Cellular Components of Human Milk: An Examination of Their Role in Infant Health and Development and the Functional Impact of Hospital Storage Practices

母乳的细胞成分：检查其在婴儿健康和发育中的作用以及医院储存实践的功能影响

• 批准号: 10301555
• 负责人: Carrie-Ellen Briere
• 金额: $ 14.63万
• 依托单位: UNIVERSITY OF MASSACHUSETTS AMHERST
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-26 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10301555
• 关键词: Address; Admission activity; Affect; Animal Model; Area; Biological; Birth; Brain; Bronchopulmonary Dysplasia; Cell Death; Cell physiology; Cells; Characteristics; Clinical; Clinical Research; Development; Ensure; Epithelial Cell Proliferation; Face; Fostering; Freezing; Future; Gastrointestinal tract structure; Genes; Goals; Growth; Growth and Development function; Health; Heart; Hospitals; Human Milk; Immune response; Immunologics; Impairment; Infant; Infant Development; Infant Health; Ingestion; Intervention; Intestines; Knowledge; Laboratories; Lactation; Life; Ligands; Lung; Methods; Milk; Modeling; Mothers; Necrotizing Enterocolitis; Neonatal; Neonatal Intensive Care Units; Neurological outcome; Newborn Infant; Organ; Outcome; Physiological; Play; Population; Premature Birth; Premature Infant; Property; Refrigeration; Research; Research Project Grants; Research Proposals; Research Support; Research Technics; Research Training; Risk; Role; Science; Severities; Supporting Cell; Technical Expertise; Training; Translational Research; Travel; Work; biological systems; body system; career; cell type; cytokine; donor milk; feeding; improved; innovation; interest; intestinal epithelium; laboratory experience; late onset sepsis; mammary; meetings; microbial; milk expression; mouse model; neonatal care; organ growth; pasteurization; personalized medicine; pluripotency; premature; programs; protective effect; regenerative; stem; stem cells; stem-like cell; tissue culture

Project Summary/Abstract Preterm infants are at increased risk of health complications due to their under-developed organ systems at birth, and human milk receipt is recognized as an important intervention to promote infant health. Recent identification of cells in human milk which have similar characteristics to stem cells, as well as animal models which show integration of these cells into nursling's organs, indicate an important function of milk cells which is currently not understood. In the NICU, infants often receive human milk which has been refrigerated or frozen, and then warmed and/or thawed prior to feeding. The impact of these typical handling practices on the cellular components of milk that may provide an important mechanism of biologic protection in infant health has not been investigated. This work will determine how the real-life storage and handling of human milk impacts infant health and development, and is the first to evaluate protective mechanisms. There are currently two major knowledge gaps in the fields of human milk and lactation and neonatal care surrounding bioactive cellular components. The first is a lack of knowledge of how real-life storage and handling practices impact the protective ability of milk's cellular components for infants. Without this knowledge, infants who may benefit from milk with the most bioactivity (such as preterm infants) may not receive the properties of their mother's milk which provide important protection. The second major opportunity is the lack of knowledge on the mechanism(s) of protection in milk stem-like cells. Additionally, as research techniques advance our ability to investigate milk components, there is a great need to look at milk components in a biological systems perspective. The specific aims of the research project are to 1) examine how hospital storage practices (refrigeration and freezing) impact the protective mechanisms of human milk cells through the use of a tissue culture model of intestinal health, and 2) determine if milk cells are integrated as functioning cells specific to vital organs impacted by preterm birth (brain, heart, lungs, intestine) using a cross-foster mouse model. Upon completion of the specific aims of the K23 research strategy and training plans, the candidate will have advanced theoretical knowledge and technical skills to conduct human milk research with the ability to apply a biological systems approach to understand the complexity of the many components of milk which likely impact the function of each. The proposal research aims are supported by research training, didactic coursework, scientific meetings, and specific plans for dissemination and future growth. Ultimately, the short- and long-term goals of this research are to 1) improve infant health by optimizing the delivery of the most bioactive human milk components and 2) determine the mechanism(s) by which human milk cells protect infants and promotes growth and development.

项目概要/摘要 早产儿由于器官系统发育不全，出现健康并发症的风险增加 出生和接受母乳被认为是促进婴儿健康的重要干预措施。最近的 鉴定母乳中与干细胞具有相似特征的细胞以及动物模型 显示这些细胞整合到幼儿器官中，表明乳细胞的重要功能是 目前不明白。在新生儿重症监护病房（NICU）中，婴儿经常接受冷藏或冷冻的母乳， 然后在喂养前加热和/或解冻。这些典型处理实践对蜂窝的影响 可能为婴儿健康提供重要生物保护机制的牛奶成分尚未被证实 被调查。这项工作将确定现实生活中母乳的储存和处理如何影响婴儿 健康和发展，并且是第一个评估保护机制的人。目前主要有两个 母乳、哺乳和新生儿护理领域围绕生物活性细胞的知识差距 成分。首先是缺乏对现实生活中的存储和处理实践如何影响的了解。 牛奶细胞成分对婴儿的保护能力。如果没有这些知识，婴儿可能会受益 最具生物活性的乳汁（例如早产儿）可能无法获得母乳的特性 提供重要的保护。第二个重大机会是缺乏对相关知识的了解。 乳汁干细胞样细胞的保护机制。此外，随着研究技术的进步，我们的能力 研究牛奶成分，非常需要研究生物系统中的牛奶成分 看法。该研究项目的具体目标是 1) 研究医院的存储实践 （冷藏和冷冻）通过使用组织影响母乳细胞的保护机制 肠道健康的培养模型，2) 确定乳细胞是否整合为特定的功能细胞 使用交叉寄养小鼠模型研究受早产影响的重要器官（大脑、心脏、肺、肠）。之上 完成K23研究策略和培训计划的具体目标后，候选人将拥有 先进的理论知识和技术技能，能够进行母乳研究并具有应用能力 生物系统方法来了解牛奶中许多成分的复杂性，这些成分可能会影响 各自的功能。该提案的研究目标得到研究培训、教学课程、 科学会议以及传播和未来发展的具体计划。最终，短期和长期 这项研究的目标是 1) 通过优化最具生物活性的人类的分娩来改善婴儿健康 乳成分和 2) 确定母乳细胞保护婴儿和促进婴儿生长的机制 成长和发展。