Cellular Components of Human Milk: An Examination of Their Role in Infant Health and Development and the Functional Impact of Hospital Storage Practices

母乳的细胞成分：检查其在婴儿健康和发育中的作用以及医院储存实践的功能影响

• 批准号: 10456969
• 负责人: Carrie-Ellen Briere
• 金额: $ 14.62万
• 依托单位: UNIVERSITY OF MASSACHUSETTS AMHERST
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-26 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10456969
• 关键词: Address; Admission activity; Affect; Animal Model; Area; Biological; Birth; Brain; Bronchopulmonary Dysplasia; Cell Death; Cell physiology; Cells; Characteristics; Clinical; Clinical Research; Development; Ensure; Epithelial Cell Proliferation; Face; Fostering; Freezing; Future; Gastrointestinal tract structure; Genes; Goals; Growth; Growth and Development function; Health; Heart; Hospitals; Human Milk; Immune response; Immunologics; Impairment; Infant; Infant Development; Infant Health; Ingestion; Intervention; Intestines; Knowledge; Laboratories; Lactation; Life; Ligands; Lung; Methods; Milk; Modeling; Mothers; Necrotizing Enterocolitis; Neonatal; Neonatal Intensive Care Units; Neurological outcome; Newborn Infant; Organ; Outcome; Physiological; Play; Population; Premature Birth; Premature Infant; Property; Refrigeration; Research; Research Project Grants; Research Proposals; Research Support; Research Technics; Research Training; Risk; Role; Science; Severities; Supporting Cell; Technical Expertise; Training; Translational Research; Travel; Work; biological systems; body system; career; cell type; cytokine; donor milk; feeding; improved; innovation; interest; intestinal epithelium; laboratory experience; late onset sepsis; mammary; meetings; microbial; milk expression; mouse model; neonatal care; organ growth; pasteurization; personalized medicine; pluripotency; premature; programs; protective effect; regenerative; stem; stem cells; stem-like cell; tissue culture

Project Summary/Abstract Preterm infants are at increased risk of health complications due to their under-developed organ systems at birth, and human milk receipt is recognized as an important intervention to promote infant health. Recent identification of cells in human milk which have similar characteristics to stem cells, as well as animal models which show integration of these cells into nursling's organs, indicate an important function of milk cells which is currently not understood. In the NICU, infants often receive human milk which has been refrigerated or frozen, and then warmed and/or thawed prior to feeding. The impact of these typical handling practices on the cellular components of milk that may provide an important mechanism of biologic protection in infant health has not been investigated. This work will determine how the real-life storage and handling of human milk impacts infant health and development, and is the first to evaluate protective mechanisms. There are currently two major knowledge gaps in the fields of human milk and lactation and neonatal care surrounding bioactive cellular components. The first is a lack of knowledge of how real-life storage and handling practices impact the protective ability of milk's cellular components for infants. Without this knowledge, infants who may benefit from milk with the most bioactivity (such as preterm infants) may not receive the properties of their mother's milk which provide important protection. The second major opportunity is the lack of knowledge on the mechanism(s) of protection in milk stem-like cells. Additionally, as research techniques advance our ability to investigate milk components, there is a great need to look at milk components in a biological systems perspective. The specific aims of the research project are to 1) examine how hospital storage practices (refrigeration and freezing) impact the protective mechanisms of human milk cells through the use of a tissue culture model of intestinal health, and 2) determine if milk cells are integrated as functioning cells specific to vital organs impacted by preterm birth (brain, heart, lungs, intestine) using a cross-foster mouse model. Upon completion of the specific aims of the K23 research strategy and training plans, the candidate will have advanced theoretical knowledge and technical skills to conduct human milk research with the ability to apply a biological systems approach to understand the complexity of the many components of milk which likely impact the function of each. The proposal research aims are supported by research training, didactic coursework, scientific meetings, and specific plans for dissemination and future growth. Ultimately, the short- and long-term goals of this research are to 1) improve infant health by optimizing the delivery of the most bioactive human milk components and 2) determine the mechanism(s) by which human milk cells protect infants and promotes growth and development.

项目摘要/摘要 早产儿健康并发症的风险增加，因为他们的器官系统不发达 分娩和母乳接收被认为是促进婴儿健康的重要干预措施。近期 鉴定母乳中具有与干细胞相似特征的细胞以及动物模型 表明这些细胞整合到哺乳器官中，表明乳细胞的一项重要功能是 目前还不清楚。在NICU中，婴儿经常接受冷藏或冷冻的母乳， 然后在喂食之前加热和/或解冻。这些典型处理做法对蜂窝网络的影响 可能为婴儿健康提供生物保护的重要机制的牛奶成分尚未 被调查过了。这项工作将确定现实生活中母乳的储存和处理如何影响婴儿 健康和发展，并是第一个评估保护机制的机构。目前有两个主要的 在母乳和哺乳以及围绕生物活性细胞的新生儿护理领域的知识差距 组件。首先是缺乏对现实生活中的存储和搬运做法如何影响 牛奶中细胞成分对婴儿的保护作用。如果没有这方面的知识，可能受益于 生物活性最强的牛奶(如早产儿)可能得不到其母乳的特性 它们提供了重要的保护。第二个主要机会是缺乏关于 乳干细胞的保护机制(S)。此外，随着研究技术的进步，我们能够 研究牛奶的成分，很有必要研究牛奶的生物系统中的成分 透视。该研究项目的具体目的是：1)检查医院的存储做法 (冷藏和冷冻)通过使用组织影响人类乳细胞的保护机制 肠道健康的培养模型，以及2)确定牛奶细胞是否整合为特定于 采用交叉寄养小鼠模型，观察早产对重要器官(脑、心、肺、肠)的影响。vt.在.的基础上 完成K23研究策略和培训计划的具体目标后，应聘者将拥有 先进的理论知识和技术技能，能够进行母乳研究，并能应用 用生物系统的方法来了解牛奶中可能影响的许多成分的复杂性 每一项的功能。建议的研究目标由研究培训、教学课程、 科学会议，以及传播和未来增长的具体计划。归根结底，短期和长期 这项研究的目标是1)通过优化最活跃的人类的分娩来改善婴儿的健康 2)确定母乳细胞保护婴儿和促进婴儿生长的机制(S) 成长和发展。