Transcriptional, functional, and circuit profiling at single cell resolution of neuronal ensembles engaged by heroin relapse
海洛因复吸所涉及的神经元群的单细胞分辨率转录、功能和回路分析
基本信息
- 批准号:10292403
- 负责人:
- 金额:$ 56.99万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-07-01 至 2026-03-31
- 项目状态:未结题
- 来源:
- 关键词:AddressAffectAnatomyAnimal BehaviorAnimal ModelAtlasesBehaviorBioinformaticsBiological AssayBrainBrain regionCalciumCellsChromatinCommunitiesCuesDataData CollectionData SetDevelopmentDorsalDrug ExposureDrug ModelingsDrug usageExposure toFluorescent in Situ HybridizationFutureGene ExpressionGene Expression ProfileGene Expression ProfilingGenesGenetic TranscriptionGoldHeroinHeroin DependenceHumanImageIndividualIndividual DifferencesInfusion proceduresKnowledgeLeadLightMeasurementMedialMicroscopyModelingMolecularNatureNervous system structureNeuronsNeurosciencesNucleus AccumbensOpiate AddictionOpioidPatternPharmaceutical PreparationsPopulationProcessPublishingRNARattusRelapseResearchResolutionResourcesSamplingSelf AdministrationSeriesSignal TransductionSliceSocietiesStandardizationSystemThalamic structureTissue-Specific Gene ExpressionTissuesaddictionbasebehavioral phenotypingbrain cellbrain tissuecell typecohortdrug developmentdrug seeking behaviorin vivoin vivo calcium imagingmolecular markerneural circuitnovelopioid userelating to nervous systemresponsesingle cell sequencingsingle-cell RNA sequencingtherapeutic candidatetherapeutic target
项目摘要
Abstract:
Opiate addiction extorts a tremendous toll on society, but a mechanistic understanding of how repeated exposure
to opioids such as heroin ultimately results in compulsive drug-taking and -seeking behavior in some individuals,
but not others, is still not known. A longstanding idea is that enduring changes in neural circuit function occur
because of drug-induced gene expression changes in certain brain cells. This facilitates subsequent drug-taking
and -seeking behaviors in vulnerable individuals. Unfortunately, identifying cell-type specific alterations following
drug use (typically performed in established animal models of addiction), is generally a slow and tedious process
as changes in gene expression following in vivo drug exposure are typically assayed in series, within
heterogeneous brain regions, in an a-priori hypothesis driven fashion (i.e. previous knowledge predicting a
specific gene may be involved). This dramatically limits the throughput of data collection and likely complicates
the subsequent interpretation as gene expression patterns data are typically captured from thousands to millions
of homogenized cells. Given that the nervous system is composed of highly heterogeneous tissue, re-assessing
cell type specific gene expression changes in an unbiased manner from 1000's of individual cells is desperately
needed. Here, we propose to combine our expertise in order to generate comprehensive datasets aimed at
understanding how single-cell gene expression, circuit connectivity, and neural activity patterns are impacted by
previous drug-taking behavior. These data will provide a much-needed cellular atlas and resource for the
addiction neuroscience community and will likely lead to the identification of many novel cell type, gene
expression changes, and ensembles that can be leveraged for future study.
文摘:
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Susan Marie Ferguson其他文献
Susan Marie Ferguson的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Susan Marie Ferguson', 18)}}的其他基金
Characterization of cortical neuronal subtypes in cocaine self-administration
可卡因自我给药皮质神经元亚型的特征
- 批准号:
10815221 - 财政年份:2023
- 资助金额:
$ 56.99万 - 项目类别:
Assessing the role of corticostriatal circuitry in polysubstance use of fentanyl and methamphetamine using rat self-administration models
使用大鼠自我给药模型评估皮质纹状体回路在芬太尼和甲基苯丙胺多物质使用中的作用
- 批准号:
10737092 - 财政年份:2023
- 资助金额:
$ 56.99万 - 项目类别:
Characterization of cortical neuronal subtypes in cocaine self-administration
可卡因自我给药皮质神经元亚型的特征
- 批准号:
10893672 - 财政年份:2023
- 资助金额:
$ 56.99万 - 项目类别:
University of Washington Significant Opportunities in Addiction Research (UW-SOAR) Neuroscience Doctoral Readiness Program
华盛顿大学成瘾研究的重大机会(UW-SOAR)神经科学博士准备计划
- 批准号:
10706601 - 财政年份:2022
- 资助金额:
$ 56.99万 - 项目类别:
University of Washington Significant Opportunities in Addiction Research (UW-SOAR) Neuroscience Doctoral Readiness Program
华盛顿大学成瘾研究的重大机会(UW-SOAR)神经科学博士准备计划
- 批准号:
10610060 - 财政年份:2022
- 资助金额:
$ 56.99万 - 项目类别:
Transcriptional, functional, and circuit profiling at single cell resolution of neuronal ensembles engaged by heroin relapse
海洛因复吸所涉及的神经元群的单细胞分辨率转录、功能和回路分析
- 批准号:
10596142 - 财政年份:2021
- 资助金额:
$ 56.99万 - 项目类别:
Transcriptional, functional, and circuit profiling at single cell resolution of neuronal ensembles engaged by heroin relapse
海洛因复吸所涉及的神经元群的单细胞分辨率转录、功能和回路分析
- 批准号:
10434119 - 财政年份:2021
- 资助金额:
$ 56.99万 - 项目类别:
Characterization of cortical neuronal subtypes in cocaine self-administration
可卡因自我给药皮质神经元亚型的特征
- 批准号:
10171832 - 财政年份:2019
- 资助金额:
$ 56.99万 - 项目类别:
Characterization of cortical neuronal subtypes in cocaine self-administration
可卡因自我给药皮质神经元亚型的特征
- 批准号:
10350049 - 财政年份:2019
- 资助金额:
$ 56.99万 - 项目类别:
Characterization of cortical neuronal subtypes in cocaine self-administration
可卡因自我给药皮质神经元亚型的特征
- 批准号:
10627077 - 财政年份:2019
- 资助金额:
$ 56.99万 - 项目类别:
相似海外基金
RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
- 批准号:
2327346 - 财政年份:2024
- 资助金额:
$ 56.99万 - 项目类别:
Standard Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
- 批准号:
2312555 - 财政年份:2024
- 资助金额:
$ 56.99万 - 项目类别:
Standard Grant
How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
- 批准号:
BB/Z514391/1 - 财政年份:2024
- 资助金额:
$ 56.99万 - 项目类别:
Training Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
- 批准号:
ES/Z502595/1 - 财政年份:2024
- 资助金额:
$ 56.99万 - 项目类别:
Fellowship
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
- 批准号:
ES/Z000149/1 - 财政年份:2024
- 资助金额:
$ 56.99万 - 项目类别:
Research Grant
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
- 批准号:
23K24936 - 财政年份:2024
- 资助金额:
$ 56.99万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
- 批准号:
2901648 - 财政年份:2024
- 资助金额:
$ 56.99万 - 项目类别:
Studentship
ERI: Developing a Trust-supporting Design Framework with Affect for Human-AI Collaboration
ERI:开发一个支持信任的设计框架,影响人类与人工智能的协作
- 批准号:
2301846 - 财政年份:2023
- 资助金额:
$ 56.99万 - 项目类别:
Standard Grant
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
- 批准号:
488039 - 财政年份:2023
- 资助金额:
$ 56.99万 - 项目类别:
Operating Grants
How motor impairments due to neurodegenerative diseases affect masticatory movements
神经退行性疾病引起的运动障碍如何影响咀嚼运动
- 批准号:
23K16076 - 财政年份:2023
- 资助金额:
$ 56.99万 - 项目类别:
Grant-in-Aid for Early-Career Scientists