitors for hantavirus replication
汉坦病毒复制抑制剂的鉴定和表征
  • 批准号:
    8508844
  • 财政年份:
    2011
  • 资助金额:
    $ 42.3万
  • 项目类别:
Identification and characterization of inhibitors for hantavirus replication
汉坦病毒复制抑制剂的鉴定和表征
  • 批准号:
    8150134
  • 财政年份:
    2011
  • 资助金额:
    $ 42.3万
  • 项目类别:
MECHANICS OF HANTAVIRAL NUCLEOCAPSID PROTEIN MEDIATED TRANSLATION INITIATION OF
汉病毒核衣壳蛋白介导的翻译起始机制
  • 批准号:
    8168405
  • 财政年份:
    2010
  • 资助金额:
    $ 42.3万
  • 项目类别:
Role of Cellular P-bodies and Hantavirus Nucleocapsid Protein in Viral mRNA "cap
细胞P体和汉坦病毒核衣壳蛋白在病毒mRNA“帽”中的作用
  • 批准号:
    7701441
  • 财政年份:
    2009
  • 资助金额:
    $ 42.3万
  • 项目类别:
Role of Cellular P-bodies and Hantavirus Nucleocapsid Protein in Viral mRNA "cap
细胞P体和汉坦病毒核衣壳蛋白在病毒mRNA“帽”中的作用
  • 批准号:
    7897814
  • 财政年份:
    2009
  • 资助金额:
    $ 42.3万
  • 项目类别:

相似海外基金

Impact of alternative polyadenylation of 3'-untranslated regions in the PI3K/AKT cascade on microRNA
PI3K/AKT 级联中 3-非翻译区的替代多聚腺苷酸化对 microRNA 的影响
  • 批准号:
    573541-2022
  • 财政年份:
    2022
  • 资助金额:
    $ 42.3万
  • 项目类别:
    University Undergraduate Student Research Awards
How do untranslated regions of cannabinoid receptor type 1 mRNA determine receptor subcellular localisation and function?
1 型大麻素受体 mRNA 的非翻译区如何决定受体亚细胞定位和功能?
  • 批准号:
    2744317
  • 财政年份:
    2022
  • 资助金额:
    $ 42.3万
  • 项目类别:
    Studentship
MICA:Synthetic untranslated regions for direct delivery of therapeutic mRNAs
MICA:用于直接递送治疗性 mRNA 的合成非翻译区
  • 批准号:
    MR/V010948/1
  • 财政年份:
    2021
  • 资助金额:
    $ 42.3万
  • 项目类别:
    Research Grant
Translational Control by 5'-untranslated regions
5-非翻译区域的翻译控制
  • 批准号:
    10019570
  • 财政年份:
    2019
  • 资助金额:
    $ 42.3万
  • 项目类别:
Translational Control by 5'-untranslated regions
5-非翻译区域的翻译控制
  • 批准号:
    10223370
  • 财政年份:
    2019
  • 资助金额:
    $ 42.3万
  • 项目类别:
Translational Control by 5'-untranslated regions
5-非翻译区域的翻译控制
  • 批准号:
    10455108
  • 财政年份:
    2019
  • 资助金额:
    $ 42.3万
  • 项目类别:
Synergistic microRNA-binding sites, and 3' untranslated regions: a dialogue of silence
协同的 microRNA 结合位点和 3 非翻译区:沉默的对话
  • 批准号:
    255762
  • 财政年份:
    2012
  • 资助金额:
    $ 42.3万
  • 项目类别:
    Operating Grants
Analysis of long untranslated regions in Nipah virus genome
尼帕病毒基因组长非翻译区分析
  • 批准号:
    20790351
  • 财政年份:
    2008
  • 资助金额:
    $ 42.3万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Search for mRNA elements involved in the compatibility between 5' untranslated regions and coding regions in chloroplast translation
寻找参与叶绿体翻译中 5 非翻译区和编码区之间兼容性的 mRNA 元件
  • 批准号:
    19370021
  • 财政年份:
    2007
  • 资助金额:
    $ 42.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Post-transcriptional Regulation of PPAR-g Expression by 5'-Untranslated Regions
5-非翻译区对 PPAR-g 表达的转录后调控
  • 批准号:
    7131841
  • 财政年份:
    2006
  • 资助金额:
    $ 42.3万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了